Restriction map for
pF1KB4677
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# NO CUT ENZYMES ( 100 enzymes) #
##################################
AccIII AclI AflII AgeI ApaI
AscI AspI AvaI AviII AvrII
BamHI BbeI BbrPI BbvCI BciVI
BclI BcnI BfrI BglI BlnI
BsaAI BsaWI BseAI BsgI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BsrBI BsrDI BsrGI BssHII BssSI
BstBI BstEII BstPI ClaI CpoI
DraIII DrdI DsaI EagI EclXI
Eco52I EcoO65I EcoT22I FauI FbaI
FseI FspI HpaI KasI KspI
MfeI MluI MroI MunI NarI
NciI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SphI Sse8387I SspBI StuI SwaI
Tth111I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AatII 77 GACGTC GACGT^C
Acc65I 2882 GGTACC G^GTACC
AccII 2140 CGCG CG^CG
AcyI 77 GACGTC G[GA]^CG[CT]C
AflIII 1850 ACATGT A^C[GA][CT]GT
AhdI 2029 GACATCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 289 GTGCAC G^TGCAC
AlwNI 2203 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 612 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2862 AGCGCT AGC^GCT
ApaLI 289 GTGCAC G^TGCAC
Asp718I 2882 GGTACC G^GTACC
AspEI 2029 GACATCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 269 TGGCCA TGG^CCA
BanI 2882 GGTACC G^G[CT][GA]CC
BanII 793 GAGCTC G[GA]GC[CT]^C
BbsI 2037 GTCTTC GAAGAC(2/6)
BcgI 1171 CGAGGATTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 2519 AGATCT A^GATCT
BlpI 300 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 300 GCTTAGC GC^T[ACGT]AGC
BpuAI 2037 GTCTTC GAAGAC(2/6)
BsaHI 77 GACGTC G[GA]^CG[CT]C
BsaI 978 GAGACC GGTCTC(1/5)
BsiEI 261 CGATCG CG[GA][CT]^CG
BspLU11I 1850 ACATGT A^CATGT
BspMI 866 ACCTGC ACCTGC(4/8)
BsrFI 781 GCCGGC [GA]^CCGG[CT]
BstUI 2140 CGCG CG^CG
Bsu36I 612 CCTGAGG CC^T[ACGT]AGG
Cac8I 781 GCCGGC GC[ACGT]^[ACGT]GC
CelII 300 GCTTAGC GC^T[ACGT]AGC
CfoI 2863 GCGC GCG^C
Cfr10I 781 GCCGGC [GA]^CCGG[CT]
EaeI 269 TGGCCA [CT]^GGCC[GA]
Eam1105I 2029 GACATCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2862 AGCGCT AGC^GCT
Eco81I 612 CCTGAGG CC^T[ACGT]AGG
EcoNI 1198 CCTGTGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 2693 GATATC GAT^ATC
HaeII 2862 AGCGCT [GA]GCGC^[CT]
HapII 782 CCGG C^CGG
HgaI 1145 GACGC GACGC(5/10)
HhaI 2863 GCGC GCG^C
Hin1I 77 GACGTC G[GA]^CG[CT]C
HinP1I 2863 GCGC G^CGC
HpaII 782 CCGG C^CGG
KpnI 2882 GGTACC GGTAC^C
MluNI 269 TGGCCA TGG^CCA
MscI 269 TGGCCA TGG^CCA
MspI 782 CCGG C^CGG
MvnI 2140 CGCG CG^CG
NaeI 781 GCCGGC GCC^GGC
NgoMIV 781 GCCGGC G^CCGGC
PflMI 2585 CCAAACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1194 GGGACCT [GA]G^G[AT]CC[CT]
PvuI 261 CGATCG CGAT^CG
SacI 793 GAGCTC GAGCT^C
SapI 1694 GCTCTTC GCTCTTC(1/4)
SpeI 900 ACTAGT A^CTAGT
Van91I 2585 CCAAACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AvaII 1005 GGACC G^G[AT]CC
1195 GGACC
BsaBI 13 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
58 GATCCTGATC
BseRI 1099 GAGGAG GAGGAG(10/8)
1921 GAGGAG
BsmI 2853 GAATGC GAATGC(1/-1)
2128 GCATTC
BspHI 483 TCATGA T^CATGA
1727 TCATGA
Bst1107I 2435 GTATAC GTA^TAC
2580 GTATAC
BstXI 370 CCACAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2074 CCAGTGCTTTGG
BstZ17I 2435 GTATAC GTA^TAC
2580 GTATAC
DraII 1194 GGGACCT [GA]G^G[ACGT]CC[CT]
1599 AGGGCCT
EarI 1695 CTCTTC CTCTTC(1/4)
772 GAAGAG
EcoO109I 1194 GGGACCT [GA]G^G[ACGT]CC[CT]
1599 AGGGCCT
EcoRI 853 GAATTC G^AATTC
2337 GAATTC
EcoT14I 2235 CCAAGG C^C[AT][AT]GG
2877 CCAAGG
HincII 1264 GTCAAC GT[CT]^[GA]AC
2287 GTTGAC
HindII 1264 GTCAAC GT[CT]^[GA]AC
2287 GTTGAC
HindIII 520 AAGCTT A^AGCTT
751 AAGCTT
Ksp632I 1695 CTCTTC CTCTTC(1/4)
772 GAAGAG
MaeII 78 ACGT A^CGT
1985 ACGT
MamI 13 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
58 GATCCTGATC
NspI 1850 ACATGT [GA]CATG^[CT]
2270 ACATGC
PleI 2595 GACTC GAGTC(4/5)
443 GAGTC
RcaI 483 TCATGA T^CATGA
1727 TCATGA
StyI 2235 CCAAGG C^C[AT][AT]GG
2877 CCAAGG
TaqI 264 TCGA T^CGA
1507 TCGA
TthHB8I 264 TCGA T^CGA
1507 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AseI AsnI Asp700I
AspHI BbvI BfaI BmyI BpmI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstAPI
BstF5I BstNI BstYI Cfr13I DdeI
DpnII DraI EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MvaI MwoI
NdeII NlaIII NlaIV PshBI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SspI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XcmI
XhoII XmnI