Restriction map for
pF1KB4343
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI AocI Aor51HI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaWI BseAI
BseRI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoT22I FbaI FseI FspI HaeII
HhaI HinP1I HincII HindII HindIII
HpaI HphI KasI KpnI MfeI
MflI MluI MluNI MroI MscI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tth111I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 598 GTAGAC GT^[AC][GT]AC
AccII 11 CGCG CG^CG
AcyI 448 GGCGTC G[GA]^CG[CT]C
AlwI 372 GGATC GGATC(4/5)
AlwNI 19 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 5 GGGCCC GGGCC^C
AvaII 97 GGACC G^G[AT]CC
BbvCI 288 CCTCAGC CCTCAGC(-5/-2)
BcnI 896 CCGGG CC^[GC]GG
BsaBI 400 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 448 GGCGTC G[GA]^CG[CT]C
BsmFI 96 GGGAC GGGAC(10/14)
BsmI 579 GAATGC GAATGC(1/-1)
BspMI 247 ACCTGC ACCTGC(4/8)
BstUI 11 CGCG CG^CG
DraII 5 GGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 1002 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 10 CCGCGG C^C[GA][CT]GG
EcoNI 316 CCTATTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 5 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRI 1055 GAATTC G^AATTC
EcoRV 89 GATATC GAT^ATC
EcoT14I 53 CCAAGG C^C[AT][AT]GG
HapII 896 CCGG C^CGG
HgaI 449 GCGTC GACGC(5/10)
Hin1I 448 GGCGTC G[GA]^CG[CT]C
HpaII 896 CCGG C^CGG
KspI 10 CCGCGG CCGC^GG
MaeII 646 ACGT A^CGT
MamI 400 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 740 CACAGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 10 CCGCGG C[AC]G^C[GT]G
MspI 896 CCGG C^CGG
MvnI 11 CGCG CG^CG
NciI 896 CCGGG CC^[GC]GG
NlaIII 331 CATG CATG^
PflMI 628 CCAGGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 862 GAGTC GAGTC(4/5)
SacI 1036 GAGCTC GAGCT^C
SacII 10 CCGCGG CCGC^GG
SfaNI 189 GATGC GCATC(5/9)
SmlI 160 CTTGAG C^T[CT][GA]AG
SspI 549 AATATT AAT^ATT
StyI 53 CCAAGG C^C[AT][AT]GG
TaqI 110 TCGA T^CGA
TthHB8I 110 TCGA T^CGA
Van91I 628 CCAGGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 523 CCAATAAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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AcsI 605 GAATTT [GA]^AATT[CT]
1055 GAATTC
AfaI 470 GTAC GT^AC
887 GTAC
Alw44I 659 GTGCAC G^TGCAC
999 GTGCAC
ApaLI 659 GTGCAC G^TGCAC
999 GTGCAC
ApoI 605 GAATTT [GA]^AATT[CT]
1055 GAATTC
BanII 5 GGGCCC G[GA]GC[CT]^C
1036 GAGCTC
BsaI 516 GAGACC GGTCTC(1/5)
213 GGTCTC
BsrI 24 ACTGG ACTGG(1/-1)
100 CCAGT
DpnII 373 GATC ^GATC
430 GATC
EarI 76 GAAGAG CTCTTC(1/4)
147 GAAGAG
FauI 9 CCCGC CCCGC(4/6)
12 GCGGG
Ksp632I 76 GAAGAG CTCTTC(1/4)
147 GAAGAG
MboI 373 GATC ^GATC
430 GATC
MseI 671 TTAA T^TAA
1043 TTAA
NdeII 373 GATC ^GATC
430 GATC
RsaI 470 GTAC GT^AC
887 GTAC
Sau3AI 373 GATC ^GATC
430 GATC
Tru9I 671 TTAA T^TAA
1043 TTAA
Tsp509I 606 AATT ^AATT
1056 AATT
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AluI AspHI BbvI BfaI
BmyI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BssKI BstF5I
BstNI Cac8I Cfr13I DdeI EcoRII
Fnu4HI FokI HaeIII HinfI ItaI
MaeI MaeIII MboII MnlI MvaI
MwoI NlaIV Sau96I ScrFI TfiI
TseI Tsp45I TspRI