Restriction map for
pF1KB3506
##################################
# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspHI AspI AviII
AvrII BamHI BbrPI BbvCI BcgI
BciVI BfaI BfrI BglII BlnI
BsaAI BsaI BseAI BsgI BsiHKAI
BsiWI BsmAI BsmBI BsmI BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FseI FspI HgaI HincII HindII
HindIII HinfI HpaI KpnI MaeI
MfeI MflI MluI MroI MslI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TfiI Tsp509I Tth111I
XbaI XhoI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AlwNI 409 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 21 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 402 CTCGGG C^[CT]CG[GA]G
BanII 659 GAGCCC G[GA]GC[CT]^C
BbsI 73 GTCTTC GAAGAC(2/6)
BclI 602 TGATCA T^GATCA
BglI 181 GCCACGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 73 GTCTTC GAAGAC(2/6)
BsaBI 264 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 473 ACCGGA [AT]^CCGG[AT]
BsiEI 471 CGACCG CG[GA][CT]^CG
BsmFI 433 GGGAC GGGAC(10/14)
BsoBI 402 CTCGGG C^[CT]CG[GA]G
Bsp1407I 287 TGTACA T^GTACA
BsrDI 377 GCAATG GCAATG(2/0)
BsrGI 287 TGTACA T^GTACA
DsaI 596 CCGCGG C^C[GA][CT]GG
EarI 25 CTCTTC CTCTTC(1/4)
FbaI 602 TGATCA T^GATCA
Ksp632I 25 CTCTTC CTCTTC(1/4)
KspI 596 CCGCGG CCGC^GG
MaeII 146 ACGT A^CGT
MamI 264 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 572 TTAA T^TAA
NlaIII 285 CATG CATG^
PpuMI 433 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 129 GACGGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 596 CCGCGG CCGC^GG
SapI 24 GCTCTTC GCTCTTC(1/4)
SfcI 524 CTGTAG C^T[GA][CT]AG
SspBI 287 TGTACA T^GTACA
TaqI 422 TCGA T^CGA
Tru9I 572 TTAA T^TAA
TthHB8I 422 TCGA T^CGA
XcmI 737 CCAGCGGCCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 21 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 597 CGCG CG^CG
721 CGCG
AcyI 214 GGCGCC G[GA]^CG[CT]C
479 GGCGCC
AfaI 288 GTAC GT^AC
670 GTAC
BalI 2 TGGCCA TGG^CCA
56 TGGCCA
BanI 214 GGCGCC G^G[CT][GA]CC
479 GGCGCC
BbeI 214 GGCGCC GGCGC^C
479 GGCGCC
BcnI 111 CCCGG CC^[GC]GG
228 CCCGG
BmyI 278 GTGCCC G[AGT]GC[ACT]^C
659 GAGCCC
BsaHI 214 GGCGCC G[GA]^CG[CT]C
479 GGCGCC
BseRI 348 CTCCTC GAGGAG(10/8)
751 GAGGAG
Bsp1286I 278 GTGCCC G[AGT]GC[ACT]^C
659 GAGCCC
BstUI 597 CGCG CG^CG
721 CGCG
DraII 410 AGGGCCT [GA]G^G[ACGT]CC[CT]
433 GGGACCC
EcoO109I 410 AGGGCCT [GA]G^G[ACGT]CC[CT]
433 GGGACCC
EcoT14I 489 CCTTGG C^C[AT][AT]GG
531 CCTTGG
HaeII 214 GGCGCC [GA]GCGC^[CT]
479 GGCGCC
Hin1I 214 GGCGCC G[GA]^CG[CT]C
479 GGCGCC
HphI 600 GGTGA GGTGA(8/7)
344 TCACC
KasI 214 GGCGCC G^GCGCC
479 GGCGCC
MluNI 2 TGGCCA TGG^CCA
56 TGGCCA
MscI 2 TGGCCA TGG^CCA
56 TGGCCA
MvnI 597 CGCG CG^CG
721 CGCG
NarI 214 GGCGCC GG^CGCC
479 GGCGCC
NciI 111 CCCGG CC^[GC]GG
228 CCCGG
PflMI 300 CCAGTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
408 CCAGGGCCTGG
PvuII 255 CAGCTG CAG^CTG
442 CAGCTG
RsaI 288 GTAC GT^AC
670 GTAC
SfoI 214 GGCGCC GGC^GCC
479 GGCGCC
StyI 489 CCTTGG C^C[AT][AT]GG
531 CCTTGG
Van91I 300 CCAGTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
408 CCAGGGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AluI AvaII BbvI BlpI
BpmI Bpu1102I BsaJI BsiYI BslI
BsrI BssKI BstF5I BstNI Cac8I
CelII CfoI Cfr13I DdeI DpnII
EaeI EcoRII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII ItaI
MaeIII MboI MboII MnlI MspA1I
MspI MvaI MwoI NdeII NlaIV
Sau3AI Sau96I ScrFI SfaNI TseI
Tsp45I TspRI