Restriction map for
pF1KB3455
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# NO CUT ENZYMES ( 185 enzymes) #
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AatII Acc65I AccI AccII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI AlwI AlwNI AocI
Aor51HI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsgI BsiEI BsiWI BsmAI
BsmBI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstNI BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DpnII DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRII EcoRV EcoT22I FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HphI KasI KpnI KspI MaeIII
MamI MboI MfeI MflI MluI
MluNI MscI MseI MspA1I MunI
MvaI MvnI NaeI NarI NcoI
NdeI NdeII NgoMIV NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI Sau3AI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Tsp45I Tsp509I Van91I
XbaI XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AccIII 464 TCCGGA T^CCGGA
Alw44I 299 GTGCAC G^TGCAC
ApaI 179 GGGCCC GGGCC^C
ApaLI 299 GTGCAC G^TGCAC
AspHI 299 GTGCAC G[AT]GC[AT]^C
AspI 142 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 176 CCCGGG C^[CT]CG[GA]G
AvaII 108 GGACC G^G[AT]CC
BfaI 190 CTAG C^TAG
BsaWI 464 TCCGGA [AT]^CCGG[AT]
BseAI 464 TCCGGA T^CCGGA
BseRI 348 CTCCTC GAGGAG(10/8)
BsiHKAI 299 GTGCAC G[AT]GC[AT]^C
BsmI 273 GAATGC GAATGC(1/-1)
BsoBI 176 CCCGGG C^[CT]CG[GA]G
BspEI 464 TCCGGA T^CCGGA
Cac8I 475 GCCAGC GC[ACGT]^[ACGT]GC
FauI 138 GCGGG CCCGC(4/6)
MaeI 190 CTAG C^TAG
MaeII 102 ACGT A^CGT
MroI 464 TCCGGA T^CCGGA
PflFI 142 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 331 CTGCAG CTGCA^G
SfaNI 172 GCATC GCATC(5/9)
SfcI 331 CTGCAG C^T[GA][CT]AG
SmaI 176 CCCGGG CCC^GGG
SmlI 262 CTCAAG C^T[CT][GA]AG
Tth111I 142 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 176 CCCGGG C^CCGGG
XmaI 176 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
BbvI 288 GCAGC GCAGC(8/12)
383 GCAGC
BcnI 176 CCCGG CC^[GC]GG
177 CCGGG
BssKI 176 CCCGG ^CC[ACGT]GG
177 CCGGG
BstF5I 173 CATCC GGATG(2/0)
467 GGATG
EcoT14I 159 CCAAGG C^C[AT][AT]GG
377 CCAAGG
FokI 173 CATCC GGATG(9/13)
467 GGATG
HaeIII 180 GGCC GG^CC
474 GGCC
HapII 177 CCGG C^CGG
465 CCGG
HpaII 177 CCGG C^CGG
465 CCGG
MslI 18 CATCGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
79 CATCATAATG
MspI 177 CCGG C^CGG
465 CCGG
MwoI 172 GCATCCCGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
435 GCCTGAAGAGC
NciI 176 CCCGG CC^[GC]GG
177 CCGGG
NlaIV 107 GGGACC GG[ACGT]^[ACGT]CC
179 GGGCCC
ScrFI 176 CCCGG CC^[ACGT]GG
177 CCGGG
StyI 159 CCAAGG C^C[AT][AT]GG
377 CCAAGG
TaqI 20 TCGA T^CGA
401 TCGA
TseI 288 GCAGC G^C[AT]GC
383 GCAGC
TspRI 89 CACTG CA[GC]TG[ACGT][ACGT]^
302 CACTG
TthHB8I 20 TCGA T^CGA
401 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AciI AluI BanII BmyI BpmI
BsaJI BsiYI BslI BsmFI Bsp1286I
BsrI Cfr13I DdeI EarI Fnu4HI
HinfI ItaI Ksp632I MboII MnlI
PleI SapI Sau96I