Restriction map for
pF1KB4062
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# NO CUT ENZYMES ( 145 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI Alw44I
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspHI
AspI AvaI AviII AvrII BalI
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaWI BseAI
BseRI BsiHKAI BsiWI BsmI BsoBI
BspEI BspHI BspLU11I BsrDI BssHII
Bst1107I BstBI BstXI BstZ17I CelII
CpoI DraI DraIII DrdI DsaI
EarI Eco47III EcoNI EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HindIII KasI KpnI Ksp632I
KspI MaeI MamI MfeI MluI
MluNI MroI MscI MslI MspA1I
MunI NarI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tsp509I Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AcyI 340 GACGCC G[GA]^CG[CT]C
AfaI 90 GTAC GT^AC
AhdI 240 GACCCAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 470 CCTCAGG CC^T[ACGT]AGG
AspEI 240 GACCCAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 44 GGATCC G^GATCC
BanII 493 GGGCTC G[GA]GC[CT]^C
BcnI 425 CCCGG CC^[GC]GG
BglI 213 GCCGCTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 493 GGGCTC G[AGT]GC[ACT]^C
BsaHI 340 GACGCC G[GA]^CG[CT]C
BsaI 247 GGTCTC GGTCTC(1/5)
BsaJI 243 CCAAGG C^C[ACGT][ACGT]GG
BsmBI 148 GAGACG CGTCTC(1/5)
Bsp1286I 493 GGGCTC G[AGT]GC[ACT]^C
Bsp1407I 89 TGTACA T^GTACA
BspDI 286 ATCGAT AT^CGAT
BspMI 374 ACCTGC ACCTGC(4/8)
BsrBI 214 CCGCTC CCGCTC(-3/-3)
BsrFI 70 GCCGGC [GA]^CCGG[CT]
BsrGI 89 TGTACA T^GTACA
BssSI 397 CACGAG CACGAG(-5/-1)
BstAPI 369 GCAGGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 114 GGTGACC G^GT[ACGT]ACC
BstF5I 186 GGATG GGATG(2/0)
BstPI 114 GGTGACC G^GT[ACGT]ACC
Bsu36I 470 CCTCAGG CC^T[ACGT]AGG
CfoI 269 GCGC GCG^C
Cfr10I 70 GCCGGC [GA]^CCGG[CT]
ClaI 286 ATCGAT AT^CGAT
DdeI 471 CTCAG C^T[ACGT]AG
Eam1105I 240 GACCCAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 470 CCTCAGG CC^T[ACGT]AGG
EcoO65I 114 GGTGACC G^GT[ACGT]ACC
EcoT14I 243 CCAAGG C^C[AT][AT]GG
FokI 186 GGATG GGATG(9/13)
HhaI 269 GCGC GCG^C
Hin1I 340 GACGCC G[GA]^CG[CT]C
HinP1I 269 GCGC G^CGC
HincII 504 GTTAAC GT[CT]^[GA]AC
HindII 504 GTTAAC GT[CT]^[GA]AC
HpaI 504 GTTAAC GTT^AAC
MseI 505 TTAA T^TAA
NaeI 70 GCCGGC GCC^GGC
NciI 425 CCCGG CC^[GC]GG
NgoMIV 70 GCCGGC G^CCGGC
PleI 482 GACTC GAGTC(4/5)
RsaI 90 GTAC GT^AC
SspBI 89 TGTACA T^GTACA
StyI 243 CCAAGG C^C[AT][AT]GG
TaqI 287 TCGA T^CGA
TfiI 429 GATTC G^A[AT]TC
Tru9I 505 TTAA T^TAA
TthHB8I 287 TCGA T^CGA
XcmI 122 CCATTCCCACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccII 66 CGCG CG^CG
277 CGCG
AluI 101 AGCT AG^CT
305 AGCT
AvaII 239 GGACC G^G[AT]CC
372 GGACC
BbvI 453 GCAGC GCAGC(8/12)
306 GCTGC
BsgI 307 CTGCAC GTGCAG(16/14)
451 GTGCAG
BsmAI 148 GAGAC GTCTC(1/5)
248 GTCTC
BstUI 66 CGCG CG^CG
277 CGCG
Cac8I 70 GCCGGC GC[ACGT]^[ACGT]GC
295 GCTCGC
DraII 238 GGGACCC [GA]G^G[ACGT]CC[CT]
371 AGGACCT
EagI 68 CGGCCG C^GGCCG
211 CGGCCG
EclXI 68 CGGCCG C^GGCCG
211 CGGCCG
Eco52I 68 CGGCCG C^GGCCG
211 CGGCCG
EcoO109I 238 GGGACCC [GA]G^G[ACGT]CC[CT]
371 AGGACCT
HgaI 64 GACGC GACGC(5/10)
340 GACGC
HinfI 429 GATTC G^A[ACGT]TC
482 GACTC
HphI 114 GGTGA GGTGA(8/7)
153 GGTGA
MaeII 143 ACGT A^CGT
179 ACGT
MaeIII 115 GTGAC ^GT[ACGT]AC
154 GTGAC
MvnI 66 CGCG CG^CG
277 CGCG
PpuMI 238 GGGACCC [GA]G^G[AT]CC[CT]
371 AGGACCT
SfaNI 382 GATGC GCATC(5/9)
284 GCATC
TseI 306 GCTGC G^C[AT]GC
453 GCAGC
Tsp45I 115 GTGAC ^GT[GC]AC
154 GTGAC
TspRI 129 CACTG CA[GC]TG[ACGT][ACGT]^
234 CACTG
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# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AlwI AlwNI BsiEI BsiYI
BslI BsmFI BsrI BssKI BstNI
BstYI Cfr13I DpnII EaeI EcoRII
Fnu4HI HaeIII HapII HpaII ItaI
MboI MboII MflI MnlI MspI
MvaI MwoI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI XhoII