Restriction map for
pF1KB3446
##################################
# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflIII AgeI AhdI Alw44I
AlwNI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BamHI BbeI BbrPI BbvCI
BcgI BciVI BclI BfaI BglI
BglII BlnI BsaAI BsaBI BsaHI
BsaI BsgI BsiEI BsiWI BsmAI
BsmBI BsmFI BsmI BspDI BspHI
BspLU11I BspMI BsrFI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Cfr10I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HgaI Hin1I KasI KpnI
KspI MaeI MaeII MamI MluI
MspA1I MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
StuI SwaI TaqI Tth111I TthHB8I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AccIII 4 TCCGGA T^CCGGA
AcsI 676 GAATTT [GA]^AATT[CT]
AflII 46 CTTAAG C^TTAAG
AocI 727 CCTGAGG CC^T[ACGT]AGG
Aor51HI 802 AGCGCT AGC^GCT
ApoI 676 GAATTT [GA]^AATT[CT]
AspHI 500 GAGCTC G[AT]GC[AT]^C
AvaI 536 CTCGGG C^[CT]CG[GA]G
AvaII 158 GGACC G^G[AT]CC
AviII 548 TGCGCA TGC^GCA
BalI 246 TGGCCA TGG^CCA
BcnI 348 CCGGG CC^[GC]GG
BfrI 46 CTTAAG C^TTAAG
BlpI 805 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 805 GCTCAGC GC^T[ACGT]AGC
BsaWI 4 TCCGGA [AT]^CCGG[AT]
BseAI 4 TCCGGA T^CCGGA
BsiHKAI 500 GAGCTC G[AT]GC[AT]^C
BsoBI 536 CTCGGG C^[CT]CG[GA]G
Bsp1407I 753 TGTACA T^GTACA
BspEI 4 TCCGGA T^CCGGA
BsrBI 701 CCGCTC CCGCTC(-3/-3)
BsrGI 753 TGTACA T^GTACA
BssSI 185 CTCGTG CACGAG(-5/-1)
BstXI 69 CCACTTAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 727 CCTGAGG CC^T[ACGT]AGG
CelII 805 GCTCAGC GC^T[ACGT]AGC
Cfr13I 158 GGACC G^G[ACGT]CC
DraII 157 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 505 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 802 AGCGCT AGC^GCT
Eco81I 727 CCTGAGG CC^T[ACGT]AGG
EcoO109I 157 AGGACCT [GA]G^G[ACGT]CC[CT]
FauI 700 CCCGC CCCGC(4/6)
FspI 548 TGCGCA TGC^GCA
HaeII 802 AGCGCT [GA]GCGC^[CT]
HincII 418 GTTAAC GT[CT]^[GA]AC
HindII 418 GTTAAC GT[CT]^[GA]AC
HindIII 169 AAGCTT A^AGCTT
HinfI 451 GATTC G^A[ACGT]TC
HpaI 418 GTTAAC GTT^AAC
HphI 685 GGTGA GGTGA(8/7)
MluNI 246 TGGCCA TGG^CCA
MroI 4 TCCGGA T^CCGGA
MscI 246 TGGCCA TGG^CCA
MslI 70 CACTTAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 348 CCGGG CC^[GC]GG
NcoI 580 CCATGG C^CATGG
NspI 551 GCATGC [GA]CATG^[CT]
PpuMI 157 AGGACCT [GA]G^G[AT]CC[CT]
PstI 830 CTGCAG CTGCA^G
SacI 500 GAGCTC GAGCT^C
SapI 637 GAAGAGC GCTCTTC(1/4)
Sau96I 158 GGACC G^G[ACGT]CC
SphI 551 GCATGC GCATG^C
SspBI 753 TGTACA T^GTACA
TfiI 451 GATTC G^A[AT]TC
Tsp45I 664 GTGAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AciI 303 CCGC CCGC(-3/-1)
701 CCGC
AlwI 333 GATCC GGATC(4/5)
616 GATCC
BanII 8 GAGCCC G[GA]GC[CT]^C
500 GAGCTC
BbsI 793 GAAGAC GAAGAC(2/6)
820 GAAGAC
BpuAI 793 GAAGAC GAAGAC(2/6)
820 GAAGAC
BseRI 455 CTCCTC GAGGAG(10/8)
34 GAGGAG
BsrDI 432 CATTGC GCAATG(2/0)
474 CATTGC
BstAPI 181 GCAGCTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
190 GCAATTGTTGC
BstYI 332 AGATCC [GA]^GATC[CT]
615 AGATCC
EaeI 246 TGGCCA [CT]^GGCC[GA]
300 TGGCCG
EarI 622 GAAGAG CTCTTC(1/4)
637 GAAGAG
EcoT14I 580 CCATGG C^C[AT][AT]GG
668 CCAAGG
HapII 5 CCGG C^CGG
348 CCGG
HpaII 5 CCGG C^CGG
348 CCGG
Ksp632I 622 GAAGAG CTCTTC(1/4)
637 GAAGAG
MfeI 191 CAATTG C^AATTG
293 CAATTG
MflI 332 AGATCC [GA]^GATC[CT]
615 AGATCC
MseI 47 TTAA T^TAA
419 TTAA
MspI 5 CCGG C^CGG
348 CCGG
MunI 191 CAATTG C^AATTG
293 CAATTG
SfcI 609 CTACAG C^T[GA][CT]AG
830 CTGCAG
SmlI 46 CTTAAG C^T[CT][GA]AG
151 CTCAAG
StyI 580 CCATGG C^C[AT][AT]GG
668 CCAAGG
Tru9I 47 TTAA T^TAA
419 TTAA
XhoII 332 AGATCC [GA]^GATC[CT]
615 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AfaI AluI BanI BbvI BmyI
BpmI BsaJI BsiYI BslI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI DdeI DpnII DsaI EcoRII
Fnu4HI FokI HaeIII HhaI HinP1I
ItaI MaeIII MboI MboII MnlI
MvaI MwoI NdeII NlaIII NlaIV
PflMI RsaI Sau3AI ScrFI SfaNI
TseI Tsp509I TspRI Van91I