Restriction map for
pF1KB4908
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AhdI Aor51HI AscI AseI AsnI
Asp718I AspEI AspI AviII BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BsaBI BsaI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KpnI KspI MaeIII MamI MfeI
MroI MunI NdeI NheI NotI
NruI NsiI NspV PacI PflFI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SmaI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tsp45I
Tth111I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AatII 517 GACGTC GACGT^C
AocI 540 CCTCAGG CC^T[ACGT]AGG
ApaI 331 GGGCCC GGGCC^C
AvrII 243 CCTAGG C^CTAGG
BalI 1751 TGGCCA TGG^CCA
BlnI 243 CCTAGG C^CTAGG
BsaAI 1789 TACGTG [CT]AC^GT[GA]
BsmI 838 GAATGC GAATGC(1/-1)
BssSI 1378 CTCGTG CACGAG(-5/-1)
BstXI 866 CCAACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 540 CCTCAGG CC^T[ACGT]AGG
EaeI 1751 TGGCCA [CT]^GGCC[GA]
EarI 553 CTCTTC CTCTTC(1/4)
Eco81I 540 CCTCAGG CC^T[ACGT]AGG
EcoRI 532 GAATTC G^AATTC
HincII 173 GTCAAC GT[CT]^[GA]AC
HindII 173 GTCAAC GT[CT]^[GA]AC
Ksp632I 553 CTCTTC CTCTTC(1/4)
MluNI 1751 TGGCCA TGG^CCA
MscI 1751 TGGCCA TGG^CCA
MslI 1564 CACACCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 707 GCCGGC GCC^GGC
NgoMIV 707 GCCGGC G^CCGGC
PaeR7I 76 CTCGAG C^TCGAG
PflMI 506 CCATGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SmlI 76 CTCGAG C^T[CT][GA]AG
Van91I 506 CCATGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1844 CCATCGACGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 76 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 532 GAATTC [GA]^AATT[CT]
1902 GAATTT
AflIII 567 ACGCGT A^C[GA][CT]GT
1367 ACGCGT
AgeI 1079 ACCGGT A^CCGGT
1567 ACCGGT
ApoI 532 GAATTC [GA]^AATT[CT]
1902 GAATTT
BamHI 48 GGATCC G^GATCC
1839 GGATCC
BbsI 27 GAAGAC GAAGAC(2/6)
98 GTCTTC
BfaI 178 CTAG C^TAG
244 CTAG
BlpI 1038 GCTCAGC GC^T[ACGT]AGC
1776 GCTCAGC
Bpu1102I 1038 GCTCAGC GC^T[ACGT]AGC
1776 GCTCAGC
BpuAI 27 GAAGAC GAAGAC(2/6)
98 GTCTTC
BsaWI 1079 ACCGGT [AT]^CCGG[AT]
1567 ACCGGT
BsmAI 645 GAGAC GTCTC(1/5)
1625 GTCTC
CelII 1038 GCTCAGC GC^T[ACGT]AGC
1776 GCTCAGC
DsaI 301 CCATGG C^C[GA][CT]GG
506 CCATGG
HgaI 1308 GACGC GACGC(5/10)
1849 GACGC
MaeI 178 CTAG C^TAG
244 CTAG
MluI 567 ACGCGT A^CGCGT
1367 ACGCGT
MseI 1631 TTAA T^TAA
1667 TTAA
NcoI 301 CCATGG C^CATGG
506 CCATGG
NspI 308 GCATGC [GA]CATG^[CT]
1043 GCATGC
PinAI 1079 ACCGGT A^CCGGT
1567 ACCGGT
PleI 181 GACTC GAGTC(4/5)
474 GAGTC
PpuMI 246 AGGACCT [GA]G^G[AT]CC[CT]
582 GGGACCC
SacI 777 GAGCTC GAGCT^C
1701 GAGCTC
SphI 308 GCATGC GCATG^C
1043 GCATGC
TfiI 72 GAATC G^A[AT]TC
121 GAATC
Tru9I 1631 TTAA T^TAA
1667 TTAA
Tsp509I 533 AATT ^AATT
1903 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI ApaLI Asp700I
AspHI AvaI AvaII BanI BanII
BbeI BbvI BcnI BmyI BpmI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI MaeII
MboI MboII MflI MnlI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SgrAI StuI
StyI TaqI TseI TspRI TthHB8I
XhoII XmnI