Restriction map for
pF1KB3474
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# NO CUT ENZYMES ( 154 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MaeII MamI MfeI MluI MroI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tth111I TthHB8I
Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcyI 402 GGCGTC G[GA]^CG[CT]C
BalI 446 TGGCCA TGG^CCA
BamHI 433 GGATCC G^GATCC
BbsI 364 GAAGAC GAAGAC(2/6)
BbvI 373 GCAGC GCAGC(8/12)
BglI 34 GCCGAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 349 CTCCAG CTGGAG(16/14)
BpuAI 364 GAAGAC GAAGAC(2/6)
BsaHI 402 GGCGTC G[GA]^CG[CT]C
BsaI 414 GAGACC GGTCTC(1/5)
BsgI 371 GTGCAG GTGCAG(16/14)
BsmFI 166 GGGAC GGGAC(10/14)
BsmI 222 GAATGC GAATGC(1/-1)
BsrI 44 CCAGT ACTGG(1/-1)
BstYI 433 GGATCC [GA]^GATC[CT]
Cac8I 13 GCAGGC GC[ACGT]^[ACGT]GC
DsaI 461 CCATGG C^C[GA][CT]GG
EaeI 446 TGGCCA [CT]^GGCC[GA]
EarI 411 GAAGAG CTCTTC(1/4)
EcoNI 419 CCTCCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 561 GCGGG CCCGC(4/6)
HaeII 527 GGCGCT [GA]GCGC^[CT]
HapII 298 CCGG C^CGG
HgaI 403 GCGTC GACGC(5/10)
Hin1I 402 GGCGTC G[GA]^CG[CT]C
HpaII 298 CCGG C^CGG
Ksp632I 411 GAAGAG CTCTTC(1/4)
MflI 433 GGATCC [GA]^GATC[CT]
MluNI 446 TGGCCA TGG^CCA
MscI 446 TGGCCA TGG^CCA
MseI 28 TTAA T^TAA
MslI 183 CACAGCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 298 CCGG C^CGG
NcoI 461 CCATGG C^CATGG
PpuMI 166 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 245 CAGCTG CAG^CTG
SmaI 297 CCCGGG CCC^GGG
TfiI 73 GAATC G^A[AT]TC
Tru9I 28 TTAA T^TAA
TseI 373 GCAGC G^C[AT]GC
Tsp509I 26 AATT ^AATT
TspRI 369 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 468 CCATGCCAGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 433 GGATCC [GA]^GATC[CT]
XmaCI 297 CCCGGG C^CCGGG
XmaI 297 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AspHI 346 GAGCTC G[AT]GC[AT]^C
579 GAGCTC
AvaI 287 CCCGAG C^[CT]CG[GA]G
297 CCCGGG
BcnI 297 CCCGG CC^[GC]GG
298 CCGGG
BsiHKAI 346 GAGCTC G[AT]GC[AT]^C
579 GAGCTC
BsmAI 414 GAGAC GTCTC(1/5)
500 GAGAC
BsoBI 287 CCCGAG C^[CT]CG[GA]G
297 CCCGGG
BstXI 156 CCAGAACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
437 CCAAGTACCTGG
DpnII 273 GATC ^GATC
434 GATC
DraII 166 GGGACCT [GA]G^G[ACGT]CC[CT]
328 AGGCCCT
EcoO109I 166 GGGACCT [GA]G^G[ACGT]CC[CT]
328 AGGCCCT
EcoT14I 461 CCATGG C^C[AT][AT]GG
490 CCAAGG
Fnu4HI 260 GCCGC GC^[ACGT]GC
373 GCAGC
ItaI 260 GCCGC GC^[ACGT]GC
373 GCAGC
MboI 273 GATC ^GATC
434 GATC
NciI 297 CCCGG CC^[GC]GG
298 CCGGG
NdeII 273 GATC ^GATC
434 GATC
SacI 346 GAGCTC GAGCT^C
579 GAGCTC
Sau3AI 273 GATC ^GATC
434 GATC
StyI 461 CCATGG C^C[AT][AT]GG
490 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanII BmyI BsaJI BseRI BsiYI
BslI Bsp1286I BssKI BstF5I BstNI
CfoI Cfr13I DdeI EcoRII FokI
HaeIII HhaI HinP1I HinfI HphI
MaeIII MboII MnlI MspA1I MvaI
MwoI NlaIII NlaIV PleI RsaI
Sau96I ScrFI SfaNI Tsp45I