Restriction map for
pF1KB4426
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccIII AcsI AflII
AflIII AgeI AhdI AocI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AvaI AviII AvrII BalI
BamHI BbeI BbrPI BbsI BbvCI
BciVI BclI BfaI BfrI BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaWI BseAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI Eam1105I EarI
Eco81I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FspI HindIII HpaI
KasI KpnI Ksp632I MaeI MamI
MluI MluNI MroI MscI MseI
MslI NarI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshBI PvuI PvuII
RcaI RsrII SalI SapI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
SspBI SspI StuI StyI SwaI
Tru9I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccI 205 GTCTAC GT^[AC][GT]AC
AclI 808 AACGTT AA^CGTT
AcyI 202 GGCGTC G[GA]^CG[CT]C
Alw44I 73 GTGCAC G^TGCAC
AlwI 527 GGATC GGATC(4/5)
Aor51HI 215 AGCGCT AGC^GCT
ApaI 567 GGGCCC GGGCC^C
ApaLI 73 GTGCAC G^TGCAC
AspI 480 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 627 GGTGCC G^G[CT][GA]CC
BcgI 507 CGAGAACACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 122 GCCCCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 404 AGATCT A^GATCT
BsaHI 202 GGCGTC G[GA]^CG[CT]C
BsaI 388 GAGACC GGTCTC(1/5)
BsiEI 285 CGGCCG CG[GA][CT]^CG
BsmFI 776 GGGAC GGGAC(10/14)
BsrFI 48 GCCGGC [GA]^CCGG[CT]
BsrI 483 CCAGT ACTGG(1/-1)
BssSI 355 CACGAG CACGAG(-5/-1)
BstF5I 743 GGATG GGATG(2/0)
BstXI 119 CCAGCCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 404 AGATCT [GA]^GATC[CT]
Cfr10I 48 GCCGGC [GA]^CCGG[CT]
EagI 285 CGGCCG C^GGCCG
EclXI 285 CGGCCG C^GGCCG
Eco47III 215 AGCGCT AGC^GCT
Eco52I 285 CGGCCG C^GGCCG
EcoNI 180 CCTGGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI 743 GGATG GGATG(9/13)
FseI 47 GGCCGGCC GGCCGG^CC
HaeII 215 AGCGCT [GA]GCGC^[CT]
HgaI 203 GCGTC GACGC(5/10)
Hin1I 202 GGCGTC G[GA]^CG[CT]C
HincII 769 GTTGAC GT[CT]^[GA]AC
HindII 769 GTTGAC GT[CT]^[GA]AC
HphI 11 TCACC GGTGA(8/7)
MaeIII 656 GTGAC ^GT[ACGT]AC
MfeI 651 CAATTG C^AATTG
MflI 404 AGATCT [GA]^GATC[CT]
MunI 651 CAATTG C^AATTG
NaeI 48 GCCGGC GCC^GGC
NgoMIV 48 GCCGGC G^CCGGC
PflFI 480 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 54 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 551 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 808 AACGTT AA^CGTT
PstI 598 CTGCAG CTGCA^G
SacI 604 GAGCTC GAGCT^C
SbfI 597 CCTGCAGG CCTGCA^GG
SfcI 598 CTGCAG C^T[GA][CT]AG
Sse8387I 597 CCTGCAGG CCTGCA^GG
TfiI 668 GATTC G^A[AT]TC
Tsp45I 656 GTGAC ^GT[GC]AC
Tsp509I 652 AATT ^AATT
Tth111I 480 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 54 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 404 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AlwNI 55 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
226 CAGGGGCTG
AvaII 18 GGTCC G^G[AT]CC
462 GGACC
BspMI 172 ACCTGC ACCTGC(4/8)
644 ACCTGC
BstAPI 167 GCATGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
270 GCACGCCCTGC
DraII 566 AGGGCCC [GA]G^G[ACGT]CC[CT]
567 GGGCCCC
EcoO109I 566 AGGGCCC [GA]G^G[ACGT]CC[CT]
567 GGGCCCC
KspI 14 CCGCGG CCGC^GG
288 CCGCGG
MaeII 767 ACGT A^CGT
809 ACGT
MboII 456 GAAGA GAAGA(8/7)
407 TCTTC
PleI 343 GACTC GAGTC(4/5)
556 GAGTC
SacII 14 CCGCGG CCGC^GG
288 CCGCGG
SfaNI 428 GCATC GCATC(5/9)
716 GCATC
TaqI 335 TCGA T^CGA
797 TCGA
TthHB8I 335 TCGA T^CGA
797 TCGA
XcmI 368 CCACCTCTGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
614 CCAGCCCACGCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
BanII BbvI BcnI BmyI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BssKI BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EaeI EcoRII FauI Fnu4HI
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI MboI MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI TseI TspRI