Restriction map for
pF1KB4619
##################################
# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII BanI
BbeI BbrPI BbsI BbvCI BcgI
BclI BfrI BglI BglII BlpI
Bpu1102I BpuAI BsaAI BsaI BsaWI
BseAI BsiWI BsmBI BsoBI BspDI
BspEI BspHI BspMI BsrFI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Cac8I CelII Cfr10I
ClaI CpoI DraIII Eam1105I EarI
Eco47III EcoO65I EcoRI EcoT22I FauI
FbaI FseI FspI HindIII HpaI
KasI KpnI Ksp632I KspI MaeII
MluI MroI MspA1I NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacII SapI
SbfI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 66 enzymes) #
###################################
AccI 858 GTCGAC GT^[AC][GT]AC
AccII 19 CGCG CG^CG
AcyI 274 GACGCC G[GA]^CG[CT]C
AflIII 292 ACATGT A^C[GA][CT]GT
AlwNI 329 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 406 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 904 CCTAGG C^CTAGG
BalI 321 TGGCCA TGG^CCA
BanII 133 GAGCTC G[GA]GC[CT]^C
BciVI 199 GGATAC GTATCC(6/5)
BcnI 10 CCCGG CC^[GC]GG
BfaI 905 CTAG C^TAG
BlnI 904 CCTAGG C^CTAGG
BsaBI 241 GATCGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 274 GACGCC G[GA]^CG[CT]C
BsgI 578 CTGCAC GTGCAG(16/14)
BsiEI 12 CGGCCG CG[GA][CT]^CG
BsmI 763 GAATGC GAATGC(1/-1)
Bsp1407I 484 TGTACA T^GTACA
BspLU11I 292 ACATGT A^CATGT
BsrBI 450 GAGCGG CCGCTC(-3/-3)
BsrDI 993 CATTGC GCAATG(2/0)
BsrGI 484 TGTACA T^GTACA
BstAPI 328 GCAGTCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 981 GGATG GGATG(2/0)
BstUI 19 CGCG CG^CG
CfoI 42 GCGC GCG^C
DraI 315 TTTAAA TTT^AAA
DraII 900 AGGTCCT [GA]G^G[ACGT]CC[CT]
DrdI 796 GACACAATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 68 CCATGG C^C[GA][CT]GG
EagI 12 CGGCCG C^GGCCG
EclXI 12 CGGCCG C^GGCCG
Eco52I 12 CGGCCG C^GGCCG
EcoNI 955 CCTGAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 900 AGGTCCT [GA]G^G[ACGT]CC[CT]
FokI 981 GGATG GGATG(9/13)
HaeII 41 GGCGCT [GA]GCGC^[CT]
HapII 11 CCGG C^CGG
HhaI 42 GCGC GCG^C
Hin1I 274 GACGCC G[GA]^CG[CT]C
HinP1I 42 GCGC G^CGC
HincII 858 GTCGAC GT[CT]^[GA]AC
HindII 858 GTCGAC GT[CT]^[GA]AC
HpaII 11 CCGG C^CGG
MaeI 905 CTAG C^TAG
MamI 241 GATCGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 800 CAATTG C^AATTG
MluNI 321 TGGCCA TGG^CCA
MscI 321 TGGCCA TGG^CCA
MslI 993 CATTGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 11 CCGG C^CGG
MunI 800 CAATTG C^AATTG
MvnI 19 CGCG CG^CG
NciI 10 CCCGG CC^[GC]GG
NcoI 68 CCATGG C^CATGG
NspI 292 ACATGT [GA]CATG^[CT]
PleI 648 GAGTC GAGTC(4/5)
PpuMI 900 AGGTCCT [GA]G^G[AT]CC[CT]
SacI 133 GAGCTC GAGCT^C
SalI 858 GTCGAC G^TCGAC
ScaI 820 AGTACT AGT^ACT
SspBI 484 TGTACA T^GTACA
StuI 907 AGGCCT AGG^CCT
TfiI 246 GAATC G^A[AT]TC
XmnI 406 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 410 AAATTC [GA]^AATT[CT]
733 AAATTT
AocI 896 CCTCAGG CC^T[ACGT]AGG
955 CCTGAGG
ApoI 410 AAATTC [GA]^AATT[CT]
733 AAATTT
AspHI 133 GAGCTC G[AT]GC[AT]^C
615 GAGCAC
AvaII 586 GGACC G^G[AT]CC
901 GGTCC
BamHI 309 GGATCC G^GATCC
828 GGATCC
BmyI 133 GAGCTC G[AGT]GC[ACT]^C
615 GAGCAC
BseRI 215 CTCCTC GAGGAG(10/8)
106 GAGGAG
BsiHKAI 133 GAGCTC G[AT]GC[AT]^C
615 GAGCAC
BsmAI 265 GTCTC GTCTC(1/5)
928 GTCTC
Bsp1286I 133 GAGCTC G[AGT]GC[ACT]^C
615 GAGCAC
Bsu36I 896 CCTCAGG CC^T[ACGT]AGG
955 CCTGAGG
Cfr13I 586 GGACC G^G[ACGT]CC
901 GGTCC
Eco81I 896 CCTCAGG CC^T[ACGT]AGG
955 CCTGAGG
EcoT14I 68 CCATGG C^C[AT][AT]GG
904 CCTAGG
HgaI 274 GACGC GACGC(5/10)
886 GCGTC
HinfI 246 GAATC G^A[ACGT]TC
648 GAGTC
Sau96I 586 GGACC G^G[ACGT]CC
901 GGTCC
StyI 68 CCATGG C^C[AT][AT]GG
904 CCTAGG
Tsp45I 632 GTGAC ^GT[GC]AC
809 GTGAC
TspRI 641 CAGTG CA[GC]TG[ACGT][ACGT]^
891 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BpmI BsaJI BsiYI BslI BsmFI
BsrI BssKI BstNI BstYI DdeI
DpnII EaeI EcoRII EcoRV Fnu4HI
HaeIII HphI ItaI MaeIII MboI
MboII MflI MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI ScrFI SfaNI TaqI Tru9I
TseI Tsp509I TthHB8I XhoII