Restriction map for
pF1KB4489
##################################
# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AcyI AflII AgeI AhdI AocI
Aor51HI ApaI ApoI AscI AseI
AsnI Asp718I AspEI AspI AvaII
AviII AvrII BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BsaAI
BsaBI BsaHI BsiWI BsmBI BsmI
Bsp1407I BspHI BsrDI BsrFI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I Cfr10I CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI Hin1I HindIII HpaI KasI
KpnI KspI MamI MfeI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tsp509I Tth111I Van91I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccIII 1241 TCCGGA T^CCGGA
AvaI 183 CCCGGG C^[CT]CG[GA]G
BbsI 1150 GTCTTC GAAGAC(2/6)
BpuAI 1150 GTCTTC GAAGAC(2/6)
BsaI 712 GAGACC GGTCTC(1/5)
BsaWI 1241 TCCGGA [AT]^CCGG[AT]
BseAI 1241 TCCGGA T^CCGGA
BseRI 802 GAGGAG GAGGAG(10/8)
BsiEI 824 CGGTCG CG[GA][CT]^CG
BsoBI 183 CCCGGG C^[CT]CG[GA]G
BspDI 765 ATCGAT AT^CGAT
BspEI 1241 TCCGGA T^CCGGA
BspLU11I 753 ACATGT A^CATGT
BspMI 237 GCAGGT ACCTGC(4/8)
BsrBI 216 CCGCTC CCGCTC(-3/-3)
BstAPI 1298 GCACCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1333 CCACTGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 765 ATCGAT AT^CGAT
DraII 254 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 254 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 152 CCATGG C^C[AT][AT]GG
HgaI 203 GCGTC GACGC(5/10)
HincII 670 GTTGAC GT[CT]^[GA]AC
HindII 670 GTTGAC GT[CT]^[GA]AC
MaeII 1019 ACGT A^CGT
MluI 584 ACGCGT A^CGCGT
MroI 1241 TCCGGA T^CCGGA
NcoI 152 CCATGG C^CATGG
NspI 753 ACATGT [GA]CATG^[CT]
SapI 1164 GCTCTTC GCTCTTC(1/4)
ScaI 411 AGTACT AGT^ACT
SmaI 183 CCCGGG CCC^GGG
SmlI 1144 CTCAAG C^T[CT][GA]AG
StyI 152 CCATGG C^C[AT][AT]GG
XcmI 152 CCATGGACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 183 CCCGGG C^CCGGG
XmaI 183 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccII 280 CGCG CG^CG
585 CGCG
AflIII 584 ACGCGT A^C[GA][CT]GT
753 ACATGT
Alw44I 603 GTGCAC G^TGCAC
1006 GTGCAC
AlwI 1125 GATCC GGATC(4/5)
297 GGATC
ApaLI 603 GTGCAC G^TGCAC
1006 GTGCAC
Asp700I 632 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
924 GAACAGATTC
BalI 470 TGGCCA TGG^CCA
723 TGGCCA
BfaI 64 CTAG C^TAG
121 CTAG
BlpI 459 GCTCAGC GC^T[ACGT]AGC
1171 GCTCAGC
Bpu1102I 459 GCTCAGC GC^T[ACGT]AGC
1171 GCTCAGC
BssSI 313 CACGAG CACGAG(-5/-1)
427 CTCGTG
BstF5I 1244 GGATG GGATG(2/0)
1287 GGATG
BstUI 280 CGCG CG^CG
585 CGCG
BstYI 297 GGATCT [GA]^GATC[CT]
1124 AGATCC
CelII 459 GCTCAGC GC^T[ACGT]AGC
1171 GCTCAGC
CfoI 288 GCGC GCG^C
485 GCGC
DpnII 298 GATC ^GATC
1125 GATC
DsaI 152 CCATGG C^C[GA][CT]GG
1197 CCACGG
FauI 46 CCCGC CCCGC(4/6)
215 CCCGC
FokI 1244 GGATG GGATG(9/13)
1287 GGATG
HaeII 287 GGCGCT [GA]GCGC^[CT]
484 AGCGCC
HhaI 288 GCGC GCG^C
485 GCGC
HinP1I 288 GCGC G^CGC
485 GCGC
MaeI 64 CTAG C^TAG
121 CTAG
MboI 298 GATC ^GATC
1125 GATC
MflI 297 GGATCT [GA]^GATC[CT]
1124 AGATCC
MluNI 470 TGGCCA TGG^CCA
723 TGGCCA
MscI 470 TGGCCA TGG^CCA
723 TGGCCA
MvnI 280 CGCG CG^CG
585 CGCG
NdeII 298 GATC ^GATC
1125 GATC
PvuII 177 CAGCTG CAG^CTG
743 CAGCTG
Sau3AI 298 GATC ^GATC
1125 GATC
SfaNI 736 GATGC GCATC(5/9)
1061 GCATC
TaqI 766 TCGA T^CGA
786 TCGA
TthHB8I 766 TCGA T^CGA
786 TCGA
XhoII 297 GGATCT [GA]^GATC[CT]
1124 AGATCC
XmnI 632 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
924 GAACAGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
BanII BbvI BcnI BmyI BpmI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstNI Cac8I Cfr13I DdeI EaeI
EarI EcoRII Fnu4HI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeIII MboII MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NlaIII NlaIV PleI PstI RsaI
Sau96I ScrFI SfcI TfiI Tru9I
TseI Tsp45I TspRI