Restriction map for
pF1KB3421
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# NO CUT ENZYMES ( 171 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII BamHI BanI
BanII BbeI BbsI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlpI BmyI Bpu1102I BpuAI
BsaBI BsaHI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI Hin1I HindIII KasI KpnI
KspI MamI MfeI MflI MluI
MroI MunI MvnI NarI NciI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tsp45I Tsp509I Tth111I TthHB8I Van91I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AflIII 521 ACGTGT A^C[GA][CT]GT
AlwI 471 GATCC GGATC(4/5)
AvaI 77 CTCGGG C^[CT]CG[GA]G
AvaII 576 GGACC G^G[AT]CC
AvrII 327 CCTAGG C^CTAGG
BalI 272 TGGCCA TGG^CCA
BlnI 327 CCTAGG C^CTAGG
BpmI 104 CTGGAG CTGGAG(16/14)
BsaI 482 GAGACC GGTCTC(1/5)
BsiYI 17 CCAAGAAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 17 CCAAGAAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI 77 CTCGGG C^[CT]CG[GA]G
BsrDI 294 CATTGC GCAATG(2/0)
BsrFI 188 GCCGGC [GA]^CCGG[CT]
BstF5I 459 GGATG GGATG(2/0)
CfoI 516 GCGC GCG^C
Cfr10I 188 GCCGGC [GA]^CCGG[CT]
EaeI 272 TGGCCA [CT]^GGCC[GA]
EcoT14I 327 CCTAGG C^C[AT][AT]GG
FokI 459 GGATG GGATG(9/13)
HapII 189 CCGG C^CGG
HhaI 516 GCGC GCG^C
HinP1I 516 GCGC G^CGC
HpaI 490 GTTAAC GTT^AAC
HpaII 189 CCGG C^CGG
MluNI 272 TGGCCA TGG^CCA
MscI 272 TGGCCA TGG^CCA
MseI 491 TTAA T^TAA
MspA1I 477 CAGCGG C[AC]G^C[GT]G
MspI 189 CCGG C^CGG
NaeI 188 GCCGGC GCC^GGC
NgoMIV 188 GCCGGC G^CCGGC
PleI 307 GAGTC GAGTC(4/5)
PpuMI 575 GGGACCC [GA]G^G[AT]CC[CT]
SfcI 212 CTGTAG C^T[GA][CT]AG
StyI 327 CCTAGG C^C[AT][AT]GG
TfiI 195 GATTC G^A[AT]TC
Tru9I 491 TTAA T^TAA
XcmI 446 CCATGAAGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
BbrPI 520 CACGTG CAC^GTG
619 CACGTG
BfaI 278 CTAG C^TAG
328 CTAG
BsaAI 520 CACGTG [CT]AC^GT[GA]
619 CACGTG
BsmAI 482 GAGAC GTCTC(1/5)
309 GTCTC
BsmFI 575 GGGAC GGGAC(10/14)
718 GTCCC
BsrI 583 ACTGG ACTGG(1/-1)
715 CCAGT
DpnII 471 GATC ^GATC
561 GATC
DraII 349 AGGGCCT [GA]G^G[ACGT]CC[CT]
575 GGGACCC
EarI 21 GAAGAG CTCTTC(1/4)
222 GAAGAG
EcoO109I 349 AGGGCCT [GA]G^G[ACGT]CC[CT]
575 GGGACCC
HincII 490 GTTAAC GT[CT]^[GA]AC
502 GTTGAC
HindII 490 GTTAAC GT[CT]^[GA]AC
502 GTTGAC
HinfI 195 GATTC G^A[ACGT]TC
307 GAGTC
HphI 178 GGTGA GGTGA(8/7)
555 GGTGA
Ksp632I 21 GAAGAG CTCTTC(1/4)
222 GAAGAG
MaeI 278 CTAG C^TAG
328 CTAG
MaeIII 121 GTTAC ^GT[ACGT]AC
317 GTTAC
MboI 471 GATC ^GATC
561 GATC
MslI 507 CACTGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
549 CATCAAGGTG
NdeII 471 GATC ^GATC
561 GATC
PmaCI 520 CACGTG CAC^GTG
619 CACGTG
PmlI 520 CACGTG CAC^GTG
619 CACGTG
Sau3AI 471 GATC ^GATC
561 GATC
SfaNI 44 GCATC GCATC(5/9)
548 GCATC
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# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AciI AluI BbvI BsaJI BssKI
BstNI Cac8I Cfr13I DdeI EcoRII
Fnu4HI HaeIII ItaI MaeII MboII
MnlI MvaI MwoI NlaIII NlaIV
Sau96I ScrFI TseI TspRI