Restriction map for
pF1KB3413
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaI BseAI BseRI
BsgI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KasI KpnI MaeI MaeII MamI
MfeI MluI MroI MseI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tru9I Tth111I
XbaI XcmI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AlwNI 619 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 338 GGGCCC GGGCC^C
AspHI 487 GAGCTC G[AT]GC[AT]^C
BanI 473 GGCACC G^G[CT][GA]CC
BsaWI 110 ACCGGA [AT]^CCGG[AT]
BsiEI 108 CGACCG CG[GA][CT]^CG
BsiHKAI 487 GAGCTC G[AT]GC[AT]^C
BssSI 96 CTCGTG CACGAG(-5/-1)
DdeI 492 CTTAG C^T[ACGT]AG
DraIII 585 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 77 CCGCGG C^C[GA][CT]GG
EcoT14I 938 CCAAGG C^C[AT][AT]GG
HaeII 620 AGCGCC [GA]GCGC^[CT]
HincII 916 GTCAAC GT[CT]^[GA]AC
HindII 916 GTCAAC GT[CT]^[GA]AC
HphI 139 GGTGA GGTGA(8/7)
KspI 77 CCGCGG CCGC^GG
MslI 310 CATGAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PaeR7I 301 CTCGAG C^TCGAG
PpuMI 82 GGGACCC [GA]G^G[AT]CC[CT]
PstI 963 CTGCAG CTGCA^G
SacI 487 GAGCTC GAGCT^C
SacII 77 CCGCGG CCGC^GG
SfcI 963 CTGCAG C^T[GA][CT]AG
SphI 697 GCATGC GCATG^C
StuI 1011 AGGCCT AGG^CCT
StyI 938 CCAAGG C^C[AT][AT]GG
TaqI 302 TCGA T^CGA
Tsp45I 140 GTGAC ^GT[GC]AC
Tsp509I 220 AATT ^AATT
TthHB8I 302 TCGA T^CGA
XhoI 301 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcyI 627 GGCGTC G[GA]^CG[CT]C
662 GGCGTC
AvaI 301 CTCGAG C^[CT]CG[GA]G
609 CCCGAG
BamHI 843 GGATCC G^GATCC
855 GGATCC
BsaHI 627 GGCGTC G[GA]^CG[CT]C
662 GGCGTC
BsoBI 301 CTCGAG C^[CT]CG[GA]G
609 CCCGAG
BsrDI 771 CATTGC GCAATG(2/0)
680 GCAATG
DraII 82 GGGACCC [GA]G^G[ACGT]CC[CT]
338 GGGCCCC
EarI 406 GAAGAG CTCTTC(1/4)
538 GAAGAG
EcoO109I 82 GGGACCC [GA]G^G[ACGT]CC[CT]
338 GGGCCCC
Hin1I 627 GGCGTC G[GA]^CG[CT]C
662 GGCGTC
Ksp632I 406 GAAGAG CTCTTC(1/4)
538 GAAGAG
MaeIII 140 GTGAC ^GT[ACGT]AC
292 GTAAC
NspI 462 GCATGT [GA]CATG^[CT]
697 GCATGC
PflMI 584 CCACTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
618 CCAGCGCCTGG
PleI 7 GACTC GAGTC(4/5)
46 GACTC
PvuII 144 CAGCTG CAG^CTG
784 CAGCTG
TfiI 116 GAATC G^A[AT]TC
580 GAATC
Van91I 584 CCACTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
618 CCAGCGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaII BalI BanII BbvI BcnI
BmyI BsaJI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DpnII EaeI EcoRII FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII ItaI MboI
MboII MflI MluNI MnlI MscI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
TseI TspRI XhoII