Restriction map for
pF1KB3448
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# NO CUT ENZYMES ( 175 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvaI AvaII
AvrII BalI BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfaI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmAI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoT14I EcoT22I FbaI
FseI HaeII HapII HgaI Hin1I
HincII HindII HpaI HpaII KasI
KpnI KspI MaeI MaeII MamI
MfeI MluI MluNI MroI MscI
MslI MspA1I MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI TaqI Tth111I TthHB8I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AlwI 828 GGATC GGATC(4/5)
Asp700I 660 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 863 GAGCTC G[AT]GC[AT]^C
AviII 226 TGCGCA TGC^GCA
BanII 863 GAGCTC G[GA]GC[CT]^C
BbsI 62 GAAGAC GAAGAC(2/6)
BglII 350 AGATCT A^GATCT
BpmI 695 CTCCAG CTGGAG(16/14)
BpuAI 62 GAAGAC GAAGAC(2/6)
BsaJI 540 CCCTGG C^C[ACGT][ACGT]GG
BseRI 706 GAGGAG GAGGAG(10/8)
BsiHKAI 863 GAGCTC G[AT]GC[AT]^C
BspMI 427 ACCTGC ACCTGC(4/8)
BsrBI 871 GAGCGG CCGCTC(-3/-3)
BstYI 350 AGATCT [GA]^GATC[CT]
EarI 786 GAAGAG CTCTTC(1/4)
EcoRV 175 GATATC GAT^ATC
FspI 226 TGCGCA TGC^GCA
HindIII 852 AAGCTT A^AGCTT
Ksp632I 786 GAAGAG CTCTTC(1/4)
MflI 350 AGATCT [GA]^GATC[CT]
NspI 620 ACATGC [GA]CATG^[CT]
PflMI 250 CCAAAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 287 CTGCAG CTGCA^G
SacI 863 GAGCTC GAGCT^C
SfcI 287 CTGCAG C^T[GA][CT]AG
TspRI 462 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 250 CCAAAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 350 AGATCT [GA]^GATC[CT]
XmnI 660 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AciI 318 CCGC CCGC(-3/-1)
873 GCGG
AcsI 55 AAATTT [GA]^AATT[CT]
565 GAATTT
ApoI 55 AAATTT [GA]^AATT[CT]
565 GAATTT
BanI 418 GGTGCC G^G[CT][GA]CC
481 GGTGCC
BmyI 482 GTGCCC G[AGT]GC[ACT]^C
863 GAGCTC
BsmFI 800 GTCCC GGGAC(10/14)
810 GTCCC
Bsp1286I 482 GTGCCC G[AGT]GC[ACT]^C
863 GAGCTC
BssKI 415 CCAGG ^CC[ACGT]GG
541 CCTGG
BstAPI 229 GCAGCGTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
442 GCAGGCCTTGC
BstNI 415 CCAGG CC^[AT]GG
541 CCTGG
CfoI 227 GCGC GCG^C
624 GCGC
Cfr13I 315 GGCCC G^G[ACGT]CC
600 GGGCC
DpnII 351 GATC ^GATC
829 GATC
EcoRII 415 CCAGG ^CC[AT]GG
541 CCTGG
FauI 317 CCCGC CCCGC(4/6)
873 GCGGG
HhaI 227 GCGC GCG^C
624 GCGC
HinP1I 227 GCGC G^CGC
624 GCGC
MboI 351 GATC ^GATC
829 GATC
MvaI 415 CCAGG CC^[AT]GG
541 CCTGG
NdeII 351 GATC ^GATC
829 GATC
Sau3AI 351 GATC ^GATC
829 GATC
Sau96I 315 GGCCC G^G[ACGT]CC
600 GGGCC
ScrFI 415 CCAGG CC^[ACGT]GG
541 CCTGG
SfaNI 439 GATGC GCATC(5/9)
649 GATGC
StuI 29 AGGCCT AGG^CCT
444 AGGCCT
Tsp45I 163 GTGAC ^GT[GC]AC
373 GTGAC
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# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AfaI AluI BbvI BsiYI BslI
BsrI BstF5I Cac8I DdeI Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeIII MboII MnlI MseI MwoI
NlaIII NlaIV RsaI TfiI Tru9I
TseI Tsp509I