Restriction map for
pF1KB3501
##################################
# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BalI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BglI BlnI
BpmI BpuAI BsaBI BsaI BseAI
BseRI BsgI BsiEI BsiWI BsmAI
BsmBI BspDI BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRV EcoT14I FbaI FseI
FspI HpaI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I StuI
StyI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccII 168 CGCG CG^CG
AciI 169 GCGG CCGC(-3/-1)
AcyI 144 GGCGCC G[GA]^CG[CT]C
AspHI 897 GAGCAC G[AT]GC[AT]^C
AspI 1122 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 678 GGACC G^G[AT]CC
BanI 144 GGCGCC G^G[CT][GA]CC
BanII 1108 GGGCTC G[GA]GC[CT]^C
BbeI 144 GGCGCC GGCGC^C
BfaI 202 CTAG C^TAG
BglII 996 AGATCT A^GATCT
BsaAI 88 TACGTG [CT]AC^GT[GA]
BsaHI 144 GGCGCC G[GA]^CG[CT]C
BsiHKAI 897 GAGCAC G[AT]GC[AT]^C
BsmI 1094 GAATGC GAATGC(1/-1)
BstF5I 639 CATCC GGATG(2/0)
BstUI 168 CGCG CG^CG
DraI 702 TTTAAA TTT^AAA
EarI 940 GAAGAG CTCTTC(1/4)
EcoNI 96 CCTGAAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 228 GAATTC G^AATTC
EcoT22I 1096 ATGCAT ATGCA^T
FauI 169 GCGGG CCCGC(4/6)
FokI 639 CATCC GGATG(9/13)
HaeII 144 GGCGCC [GA]GCGC^[CT]
HgaI 56 GCGTC GACGC(5/10)
Hin1I 144 GGCGCC G[GA]^CG[CT]C
HindIII 709 AAGCTT A^AGCTT
KasI 144 GGCGCC G^GCGCC
Ksp632I 940 GAAGAG CTCTTC(1/4)
MaeI 202 CTAG C^TAG
MaeII 89 ACGT A^CGT
MvnI 168 CGCG CG^CG
NarI 144 GGCGCC GG^CGCC
NsiI 1096 ATGCAT ATGCA^T
PflFI 1122 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SfoI 144 GGCGCC GGC^GCC
TaqI 323 TCGA T^CGA
TfiI 173 GATTC G^A[AT]TC
Tth111I 1122 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 323 TCGA T^CGA
XbaI 201 TCTAGA T^CTAGA
XcmI 591 CCAAAGAGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AvaI 20 CCCGGG C^[CT]CG[GA]G
138 CCCGGG
BamHI 506 GGATCC G^GATCC
731 GGATCC
BbvI 1065 GCTGC GCAGC(8/12)
1083 GCTGC
BlpI 51 GCTCAGC GC^T[ACGT]AGC
894 GCTGAGC
Bpu1102I 51 GCTCAGC GC^T[ACGT]AGC
894 GCTGAGC
BsaWI 176 TCCGGT [AT]^CCGG[AT]
431 TCCGGT
BsmFI 93 GTCCC GGGAC(10/14)
1056 GTCCC
BsoBI 20 CCCGGG C^[CT]CG[GA]G
138 CCCGGG
Bsp1407I 155 TGTACA T^GTACA
914 TGTACA
BspLU11I 158 ACATGT A^CATGT
849 ACATGT
BsrGI 155 TGTACA T^GTACA
914 TGTACA
Cac8I 133 GCCAGC GC[ACGT]^[ACGT]GC
1068 GCCTGC
CelII 51 GCTCAGC GC^T[ACGT]AGC
894 GCTGAGC
CfoI 145 GCGC GCG^C
190 GCGC
DraIII 1041 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
1130 CACACTGTG
EaeI 829 TGGCCG [CT]^GGCC[GA]
964 TGGCCG
Fnu4HI 1065 GCTGC GC^[ACGT]GC
1083 GCTGC
HhaI 145 GCGC GCG^C
190 GCGC
HinP1I 145 GCGC G^CGC
190 GCGC
HincII 781 GTTGAC GT[CT]^[GA]AC
853 GTCAAC
HindII 781 GTTGAC GT[CT]^[GA]AC
853 GTCAAC
ItaI 1065 GCTGC GC^[ACGT]GC
1083 GCTGC
MspA1I 1063 CAGCTG C[AC]G^C[GT]G
1081 CAGCTG
PflMI 722 CCATTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1040 CCACCAAGTGG
PleI 1122 GACTC GAGTC(4/5)
1054 GAGTC
PvuII 1063 CAGCTG CAG^CTG
1081 CAGCTG
SmaI 20 CCCGGG CCC^GGG
138 CCCGGG
SspBI 155 TGTACA T^GTACA
914 TGTACA
SspI 478 AATATT AAT^ATT
650 AATATT
TseI 1065 GCTGC G^C[AT]GC
1083 GCTGC
Van91I 722 CCATTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1040 CCACCAAGTGG
XmaCI 20 CCCGGG C^CCGGG
138 CCCGGG
XmaI 20 CCCGGG C^CCGGG
138 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AcsI AfaI AflIII AluI AlwI
AlwNI ApoI Asp700I BcnI BmyI
BsaJI BsiYI BslI Bsp1286I BsrI
BssKI BstNI BstXI BstYI Cfr13I
DdeI DpnII EcoRII HaeIII HapII
HinfI HpaII HphI MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI RsaI Sau3AI
Sau96I ScrFI SfaNI Tru9I Tsp45I
Tsp509I TspRI XhoII XmnI