Restriction map for
pF1KB3938
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# NO CUT ENZYMES ( 160 enzymes) #
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AatII Acc65I AccIII AclI AfaI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BanII BbeI BbrPI BcgI
BciVI BcnI BfaI BfrI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaWI BseAI BsiWI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoT22I FseI FspI HaeII HapII
HincII HindII HindIII HpaI HpaII
KasI KpnI Ksp632I KspI MaeI
MaeII MaeIII MamI MluI MroI
MspA1I MspI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfaNI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI Tsp45I Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 28 enzymes) #
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AccI 475 GTCTAC GT^[AC][GT]AC
AcsI 1131 GAATTC [GA]^AATT[CT]
AcyI 138 GACGCC G[GA]^CG[CT]C
ApoI 1131 GAATTC [GA]^AATT[CT]
AspHI 578 GTGCTC G[AT]GC[AT]^C
BbsI 783 GAAGAC GAAGAC(2/6)
BbvCI 1020 CCTCAGC CCTCAGC(-5/-2)
BmyI 578 GTGCTC G[AGT]GC[ACT]^C
BpuAI 783 GAAGAC GAAGAC(2/6)
BsaHI 138 GACGCC G[GA]^CG[CT]C
BseRI 79 GAGGAG GAGGAG(10/8)
BsgI 445 CTGCAC GTGCAG(16/14)
BsiEI 190 CGGTCG CG[GA][CT]^CG
BsiHKAI 578 GTGCTC G[AT]GC[AT]^C
BsmBI 951 GAGACG CGTCTC(1/5)
Bsp1286I 578 GTGCTC G[AGT]GC[ACT]^C
DraI 930 TTTAAA TTT^AAA
DraII 273 AGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 551 CCGTGG C^C[GA][CT]GG
EcoO109I 273 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 1131 GAATTC G^AATTC
EcoRV 596 GATATC GAT^ATC
FauI 93 CCCGC CCCGC(4/6)
Hin1I 138 GACGCC G[GA]^CG[CT]C
MfeI 1148 CAATTG C^AATTG
MunI 1148 CAATTG C^AATTG
PleI 570 GAGTC GAGTC(4/5)
PpuMI 273 AGGACCC [GA]G^G[AT]CC[CT]
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# TWO CUT ENZYMES ( 25 enzymes) #
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BclI 936 TGATCA T^GATCA
1278 TGATCA
BglI 178 GCCAGTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
421 GCCAGCTTGGC
BsaI 437 GAGACC GGTCTC(1/5)
681 GGTCTC
BsiYI 265 CCGTGCCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
998 CCCTCTGCTGG
BslI 265 CCGTGCCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
998 CCCTCTGCTGG
BsmFI 404 GTCCC GGGAC(10/14)
700 GTCCC
BspMI 201 ACCTGC ACCTGC(4/8)
440 ACCTGC
BssKI 33 CCTGG ^CC[ACGT]GG
981 CCTGG
BstF5I 462 CATCC GGATG(2/0)
772 CATCC
BstNI 33 CCTGG CC^[AT]GG
981 CCTGG
BstYI 68 GGATCT [GA]^GATC[CT]
235 GGATCT
EcoRII 33 CCTGG ^CC[AT]GG
981 CCTGG
FbaI 936 TGATCA T^GATCA
1278 TGATCA
FokI 462 CATCC GGATG(9/13)
772 CATCC
HgaI 26 GACGC GACGC(5/10)
138 GACGC
MboII 783 GAAGA GAAGA(8/7)
1225 GAAGA
MflI 68 GGATCT [GA]^GATC[CT]
235 GGATCT
MvaI 33 CCTGG CC^[AT]GG
981 CCTGG
NspI 392 GCATGC [GA]CATG^[CT]
917 GCATGC
ScrFI 33 CCTGG CC^[ACGT]GG
981 CCTGG
SphI 392 GCATGC GCATG^C
917 GCATGC
TaqI 281 TCGA T^CGA
600 TCGA
TthHB8I 281 TCGA T^CGA
600 TCGA
XcmI 173 CCAACGCCAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
422 CCAGCTTGGCTCTGG
XhoII 68 GGATCT [GA]^GATC[CT]
235 GGATCT
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AccII AciI AluI AlwI AvaII
BalI BanI BbvI BsaJI BsmAI
BsrI BstUI Cac8I CfoI Cfr13I
DdeI DpnII EaeI EcoT14I Fnu4HI
HaeIII HhaI HinP1I HinfI HphI
ItaI MboI MluNI MnlI MscI
MseI MslI MvnI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I StyI
TfiI Tru9I TseI Tsp509I TspRI