Restriction map for
pF1KB3427
##################################
# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII AccII AcyI AflII AflIII
AgeI AhdI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I AspEI
AspI AvaI AviII AvrII BamHI
BanII BbeI BbsI BcgI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaBI BsaHI BsaI
BsiEI BsiWI BsmBI BsmFI BsoBI
Bsp1407I BspDI BspHI BspLU11I BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HgaI Hin1I HincII HindII
HpaI KasI KspI MamI MfeI
MluI MunI MvnI NaeI NarI
NgoMIV NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmeI PpuMI PshBI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 598 GGTACC G^GTACC
AccI 452 GTCTAC GT^[AC][GT]AC
AccIII 107 TCCGGA T^CCGGA
AcsI 103 AAATTC [GA]^AATT[CT]
AlwNI 962 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 103 AAATTC [GA]^AATT[CT]
Asp718I 598 GGTACC G^GTACC
AvaII 948 GGACC G^G[AT]CC
BanI 598 GGTACC G^G[CT][GA]CC
BbrPI 271 CACGTG CAC^GTG
BbvCI 232 GCTGAGG CCTCAGC(-5/-2)
BciVI 1218 GTATCC GTATCC(6/5)
BfaI 767 CTAG C^TAG
BsaAI 271 CACGTG [CT]AC^GT[GA]
BsaWI 107 TCCGGA [AT]^CCGG[AT]
BseAI 107 TCCGGA T^CCGGA
BseRI 1210 GAGGAG GAGGAG(10/8)
BsmAI 347 GAGAC GTCTC(1/5)
BsmI 1115 GCATTC GAATGC(1/-1)
BspEI 107 TCCGGA T^CCGGA
BsrBI 39 CCGCTC CCGCTC(-3/-3)
BstAPI 1122 GCAAGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 993 GGATG GGATG(2/0)
Cfr13I 948 GGACC G^G[ACGT]CC
DsaI 1163 CCATGG C^C[GA][CT]GG
FokI 993 GGATG GGATG(9/13)
HindIII 400 AAGCTT A^AGCTT
KpnI 598 GGTACC GGTAC^C
MaeI 767 CTAG C^TAG
MaeIII 676 GTGAC ^GT[ACGT]AC
MroI 107 TCCGGA T^CCGGA
NcoI 1163 CCATGG C^CATGG
NdeI 1235 CATATG CA^TATG
NheI 766 GCTAGC G^CTAGC
PmaCI 271 CACGTG CAC^GTG
PmlI 271 CACGTG CAC^GTG
PshAI 936 GACCCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Sau96I 948 GGACC G^G[ACGT]CC
ScaI 750 AGTACT AGT^ACT
TaqI 215 TCGA T^CGA
Tsp45I 676 GTGAC ^GT[GC]AC
TthHB8I 215 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Alw44I 274 GTGCAC G^TGCAC
784 GTGCAC
ApaLI 274 GTGCAC G^TGCAC
784 GTGCAC
AspHI 274 GTGCAC G[AT]GC[AT]^C
784 GTGCAC
BcnI 69 CCCGG CC^[GC]GG
585 CCGGG
BmyI 274 GTGCAC G[AGT]GC[ACT]^C
784 GTGCAC
BsgI 485 CTGCAC GTGCAG(16/14)
1192 GTGCAG
BsiHKAI 274 GTGCAC G[AT]GC[AT]^C
784 GTGCAC
Bsp1286I 274 GTGCAC G[AGT]GC[ACT]^C
784 GTGCAC
BspMI 386 ACCTGC ACCTGC(4/8)
575 ACCTGC
CfoI 579 GCGC GCG^C
1113 GCGC
EarI 238 GAAGAG CTCTTC(1/4)
1207 GAAGAG
EcoT14I 321 CCTTGG C^C[AT][AT]GG
1163 CCATGG
HhaI 579 GCGC GCG^C
1113 GCGC
HinP1I 579 GCGC G^CGC
1113 GCGC
Ksp632I 238 GAAGAG CTCTTC(1/4)
1207 GAAGAG
MboII 238 GAAGA GAAGA(8/7)
1207 GAAGA
MseI 695 TTAA T^TAA
1228 TTAA
NciI 69 CCCGG CC^[GC]GG
585 CCGGG
SmlI 823 CTCAAG C^T[CT][GA]AG
864 CTCAAG
StyI 321 CCTTGG C^C[AT][AT]GG
1163 CCATGG
Tru9I 695 TTAA T^TAA
1228 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AclI AfaI AluI AlwI
BalI BbvI BpmI BsaJI BsiYI
BslI BsrI BssKI BstNI BstYI
Cac8I DdeI DpnII EaeI EcoRII
FauI Fnu4HI HaeIII HapII HinfI
HpaII HphI ItaI MaeII MboI
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV Psp1406I PstI PvuII
RsaI Sau3AI ScrFI SfaNI SfcI
TfiI TseI Tsp509I TspRI XhoII