Restriction map for
pF1KB4666
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# NO CUT ENZYMES ( 203 enzymes) #
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AatII Acc65I AccI AccIII AciI
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AvaII AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BciVI BclI
BfrI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmFI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstNI BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I Cfr13I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRII EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HaeIII
HgaI Hin1I HincII HindII HindIII
HinfI HpaI HphI KasI KpnI
Ksp632I KspI MaeII MamI MboII
MfeI MluI MluNI MroI MscI
MseI MslI MunI MvaI MwoI
NaeI NarI NcoI NdeI NgoMIV
NlaIII NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI Sau96I SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI TfiI
Tru9I Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 24 enzymes) #
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AccII 18 CGCG CG^CG
AfaI 111 GTAC GT^AC
Aor51HI 32 AGCGCT AGC^GCT
BcgI 20 CGAGCGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaI 81 GGTCTC GGTCTC(1/5)
BsaJI 8 CCGGGG C^C[ACGT][ACGT]GG
BsmAI 82 GTCTC GTCTC(1/5)
BsmI 123 GAATGC GAATGC(1/-1)
BstF5I 135 GGATG GGATG(2/0)
BstUI 18 CGCG CG^CG
BstYI 187 AGATCC [GA]^GATC[CT]
DdeI 86 CTCAG C^T[ACGT]AG
Eco47III 32 AGCGCT AGC^GCT
FokI 135 GGATG GGATG(9/13)
MaeIII 167 GTAAC ^GT[ACGT]AC
MflI 187 AGATCC [GA]^GATC[CT]
MvnI 18 CGCG CG^CG
NheI 13 GCTAGC G^CTAGC
NlaIV 180 GGAGCC GG[ACGT]^[ACGT]CC
RsaI 111 GTAC GT^AC
ScaI 110 AGTACT AGT^ACT
SfaNI 136 GATGC GCATC(5/9)
SmlI 49 CTCAAG C^T[CT][GA]AG
XhoII 187 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwI 188 GATCC GGATC(4/5)
74 GGATC
BcnI 8 CCGGG CC^[GC]GG
177 CCGGG
BfaI 14 CTAG C^TAG
185 CTAG
BsiYI 141 CCTGCTGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
171 CCCCTTCCGGG
BslI 141 CCTGCTGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
171 CCCCTTCCGGG
BssKI 8 CCGGG ^CC[ACGT]GG
177 CCGGG
DpnII 75 GATC ^GATC
188 GATC
HaeII 22 AGCGCC [GA]GCGC^[CT]
32 AGCGCT
HapII 8 CCGG C^CGG
177 CCGG
HpaII 8 CCGG C^CGG
177 CCGG
MaeI 14 CTAG C^TAG
185 CTAG
MboI 75 GATC ^GATC
188 GATC
MspA1I 92 CAGCTG C[AC]G^C[GT]G
158 CAGCTG
MspI 8 CCGG C^CGG
177 CCGG
NciI 8 CCGGG CC^[GC]GG
177 CCGGG
NdeII 75 GATC ^GATC
188 GATC
PstI 28 CTGCAG CTGCA^G
95 CTGCAG
PvuII 92 CAGCTG CAG^CTG
158 CAGCTG
Sau3AI 75 GATC ^GATC
188 GATC
ScrFI 8 CCGGG CC^[ACGT]GG
177 CCGGG
SfcI 28 CTGCAG C^T[GA][CT]AG
95 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 10 enzymes) #
#########################################
AluI BbvI Cac8I CfoI Fnu4HI
HhaI HinP1I ItaI MnlI TseI