Restriction map for
pF1KB4604
##################################
# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AgeI AhdI AocI
Aor51HI ApaI AscI AseI AsnI
Asp700I AspEI AspI AvrII BalI
BamHI BanII BbeI BbrPI BbsI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BspDI
BspEI BspHI BspMI BsrBI BssHII
BssSI Bst1107I BstBI BstUI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoT22I
FbaI FseI HgaI Hin1I HincII
HindII HindIII HpaI KasI KspI
MaeI MamI MfeI MluI MluNI
MroI MscI MseI MunI MvnI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspI StuI SwaI TfiI Tru9I
Tth111I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 734 GGTACC G^GTACC
AflIII 674 ACATGT A^C[GA][CT]GT
Alw44I 387 GTGCAC G^TGCAC
AlwNI 574 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 387 GTGCAC G^TGCAC
Asp718I 734 GGTACC G^GTACC
AvaI 650 CCCGGG C^[CT]CG[GA]G
AviII 494 TGCGCA TGC^GCA
BbvCI 55 CCTCAGC CCTCAGC(-5/-2)
BglI 34 GCCGGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseRI 190 GAGGAG GAGGAG(10/8)
BsoBI 650 CCCGGG C^[CT]CG[GA]G
BspLU11I 674 ACATGT A^CATGT
BsrFI 859 GCCGGC [GA]^CCGG[CT]
BstEII 525 GGTCACC G^GT[ACGT]ACC
BstPI 525 GGTCACC G^GT[ACGT]ACC
Cfr10I 859 GCCGGC [GA]^CCGG[CT]
EcoO65I 525 GGTCACC G^GT[ACGT]ACC
EcoRI 484 GAATTC G^AATTC
EcoRV 886 GATATC GAT^ATC
FspI 494 TGCGCA TGC^GCA
HaeII 611 AGCGCC [GA]GCGC^[CT]
KpnI 734 GGTACC GGTAC^C
MaeII 518 ACGT A^CGT
NaeI 859 GCCGGC GCC^GGC
NcoI 782 CCATGG C^CATGG
NgoMIV 859 GCCGGC G^CCGGC
PflMI 630 CCACCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 287 AGGACCC [GA]G^G[AT]CC[CT]
SfaNI 872 GCATC GCATC(5/9)
SfiI 33 GGCCGGGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 650 CCCGGG CCC^GGG
SmlI 511 CTCAAG C^T[CT][GA]AG
SphI 497 GCATGC GCATG^C
Van91I 630 CCACCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 650 CCCGGG C^CCGGG
XmaI 650 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcsI 160 GAATTT [GA]^AATT[CT]
484 GAATTC
ApoI 160 GAATTT [GA]^AATT[CT]
484 GAATTC
AspHI 387 GTGCAC G[AT]GC[AT]^C
767 GTGCTC
AvaII 288 GGACC G^G[AT]CC
714 GGACC
BanI 400 GGCACC G^G[CT][GA]CC
734 GGTACC
BpmI 394 CTGGAG CTGGAG(16/14)
616 CTGGAG
BsaI 626 GAGACC GGTCTC(1/5)
811 GAGACC
BsgI 173 GTGCAG GTGCAG(16/14)
333 GTGCAG
BsiHKAI 387 GTGCAC G[AT]GC[AT]^C
767 GTGCTC
BsmFI 503 GGGAC GGGAC(10/14)
653 GGGAC
Bsp1407I 677 TGTACA T^GTACA
690 TGTACA
BsrDI 790 CATTGC GCAATG(2/0)
338 GCAATG
BsrGI 677 TGTACA T^GTACA
690 TGTACA
BsrI 506 ACTGG ACTGG(1/-1)
695 ACTGG
BstAPI 239 GCAAGGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
573 GCAGAAGCTGC
CfoI 495 GCGC GCG^C
612 GCGC
DsaI 635 CCGTGG C^C[GA][CT]GG
782 CCATGG
EaeI 32 TGGCCG [CT]^GGCC[GA]
705 CGGCCA
EarI 360 CTCTTC CTCTTC(1/4)
187 GAAGAG
EcoT14I 44 CCTTGG C^C[AT][AT]GG
782 CCATGG
HhaI 495 GCGC GCG^C
612 GCGC
HinP1I 495 GCGC G^CGC
612 GCGC
HinfI 358 GACTC G^A[ACGT]TC
469 GACTC
Ksp632I 360 CTCTTC CTCTTC(1/4)
187 GAAGAG
MspA1I 297 CAGCTG C[AC]G^C[GT]G
709 CAGCTG
NspI 497 GCATGC [GA]CATG^[CT]
674 ACATGT
PleI 358 GACTC GAGTC(4/5)
469 GACTC
PvuII 297 CAGCTG CAG^CTG
709 CAGCTG
SspBI 677 TGTACA T^GTACA
690 TGTACA
StyI 44 CCTTGG C^C[AT][AT]GG
782 CCATGG
Tsp509I 161 AATT ^AATT
485 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BcnI BmyI BsaJI BsiYI BslI
BsmAI Bsp1286I BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII DraIII EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HpaII
HphI ItaI MaeIII MboI MboII
MflI MnlI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI TaqI
TseI Tsp45I TspRI TthHB8I XhoII