Restriction map for
pF1KB3403
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# NO CUT ENZYMES ( 186 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AflIII AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AvaI AvaII AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaJI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstF5I BstPI
BstUI BstXI BstZ17I Bsu36I CelII
Cfr10I Cfr13I ClaI CpoI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoT14I
FauI FbaI FokI FseI HaeII
HapII HgaI Hin1I HincII HindII
HpaI HpaII KasI KpnI KspI
MaeII MaeIII MamI MfeI MluI
MroI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI Sau96I SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspBI SspI StuI StyI
SwaI TaqI Tsp45I Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Asp700I 676 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 3 GAGCTC G[AT]GC[AT]^C
AviII 438 TGCGCA TGC^GCA
BalI 347 TGGCCA TGG^CCA
BanII 3 GAGCTC G[GA]GC[CT]^C
BbsI 668 GAAGAC GAAGAC(2/6)
BfaI 634 CTAG C^TAG
BglII 298 AGATCT A^GATCT
BmyI 3 GAGCTC G[AGT]GC[ACT]^C
BpuAI 668 GAAGAC GAAGAC(2/6)
BsiHKAI 3 GAGCTC G[AT]GC[AT]^C
Bsp1286I 3 GAGCTC G[AGT]GC[ACT]^C
BsrI 16 ACTGG ACTGG(1/-1)
BssKI 506 CCAGG ^CC[ACGT]GG
BstNI 506 CCAGG CC^[AT]GG
BstYI 298 AGATCT [GA]^GATC[CT]
CfoI 439 GCGC GCG^C
EaeI 347 TGGCCA [CT]^GGCC[GA]
EarI 56 GAAGAG CTCTTC(1/4)
EcoRII 506 CCAGG ^CC[AT]GG
EcoRV 565 GATATC GAT^ATC
EcoT22I 362 ATGCAT ATGCA^T
FspI 438 TGCGCA TGC^GCA
HaeIII 348 GGCC GG^CC
HhaI 439 GCGC GCG^C
HinP1I 439 GCGC G^CGC
HphI 652 GGTGA GGTGA(8/7)
Ksp632I 56 GAAGAG CTCTTC(1/4)
MaeI 634 CTAG C^TAG
MflI 298 AGATCT [GA]^GATC[CT]
MluNI 347 TGGCCA TGG^CCA
MnlI 35 CCTC CCTC(7/6)
MscI 347 TGGCCA TGG^CCA
MslI 351 CATGTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 642 CAGCTG C[AC]G^C[GT]G
MvaI 506 CCAGG CC^[AT]GG
NlaIV 184 GGTTCC GG[ACGT]^[ACGT]CC
NsiI 362 ATGCAT ATGCA^T
PstI 399 CTGCAG CTGCA^G
PvuII 642 CAGCTG CAG^CTG
SacI 3 GAGCTC GAGCT^C
ScrFI 506 CCAGG CC^[ACGT]GG
SphI 360 GCATGC GCATG^C
XhoII 298 AGATCT [GA]^GATC[CT]
XmnI 676 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AfaI 104 GTAC GT^AC
601 GTAC
BbvI 398 GCTGC GCAGC(8/12)
514 GCTGC
BspMI 556 ACCTGC ACCTGC(4/8)
235 GCAGGT
Cac8I 360 GCATGC GC[ACGT]^[ACGT]GC
510 GCAAGC
DdeI 640 CTCAG C^T[ACGT]AG
719 CTAAG
DpnII 211 GATC ^GATC
299 GATC
Fnu4HI 398 GCTGC GC^[ACGT]GC
514 GCTGC
ItaI 398 GCTGC GC^[ACGT]GC
514 GCTGC
MboI 211 GATC ^GATC
299 GATC
NdeII 211 GATC ^GATC
299 GATC
NspI 216 GCATGT [GA]CATG^[CT]
360 GCATGC
RsaI 104 GTAC GT^AC
601 GTAC
Sau3AI 211 GATC ^GATC
299 GATC
SfaNI 122 GATGC GCATC(5/9)
250 GATGC
SfcI 375 CTACAG C^T[GA][CT]AG
399 CTGCAG
TseI 398 GCTGC G^C[AT]GC
514 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
#########################################
AluI HindIII HinfI MboII MseI
MwoI NlaIII TfiI Tru9I Tsp509I
TspRI