Restriction map for
pF1KB4911
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# NO CUT ENZYMES ( 180 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaII AviII AvrII BalI
BamHI BanI BbeI BbrPI BcgI
BclI BcnI BfaI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaJI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BsmFI BsmI Bsp1407I BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssKI BssSI Bst1107I BstAPI BstBI
BstEII BstNI BstPI BstXI BstYI
BstZ17I Bsu36I Cac8I CelII Cfr10I
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRII EcoT14I EcoT22I FauI
FbaI FseI FspI HapII HincII
HindII HindIII HpaI HpaII KasI
KpnI KspI MaeI MaeII MaeIII
MamI MfeI MflI MluI MluNI
MroI MscI MslI MspI MunI
MvaI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NlaIV NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI ScrFI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI StyI SwaI
TfiI Tsp45I Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 453 CGCG CG^CG
AcyI 508 GACGCC G[GA]^CG[CT]C
AlwI 91 GATCC GGATC(4/5)
Asp700I 193 GAACGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 13 GAGCTC G[AT]GC[AT]^C
AvaI 31 CTCGGG C^[CT]CG[GA]G
BanII 13 GAGCTC G[GA]GC[CT]^C
BbvCI 6 GCTGAGG CCTCAGC(-5/-2)
BmyI 13 GAGCTC G[AGT]GC[ACT]^C
BsaHI 508 GACGCC G[GA]^CG[CT]C
BsaI 436 GAGACC GGTCTC(1/5)
BsiHKAI 13 GAGCTC G[AT]GC[AT]^C
BsoBI 31 CTCGGG C^[CT]CG[GA]G
Bsp1286I 13 GAGCTC G[AGT]GC[ACT]^C
BspDI 367 ATCGAT AT^CGAT
BspMI 515 ACCTGC ACCTGC(4/8)
BsrDI 342 CATTGC GCAATG(2/0)
BsrI 141 CCAGT ACTGG(1/-1)
BstF5I 500 CATCC GGATG(2/0)
BstUI 453 CGCG CG^CG
Cfr13I 319 GGCCC G^G[ACGT]CC
ClaI 367 ATCGAT AT^CGAT
EarI 268 GAAGAG CTCTTC(1/4)
EcoRI 494 GAATTC G^AATTC
EcoRV 256 GATATC GAT^ATC
FokI 500 CATCC GGATG(9/13)
HaeII 3 GGCGCT [GA]GCGC^[CT]
HaeIII 319 GGCC GG^CC
HgaI 508 GACGC GACGC(5/10)
Hin1I 508 GACGCC G[GA]^CG[CT]C
HinfI 412 GACTC G^A[ACGT]TC
HphI 297 GGTGA GGTGA(8/7)
Ksp632I 268 GAAGAG CTCTTC(1/4)
MseI 286 TTAA T^TAA
MspA1I 182 CAGCTG C[AC]G^C[GT]G
MvnI 453 CGCG CG^CG
PleI 412 GACTC GAGTC(4/5)
PstI 236 CTGCAG CTGCA^G
PvuII 182 CAGCTG CAG^CTG
SacI 13 GAGCTC GAGCT^C
Sau96I 319 GGCCC G^G[ACGT]CC
SfcI 236 CTGCAG C^T[GA][CT]AG
SspI 328 AATATT AAT^ATT
Tru9I 286 TTAA T^TAA
TspRI 504 CACTG CA[GC]TG[ACGT][ACGT]^
XmnI 193 GAACGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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AcsI 163 AAATTC [GA]^AATT[CT]
494 GAATTC
AfaI 144 GTAC GT^AC
219 GTAC
ApoI 163 AAATTC [GA]^AATT[CT]
494 GAATTC
BbsI 79 GAAGAC GAAGAC(2/6)
548 GAAGAC
BciVI 36 GGATAC GTATCC(6/5)
177 GGATAC
BpuAI 79 GAAGAC GAAGAC(2/6)
548 GAAGAC
BsmAI 271 GAGAC GTCTC(1/5)
436 GAGAC
CfoI 4 GCGC GCG^C
443 GCGC
DdeI 7 CTGAG C^T[ACGT]AG
207 CTTAG
DpnII 91 GATC ^GATC
361 GATC
HhaI 4 GCGC GCG^C
443 GCGC
HinP1I 4 GCGC G^CGC
443 GCGC
MboI 91 GATC ^GATC
361 GATC
MnlI 9 GAGG CCTC(7/6)
227 GAGG
NdeII 91 GATC ^GATC
361 GATC
RsaI 144 GTAC GT^AC
219 GTAC
Sau3AI 91 GATC ^GATC
361 GATC
SfaNI 394 GATGC GCATC(5/9)
118 GCATC
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# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
AciI AluI BbvI BsiYI BslI
Fnu4HI ItaI MboII MwoI NlaIII
TaqI TseI Tsp509I TthHB8I