Restriction map for
pF1KB4375
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# NO CUT ENZYMES ( 136 enzymes) #
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AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI AlwI AlwNI
AocI Aor51HI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaWI BseAI BsgI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI
BstEII BstPI BstYI Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KasI KpnI MamI
MfeI MflI MluI MluNI MroI
MscI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XhoII
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 919 GTATAC GT^[AC][GT]AC
AccII 50 CGCG CG^CG
AcyI 1042 GGCGTC G[GA]^CG[CT]C
AfaI 883 GTAC GT^AC
ApaI 86 GGGCCC GGGCC^C
BanII 86 GGGCCC G[GA]GC[CT]^C
BfaI 184 CTAG C^TAG
BsaHI 1042 GGCGTC G[GA]^CG[CT]C
BsaI 821 GGTCTC GGTCTC(1/5)
BseRI 1051 CTCCTC GAGGAG(10/8)
BsmBI 1003 GAGACG CGTCTC(1/5)
BspMI 161 ACCTGC ACCTGC(4/8)
BsrDI 485 GCAATG GCAATG(2/0)
Bst1107I 919 GTATAC GTA^TAC
BstUI 50 CGCG CG^CG
BstXI 1139 CCATCAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 919 GTATAC GTA^TAC
DrdI 644 GACCACCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 49 CCGCGG C^C[GA][CT]GG
EarI 124 CTCTTC CTCTTC(1/4)
EcoNI 1050 CCTCCTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 45 AGCGCC [GA]GCGC^[CT]
HgaI 1043 GCGTC GACGC(5/10)
Hin1I 1042 GGCGTC G[GA]^CG[CT]C
Ksp632I 124 CTCTTC CTCTTC(1/4)
KspI 49 CCGCGG CCGC^GG
MaeI 184 CTAG C^TAG
MvnI 50 CGCG CG^CG
NlaIII 1110 CATG CATG^
PaeR7I 728 CTCGAG C^TCGAG
RsaI 883 GTAC GT^AC
SacII 49 CCGCGG CCGC^GG
SapI 123 GCTCTTC GCTCTTC(1/4)
SexAI 648 ACCTGGT A^CC[AT]GGT
SmaI 95 CCCGGG CCC^GGG
TfiI 612 GATTC G^A[AT]TC
XhoI 728 CTCGAG C^TCGAG
XmaCI 95 CCCGGG C^CCGGG
XmaI 95 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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Alw44I 987 GTGCAC G^TGCAC
1087 GTGCAC
ApaLI 987 GTGCAC G^TGCAC
1087 GTGCAC
Asp700I 408 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
972 GAAGCCCTTC
BglI 230 GCCTTGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
275 GCCTCGGTGGC
BsmAI 1003 GAGAC GTCTC(1/5)
822 GTCTC
DdeI 268 CTAAG C^T[ACGT]AG
332 CTAAG
DpnII 793 GATC ^GATC
1071 GATC
EcoRI 602 GAATTC G^AATTC
620 GAATTC
MaeIII 311 GTCAC ^GT[ACGT]AC
327 GTCAC
MboI 793 GATC ^GATC
1071 GATC
MseI 509 TTAA T^TAA
851 TTAA
MslI 828 CATCACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1104 CATCAACATG
NdeII 793 GATC ^GATC
1071 GATC
PleI 376 GACTC GAGTC(4/5)
223 GAGTC
PstI 38 CTGCAG CTGCA^G
982 CTGCAG
PvuII 114 CAGCTG CAG^CTG
743 CAGCTG
Sau3AI 793 GATC ^GATC
1071 GATC
Tru9I 509 TTAA T^TAA
851 TTAA
Tsp45I 311 GTCAC ^GT[GC]AC
327 GTCAC
TspRI 936 CACTG CA[GC]TG[ACGT][ACGT]^
985 CAGTG
XcmI 693 CCAGGGACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
947 CCAGCTATGAGGTGG
XmnI 408 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
972 GAAGCCCTTC
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# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AcsI AluI ApoI AspHI
AvaI AvaII BanI BbvI BcnI
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DraII EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MboII MnlI MspA1I
MspI MvaI MwoI NciI NlaIV
PpuMI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TseI Tsp509I
TthHB8I