Restriction map for
pF1KB4216
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# NO CUT ENZYMES ( 73 enzymes) #
##################################
AccIII AclI AflII AgeI Alw44I
ApaLI AscI AseI AsnI BbeI
BciVI BfrI BlpI Bpu1102I BseAI
BsiWI BspDI BspEI BspHI BspLU11I
BssHII BssSI Bst1107I BstBI BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI EagI EclXI Eco52I FseI
HincII HindII HpaI KasI KspI
MfeI MroI MslI MunI NarI
NdeI NheI NotI NruI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SfiI SfoI
SfuI SgrAI SnaBI SpeI SspI
SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AatII 2631 GACGTC GACGT^C
Acc65I 945 GGTACC G^GTACC
AccI 1429 GTAGAC GT^[AC][GT]AC
AcsI 2497 GAATTC [GA]^AATT[CT]
AflIII 3005 ACGCGT A^C[GA][CT]GT
AhdI 1309 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2452 CCTCAGG CC^T[ACGT]AGG
Aor51HI 2952 AGCGCT AGC^GCT
ApaI 1514 GGGCCC GGGCC^C
ApoI 2497 GAATTC [GA]^AATT[CT]
Asp718I 945 GGTACC G^GTACC
AspEI 1309 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1063 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1224 CCTAGG C^CTAGG
BamHI 2053 GGATCC G^GATCC
BbrPI 2905 CACGTG CAC^GTG
BbvCI 2217 CCTCAGC CCTCAGC(-5/-2)
BcgI 2247 CGAGCACAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 452 TGATCA T^GATCA
BglII 1743 AGATCT A^GATCT
BlnI 1224 CCTAGG C^CTAGG
BsaAI 2905 CACGTG [CT]AC^GT[GA]
BsaBI 1744 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1730 ACCGGA [AT]^CCGG[AT]
BsiEI 3351 CGACCG CG[GA][CT]^CG
BsmBI 2669 GAGACG CGTCTC(1/5)
BsmI 516 GAATGC GAATGC(1/-1)
Bsp1407I 2070 TGTACA T^GTACA
BsrGI 2070 TGTACA T^GTACA
Bsu36I 2452 CCTCAGG CC^T[ACGT]AGG
Eam1105I 1309 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2952 AGCGCT AGC^GCT
Eco81I 2452 CCTCAGG CC^T[ACGT]AGG
EcoRI 2497 GAATTC G^AATTC
EcoRV 1022 GATATC GAT^ATC
EcoT22I 2779 ATGCAT ATGCA^T
FbaI 452 TGATCA T^GATCA
KpnI 945 GGTACC GGTAC^C
MamI 1744 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 3005 ACGCGT A^CGCGT
NsiI 2779 ATGCAT ATGCA^T
NspI 2781 GCATGC [GA]CATG^[CT]
PflFI 1063 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2905 CACGTG CAC^GTG
PmlI 2905 CACGTG CAC^GTG
SacI 2580 GAGCTC GAGCT^C
SbfI 2047 CCTGCAGG CCTGCA^GG
ScaI 2516 AGTACT AGT^ACT
SexAI 1064 ACCTGGT A^CC[AT]GGT
SphI 2781 GCATGC GCATG^C
Sse8387I 2047 CCTGCAGG CCTGCA^GG
SspBI 2070 TGTACA T^GTACA
StuI 2402 AGGCCT AGG^CCT
Tth111I 1063 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 1703 CGCG CG^CG
3006 CGCG
AcyI 1081 GACGCC G[GA]^CG[CT]C
2631 GACGTC
Asp700I 1917 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2301 GAAGTTTTTC
AviII 2388 TGCGCA TGC^GCA
3053 TGCGCA
BanI 945 GGTACC G^G[CT][GA]CC
1727 GGCACC
BfaI 527 CTAG C^TAG
1225 CTAG
BsaHI 1081 GACGCC G[GA]^CG[CT]C
2631 GACGTC
BstEII 588 GGTCACC G^GT[ACGT]ACC
1713 GGTCACC
BstPI 588 GGTCACC G^GT[ACGT]ACC
1713 GGTCACC
BstUI 1703 CGCG CG^CG
3006 CGCG
DsaI 35 CCATGG C^C[GA][CT]GG
2162 CCATGG
EarI 1613 CTCTTC CTCTTC(1/4)
2485 GAAGAG
EcoO65I 588 GGTCACC G^GT[ACGT]ACC
1713 GGTCACC
FspI 2388 TGCGCA TGC^GCA
3053 TGCGCA
Hin1I 1081 GACGCC G[GA]^CG[CT]C
2631 GACGTC
HindIII 1204 AAGCTT A^AGCTT
2041 AAGCTT
Ksp632I 1613 CTCTTC CTCTTC(1/4)
2485 GAAGAG
MaeI 527 CTAG C^TAG
1225 CTAG
MvnI 1703 CGCG CG^CG
3006 CGCG
NaeI 1780 GCCGGC GCC^GGC
1931 GCCGGC
NcoI 35 CCATGG C^CATGG
2162 CCATGG
NgoMIV 1780 GCCGGC G^CCGGC
1931 GCCGGC
SmaI 1988 CCCGGG CCC^GGG
3294 CCCGGG
TfiI 751 GATTC G^A[AT]TC
1443 GATTC
XmaCI 1988 CCCGGG C^CCGGG
3294 CCCGGG
XmaI 1988 CCCGGG C^CCGGG
3294 CCCGGG
XmnI 1917 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2301 GAAGTTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BalI BanII
BbsI BbvI BcnI BglI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII EaeI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII