Restriction map for
pF1KB3336
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AcsI AflIII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BamHI BbeI BcgI BciVI
BlnI BsaWI BseAI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I CfoI ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HaeII
HhaI HinP1I HindIII HpaI KasI
KpnI KspI MfeI MluI MroI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tsp509I XbaI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 895 GACGTC GACGT^C
AflII 1044 CTTAAG C^TTAAG
AocI 315 CCTGAGG CC^T[ACGT]AGG
ApaI 1568 GGGCCC GGGCC^C
AspHI 876 GAGCTC G[AT]GC[AT]^C
AvaI 117 CTCGAG C^[CT]CG[GA]G
BalI 356 TGGCCA TGG^CCA
BbrPI 424 CACGTG CAC^GTG
BbvCI 750 CCTCAGC CCTCAGC(-5/-2)
BclI 791 TGATCA T^GATCA
BfaI 973 CTAG C^TAG
BfrI 1044 CTTAAG C^TTAAG
BglII 962 AGATCT A^GATCT
BlpI 873 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 873 GCTGAGC GC^T[ACGT]AGC
BsaAI 424 CACGTG [CT]AC^GT[GA]
BsaBI 354 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 430 GAGACC GGTCTC(1/5)
BsgI 1254 CTGCAC GTGCAG(16/14)
BsiHKAI 876 GAGCTC G[AT]GC[AT]^C
BsoBI 117 CTCGAG C^[CT]CG[GA]G
BspMI 1277 ACCTGC ACCTGC(4/8)
BsrFI 1348 GCCGGT [GA]^CCGG[CT]
BssSI 742 CTCGTG CACGAG(-5/-1)
Bsu36I 315 CCTGAGG CC^T[ACGT]AGG
CelII 873 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1348 GCCGGT [GA]^CCGG[CT]
DraIII 1645 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 315 CCTGAGG CC^T[ACGT]AGG
FbaI 791 TGATCA T^GATCA
HincII 301 GTTGAC GT[CT]^[GA]AC
HindII 301 GTTGAC GT[CT]^[GA]AC
MaeI 973 CTAG C^TAG
MamI 354 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 356 TGGCCA TGG^CCA
MscI 356 TGGCCA TGG^CCA
MslI 774 CATCTTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 814 CCATGG C^CATGG
NspI 103 ACATGC [GA]CATG^[CT]
PaeR7I 117 CTCGAG C^TCGAG
PmaCI 424 CACGTG CAC^GTG
PmlI 424 CACGTG CAC^GTG
SacI 876 GAGCTC GAGCT^C
SapI 1284 GCTCTTC GCTCTTC(1/4)
SexAI 1442 ACCTGGT A^CC[AT]GGT
TfiI 280 GATTC G^A[AT]TC
XcmI 520 CCAGGCCACACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 117 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 783 GTAC GT^AC
1140 GTAC
AlwI 264 GATCC GGATC(4/5)
575 GGATC
AspI 304 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
510 GACGCTGTC
BbsI 1168 GAAGAC GAAGAC(2/6)
1206 GTCTTC
BpuAI 1168 GAAGAC GAAGAC(2/6)
1206 GTCTTC
BsmBI 586 GAGACG CGTCTC(1/5)
893 GAGACG
BstYI 575 GGATCT [GA]^GATC[CT]
962 AGATCT
DsaI 724 CCGTGG C^C[GA][CT]GG
814 CCATGG
MaeII 425 ACGT A^CGT
896 ACGT
MflI 575 GGATCT [GA]^GATC[CT]
962 AGATCT
MseI 1045 TTAA T^TAA
1574 TTAA
PflFI 304 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
510 GACGCTGTC
RsaI 783 GTAC GT^AC
1140 GTAC
StuI 1127 AGGCCT AGG^CCT
1543 AGGCCT
Tru9I 1045 TTAA T^TAA
1574 TTAA
Tsp45I 310 GTCAC ^GT[GC]AC
997 GTGAC
Tth111I 304 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
510 GACGCTGTC
XhoII 575 GGATCT [GA]^GATC[CT]
962 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AcyI AluI AlwNI AvaII
BanI BanII BbvI BcnI BglI
BmyI BpmI BsaHI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraII EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI Hin1I HinfI HpaII HphI
ItaI Ksp632I MaeIII MboI MboII
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TseI TspRI TthHB8I
Van91I