Restriction map for
pF1KB4878
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AvaI AviII AvrII BamHI BbeI
BbrPI BcgI BciVI BfaI BfrI
BglI BglII BlnI BsaAI BsaBI
BsaHI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspLU11I BspMI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstZ17I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoRI
EcoRV EcoT22I FseI FspI HaeII
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MamI
MfeI MluI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RsrII SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SwaI Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccIII 686 TCCGGA T^CCGGA
AlwNI 1237 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1404 CCTGAGG CC^T[ACGT]AGG
AspI 676 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 546 GCTGAGG CCTCAGC(-5/-2)
BclI 1310 TGATCA T^GATCA
BcnI 576 CCCGG CC^[GC]GG
BlpI 74 GCTCAGC GC^T[ACGT]AGC
BpmI 493 CTGGAG CTGGAG(16/14)
Bpu1102I 74 GCTCAGC GC^T[ACGT]AGC
BsaI 646 GGTCTC GGTCTC(1/5)
BsaWI 686 TCCGGA [AT]^CCGG[AT]
BseAI 686 TCCGGA T^CCGGA
BseRI 166 GAGGAG GAGGAG(10/8)
BsgI 514 GTGCAG GTGCAG(16/14)
BsmFI 1223 GTCCC GGGAC(10/14)
BspEI 686 TCCGGA T^CCGGA
BspHI 188 TCATGA T^CATGA
BsrBI 22 GAGCGG CCGCTC(-3/-3)
BsrDI 58 GCAATG GCAATG(2/0)
BsrFI 750 GCCGGT [GA]^CCGG[CT]
BstEII 564 GGTGACC G^GT[ACGT]ACC
BstPI 564 GGTGACC G^GT[ACGT]ACC
BstYI 880 AGATCC [GA]^GATC[CT]
Bsu36I 1404 CCTGAGG CC^T[ACGT]AGG
CelII 74 GCTCAGC GC^T[ACGT]AGC
CfoI 103 GCGC GCG^C
Cfr10I 750 GCCGGT [GA]^CCGG[CT]
Eco81I 1404 CCTGAGG CC^T[ACGT]AGG
EcoO65I 564 GGTGACC G^GT[ACGT]ACC
FbaI 1310 TGATCA T^GATCA
HgaI 192 GACGC GACGC(5/10)
HhaI 103 GCGC GCG^C
HinP1I 103 GCGC G^CGC
MflI 880 AGATCC [GA]^GATC[CT]
MroI 686 TCCGGA T^CCGGA
MspA1I 1420 CAGCTG C[AC]G^C[GT]G
NciI 576 CCCGG CC^[GC]GG
PflFI 676 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 1420 CAGCTG CAG^CTG
RcaI 188 TCATGA T^CATGA
SacI 760 GAGCTC GAGCT^C
SmlI 1260 CTTGAG C^T[CT][GA]AG
SspI 298 AATATT AAT^ATT
StuI 391 AGGCCT AGG^CCT
TaqI 613 TCGA T^CGA
Tth111I 676 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 613 TCGA T^CGA
XcmI 161 CCAAGGAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 880 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcsI 552 GAATTT [GA]^AATT[CT]
616 AAATTT
ApoI 552 GAATTT [GA]^AATT[CT]
616 AAATTT
AvaII 227 GGACC G^G[AT]CC
1409 GGACC
BanI 1298 GGCACC G^G[CT][GA]CC
1339 GGTGCC
BanII 760 GAGCTC G[GA]GC[CT]^C
928 GGGCTC
BsmAI 674 GAGAC GTCTC(1/5)
647 GTCTC
DraII 226 AGGACCC [GA]G^G[ACGT]CC[CT]
1408 AGGACCC
EarI 784 GAAGAG CTCTTC(1/4)
1494 GAAGAG
EcoO109I 226 AGGACCC [GA]G^G[ACGT]CC[CT]
1408 AGGACCC
Ksp632I 784 GAAGAG CTCTTC(1/4)
1494 GAAGAG
MaeII 521 ACGT A^CGT
716 ACGT
MseI 296 TTAA T^TAA
1046 TTAA
NspI 248 GCATGC [GA]CATG^[CT]
692 GCATGC
PpuMI 226 AGGACCC [GA]G^G[AT]CC[CT]
1408 AGGACCC
SexAI 968 ACCTGGT A^CC[AT]GGT
1235 ACCAGGT
SfcI 387 CTACAG C^T[GA][CT]AG
1274 CTGTAG
SphI 248 GCATGC GCATG^C
692 GCATGC
Tru9I 296 TTAA T^TAA
1046 TTAA
Tsp509I 553 AATT ^AATT
617 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
BalI BbsI BbvI BmyI BpuAI
BsaJI BsiHKAI BsiYI BslI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
Cac8I Cfr13I DdeI DpnII DsaI
EaeI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeIII MboI MboII
MluNI MnlI MscI MslI MspI
MvaI MwoI NcoI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TfiI TseI Tsp45I
TspRI