Restriction map for
pF1KB3449
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# NO CUT ENZYMES ( 189 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbvCI BbvI BcgI BciVI BclI
BcnI BfrI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII HapII HgaI
Hin1I HincII HindII HindIII HpaI
HpaII KasI KpnI Ksp632I KspI
MaeII MaeIII MamI MfeI MflI
MluI MluNI MroI MscI MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SphI Sse8387I SspBI StyI
SwaI TaqI TseI Tsp45I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AciI 450 CCGC CCGC(-3/-1)
AcsI 193 GAATTT [GA]^AATT[CT]
ApoI 193 GAATTT [GA]^AATT[CT]
AvaII 292 GGACC G^G[AT]CC
BanI 26 GGCACC G^G[CT][GA]CC
BbsI 3 GTCTTC GAAGAC(2/6)
BglI 518 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 3 GTCTTC GAAGAC(2/6)
BsaJI 158 CCACGG C^C[ACGT][ACGT]GG
BsiYI 450 CCGCTCTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 450 CCGCTCTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 291 GGGAC GGGAC(10/14)
BsrBI 450 CCGCTC CCGCTC(-3/-3)
BssKI 535 CCTGG ^CC[ACGT]GG
BstF5I 459 GGATG GGATG(2/0)
BstNI 535 CCTGG CC^[AT]GG
Cac8I 523 GCTGGC GC[ACGT]^[ACGT]GC
CfoI 327 GCGC GCG^C
DraI 76 TTTAAA TTT^AAA
DsaI 158 CCACGG C^C[GA][CT]GG
EaeI 447 TGGCCG [CT]^GGCC[GA]
EcoRII 535 CCTGG ^CC[AT]GG
EcoRV 82 GATATC GAT^ATC
Fnu4HI 449 GCCGC GC^[ACGT]GC
FokI 459 GGATG GGATG(9/13)
HhaI 327 GCGC GCG^C
HinP1I 327 GCGC G^CGC
HinfI 235 GATTC G^A[ACGT]TC
HphI 143 TCACC GGTGA(8/7)
ItaI 449 GCCGC GC^[ACGT]GC
MslI 144 CACCTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 535 CCTGG CC^[AT]GG
MwoI 518 GCCCAGCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PpuMI 291 GGGACCC [GA]G^G[AT]CC[CT]
ScrFI 535 CCTGG CC^[ACGT]GG
SfaNI 371 GCATC GCATC(5/9)
SpeI 474 ACTAGT A^CTAGT
SspI 578 AATATT AAT^ATT
StuI 482 AGGCCT AGG^CCT
TfiI 235 GATTC G^A[AT]TC
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# TWO CUT ENZYMES ( 19 enzymes) #
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AluI 54 AGCT AG^CT
522 AGCT
BfaI 253 CTAG C^TAG
475 CTAG
BsmAI 463 GAGAC GTCTC(1/5)
471 GAGAC
BsrDI 168 CATTGC GCAATG(2/0)
333 CATTGC
BsrI 288 ACTGG ACTGG(1/-1)
489 CCAGT
BstXI 213 CCAAGTGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
520 CCAGCTGGCTGG
Cfr13I 292 GGACC G^G[ACGT]CC
384 GGGCC
DdeI 272 CTAAG C^T[ACGT]AG
338 CTCAG
DpnII 165 GATC ^GATC
423 GATC
DraII 291 GGGACCC [GA]G^G[ACGT]CC[CT]
383 GGGGCCT
EcoO109I 291 GGGACCC [GA]G^G[ACGT]CC[CT]
383 GGGGCCT
MaeI 253 CTAG C^TAG
475 CTAG
MboI 165 GATC ^GATC
423 GATC
MspA1I 53 CAGCTG C[AC]G^C[GT]G
521 CAGCTG
NdeII 165 GATC ^GATC
423 GATC
PvuII 53 CAGCTG CAG^CTG
521 CAGCTG
Sau3AI 165 GATC ^GATC
423 GATC
Sau96I 292 GGACC G^G[ACGT]CC
384 GGGCC
Tsp509I 21 AATT ^AATT
194 AATT
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# 3 OR MORE CUT ENZYMES ( 10 enzymes) #
#########################################
BmyI Bsp1286I HaeIII MboII MnlI
MseI NlaIII NlaIV Tru9I TspRI