Restriction map for
pF1KB3878
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AviII AvrII BalI BamHI
BanI BbeI BbrPI BcgI BclI
BfrI BglII BlnI BsaAI BsaBI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MslI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tsp509I
Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccII 223 CGCG CG^CG
AcyI 628 GGCGTC G[GA]^CG[CT]C
AfaI 948 GTAC GT^AC
ApaI 91 GGGCCC GGGCC^C
Asp700I 330 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 625 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 778 GGATAC GTATCC(6/5)
BfaI 1006 CTAG C^TAG
BglI 43 GCCTCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 628 GGCGTC G[GA]^CG[CT]C
BsaI 763 GAGACC GGTCTC(1/5)
BsmAI 763 GAGAC GTCTC(1/5)
BspHI 1109 TCATGA T^CATGA
BstEII 5 GGTCACC G^GT[ACGT]ACC
BstPI 5 GGTCACC G^GT[ACGT]ACC
BstUI 223 CGCG CG^CG
BstXI 1094 CCATCCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 600 GGATCT [GA]^GATC[CT]
EarI 487 GAAGAG CTCTTC(1/4)
EcoNI 279 CCTGCGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 5 GGTCACC G^GT[ACGT]ACC
HgaI 629 GCGTC GACGC(5/10)
Hin1I 628 GGCGTC G[GA]^CG[CT]C
Ksp632I 487 GAAGAG CTCTTC(1/4)
MaeI 1006 CTAG C^TAG
MaeII 1043 ACGT A^CGT
MflI 600 GGATCT [GA]^GATC[CT]
MvnI 223 CGCG CG^CG
NcoI 651 CCATGG C^CATGG
PflFI 625 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 1129 CTGCAG CTGCA^G
PvuII 523 CAGCTG CAG^CTG
RcaI 1109 TCATGA T^CATGA
RsaI 948 GTAC GT^AC
SacI 346 GAGCTC GAGCT^C
SfcI 1129 CTGCAG C^T[GA][CT]AG
SfiI 42 GGCCTCAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 658 CTCAAG C^T[CT][GA]AG
StuI 378 AGGCCT AGG^CCT
TaqI 968 TCGA T^CGA
Tsp45I 6 GTCAC ^GT[GC]AC
Tth111I 625 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 968 TCGA T^CGA
XcmI 998 CCAACGACCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 600 GGATCT [GA]^GATC[CT]
XmnI 330 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AlwI 600 GGATC GGATC(4/5)
905 GGATC
AspHI 346 GAGCTC G[AT]GC[AT]^C
883 GTGCTC
AvaI 82 CCCGGG C^[CT]CG[GA]G
94 CCCGGG
BanII 91 GGGCCC G[GA]GC[CT]^C
346 GAGCTC
BbsI 568 GTCTTC GAAGAC(2/6)
631 GTCTTC
BbvCI 139 CCTCAGC CCTCAGC(-5/-2)
525 GCTGAGG
BlpI 531 GCTGAGC GC^T[ACGT]AGC
618 GCTGAGC
Bpu1102I 531 GCTGAGC GC^T[ACGT]AGC
618 GCTGAGC
BpuAI 568 GTCTTC GAAGAC(2/6)
631 GTCTTC
BsiHKAI 346 GAGCTC G[AT]GC[AT]^C
883 GTGCTC
BsmI 226 GAATGC GAATGC(1/-1)
369 GAATGC
BsoBI 82 CCCGGG C^[CT]CG[GA]G
94 CCCGGG
BsrI 88 ACTGG ACTGG(1/-1)
480 CCAGT
CelII 531 GCTGAGC GC^T[ACGT]AGC
618 GCTGAGC
CfoI 99 GCGC GCG^C
1056 GCGC
DpnII 601 GATC ^GATC
906 GATC
DsaI 651 CCATGG C^C[GA][CT]GG
1136 CCGTGG
EcoT14I 54 CCAAGG C^C[AT][AT]GG
651 CCATGG
HhaI 99 GCGC GCG^C
1056 GCGC
HinP1I 99 GCGC G^CGC
1056 GCGC
HphI 7 TCACC GGTGA(8/7)
732 TCACC
MaeIII 6 GTCAC ^GT[ACGT]AC
752 GTAAC
MboI 601 GATC ^GATC
906 GATC
MspA1I 523 CAGCTG C[AC]G^C[GT]G
1099 CCGCTG
NdeII 601 GATC ^GATC
906 GATC
PleI 845 GACTC GAGTC(4/5)
358 GAGTC
Sau3AI 601 GATC ^GATC
906 GATC
SmaI 82 CCCGGG CCC^GGG
94 CCCGGG
StyI 54 CCAAGG C^C[AT][AT]GG
651 CCATGG
TfiI 410 GAATC G^A[AT]TC
554 GAATC
XmaCI 82 CCCGGG C^CCGGG
94 CCCGGG
XmaI 82 CCCGGG C^CCGGG
94 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AluI AvaII BbvI BcnI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmFI Bsp1286I BspMI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DraII EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
ItaI MboII MnlI MseI MspI
MvaI MwoI NciI NlaIII NlaIV
PpuMI Sau96I ScrFI SfaNI Tru9I
TseI TspRI