Restriction map for
pF1KB4054
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# NO CUT ENZYMES ( 194 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AvaII AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfaI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaAI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmFI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstUI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I FauI FbaI FokI
FseI FspI HaeII HapII HgaI
Hin1I HincII HindII HindIII HinfI
HpaI HpaII HphI KasI KpnI
Ksp632I KspI MaeI MflI MluI
MluNI MroI MscI MslI MspA1I
MspI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StyI SwaI TaqI TfiI
Tth111I TthHB8I Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AccI 47 GTAGAC GT^[AC][GT]AC
AluI 80 AGCT AG^CT
AlwI 260 GGATC GGATC(4/5)
ApaI 92 GGGCCC GGGCC^C
BbsI 346 GAAGAC GAAGAC(2/6)
BpuAI 346 GAAGAC GAAGAC(2/6)
BsaBI 255 GATATGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 88 CCTGGG C^C[ACGT][ACGT]GG
BsiYI 354 CCCACGAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 354 CCCACGAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 287 GAGAC GTCTC(1/5)
BsmBI 287 GAGACG CGTCTC(1/5)
BsmI 407 GAATGC GAATGC(1/-1)
BspMI 234 ACCTGC ACCTGC(4/8)
BsrDI 208 GCAATG GCAATG(2/0)
BstXI 250 CCAGAGATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 246 GCGC GCG^C
DpnII 261 GATC ^GATC
EcoT22I 409 ATGCAT ATGCA^T
HhaI 246 GCGC GCG^C
HinP1I 246 GCGC G^CGC
MaeII 381 ACGT A^CGT
MamI 255 GATATGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 261 GATC ^GATC
MboII 346 GAAGA GAAGA(8/7)
MfeI 52 CAATTG C^AATTG
MseI 296 TTAA T^TAA
MunI 52 CAATTG C^AATTG
NdeII 261 GATC ^GATC
NsiI 409 ATGCAT ATGCA^T
PstI 236 CTGCAG CTGCA^G
Sau3AI 261 GATC ^GATC
Tru9I 296 TTAA T^TAA
TspRI 204 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 78 CCAGCTCAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 15 enzymes) #
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BanII 92 GGGCCC G[GA]GC[CT]^C
129 GAGCCC
BbvI 57 GCAGC GCAGC(8/12)
340 GCAGC
BmyI 92 GGGCCC G[AGT]GC[ACT]^C
129 GAGCCC
Bsp1286I 92 GGGCCC G[AGT]GC[ACT]^C
129 GAGCCC
Cac8I 60 GCCTGC GC[ACGT]^[ACGT]GC
225 GCAGGC
Cfr13I 92 GGGCC G^G[ACGT]CC
93 GGCCC
DraII 91 GGGGCCC [GA]G^G[ACGT]CC[CT]
92 GGGCCCC
EcoO109I 91 GGGGCCC [GA]G^G[ACGT]CC[CT]
92 GGGCCCC
NlaIII 141 CATG CATG^
219 CATG
Sau96I 92 GGGCC G^G[ACGT]CC
93 GGCCC
SfcI 106 CTACAG C^T[GA][CT]AG
236 CTGCAG
StuI 70 AGGCCT AGG^CCT
185 AGGCCT
TseI 57 GCAGC G^C[AT]GC
340 GCAGC
Tsp45I 35 GTGAC ^GT[GC]AC
149 GTGAC
Tsp509I 53 AATT ^AATT
331 AATT
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# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
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AciI BssKI BstNI DdeI EcoRII
Fnu4HI HaeIII ItaI MaeIII MnlI
MvaI MwoI NlaIV ScrFI