Restriction map for
pF1KB4244
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# NO CUT ENZYMES ( 162 enzymes) #
##################################
Acc65I AccI AccII AccIII AciI
AclI AflII AflIII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspEI AspHI
AspI AvaII AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
CelII CfoI Cfr10I Cfr13I ClaI
CpoI DraI DraII DraIII EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI HinP1I HincII HindII
HindIII HpaI HphI KasI KpnI
KspI MamI MluI MluNI MroI
MscI MslI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI Sau96I SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SphI Sse8387I SspBI StuI SwaI
TaqI Tth111I TthHB8I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 115 GACGTC GACGT^C
AcyI 115 GACGTC G[GA]^CG[CT]C
AfaI 223 GTAC GT^AC
AocI 576 CCTTAGG CC^T[ACGT]AGG
AseI 757 ATTAAT AT^TAAT
AsnI 757 ATTAAT AT^TAAT
AvaI 175 CCCGGG C^[CT]CG[GA]G
BanII 384 GAGCCC G[GA]GC[CT]^C
BbsI 6 GAAGAC GAAGAC(2/6)
BfaI 479 CTAG C^TAG
BmyI 384 GAGCCC G[AGT]GC[ACT]^C
BpuAI 6 GAAGAC GAAGAC(2/6)
BsaHI 115 GACGTC G[GA]^CG[CT]C
BsmFI 172 GTCCC GGGAC(10/14)
BsoBI 175 CCCGGG C^[CT]CG[GA]G
Bsp1286I 384 GAGCCC G[AGT]GC[ACT]^C
BsrDI 656 CATTGC GCAATG(2/0)
Bsu36I 576 CCTTAGG CC^T[ACGT]AGG
DrdI 719 GACAGCCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 650 CCATGG C^C[GA][CT]GG
Eco81I 576 CCTTAGG CC^T[ACGT]AGG
EcoRI 415 GAATTC G^AATTC
EcoT14I 650 CCATGG C^C[AT][AT]GG
HaeIII 609 GGCC GG^CC
HapII 176 CCGG C^CGG
Hin1I 115 GACGTC G[GA]^CG[CT]C
HpaII 176 CCGG C^CGG
MaeI 479 CTAG C^TAG
MaeII 116 ACGT A^CGT
MfeI 226 CAATTG C^AATTG
MspA1I 43 CAGCTG C[AC]G^C[GT]G
MspI 176 CCGG C^CGG
MunI 226 CAATTG C^AATTG
NcoI 650 CCATGG C^CATGG
NlaIV 697 GGAGCC GG[ACGT]^[ACGT]CC
PshBI 757 ATTAAT AT^TAAT
PstI 84 CTGCAG CTGCA^G
PvuII 43 CAGCTG CAG^CTG
RsaI 223 GTAC GT^AC
SfcI 84 CTGCAG C^T[GA][CT]AG
SmaI 175 CCCGGG CCC^GGG
SmlI 282 CTTGAG C^T[CT][GA]AG
SpeI 478 ACTAGT A^CTAGT
SspI 268 AATATT AAT^ATT
StyI 650 CCATGG C^C[AT][AT]GG
XcmI 193 CCAAGTCATTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 175 CCCGGG C^CCGGG
XmaI 175 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcsI 415 GAATTC [GA]^AATT[CT]
425 GAATTT
ApoI 415 GAATTC [GA]^AATT[CT]
425 GAATTT
BcnI 175 CCCGG CC^[GC]GG
176 CCGGG
BpmI 630 CTCCAG CTGGAG(16/14)
623 CTGGAG
BsmAI 626 GAGAC GTCTC(1/5)
717 GAGAC
BsrI 641 ACTGG ACTGG(1/-1)
707 ACTGG
BstNI 395 CCAGG CC^[AT]GG
622 CCTGG
Cac8I 568 GCCAGC GC[ACGT]^[ACGT]GC
660 GCCAGC
EarI 714 GAAGAG CTCTTC(1/4)
763 GAAGAG
EcoRII 395 CCAGG ^CC[AT]GG
622 CCTGG
Ksp632I 714 GAAGAG CTCTTC(1/4)
763 GAAGAG
MvaI 395 CCAGG CC^[AT]GG
622 CCTGG
NciI 175 CCCGG CC^[GC]GG
176 CCGGG
NlaIII 153 CATG CATG^
651 CATG
PleI 628 GACTC GAGTC(4/5)
527 GAGTC
SfaNI 445 GATGC GCATC(5/9)
469 GCATC
TspRI 531 CAGTG CA[GC]TG[ACGT][ACGT]^
640 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AluI AlwI BbvI BsaJI BsiYI
BslI BssKI BstF5I BstYI DdeI
DpnII Fnu4HI FokI HinfI ItaI
MaeIII MboI MboII MflI MnlI
MseI MwoI NdeII Sau3AI ScrFI
TfiI Tru9I TseI Tsp45I Tsp509I
XhoII