Restriction map for
pF1KB3233
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# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbrPI
BcgI BclI BfrI BglII BlnI
BsaAI BsaBI BseAI BsiWI BspDI
BspEI BspLU11I BsrBI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstZ17I
Bsu36I ClaI DraI DrdI Eam1105I
Eco47III Eco81I EcoRV EcoT22I FauI
FbaI FseI FspI HincII HindII
HpaI KpnI KspI MamI MluI
MroI MvnI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
SacII SalI ScaI SfiI SfuI
SmlI SnaBI SpeI SphI SspI
SwaI Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AgeI 130 ACCGGT A^CCGGT
AvaI 872 CCCGGG C^[CT]CG[GA]G
BbeI 2038 GGCGCC GGCGC^C
BbvCI 787 GCTGAGG CCTCAGC(-5/-2)
BciVI 637 GGATAC GTATCC(6/5)
BglI 1382 GCCAAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2170 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2170 GCTGAGC GC^T[ACGT]AGC
BsaI 1808 GAGACC GGTCTC(1/5)
BsaWI 130 ACCGGT [AT]^CCGG[AT]
BsiEI 2333 CGGCCG CG[GA][CT]^CG
BsmBI 766 CGTCTC CGTCTC(1/5)
BsoBI 872 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1151 TGTACA T^GTACA
BspHI 1133 TCATGA T^CATGA
BsrFI 130 ACCGGT [GA]^CCGG[CT]
BsrGI 1151 TGTACA T^GTACA
BstYI 2096 GGATCT [GA]^GATC[CT]
CelII 2170 GCTGAGC GC^T[ACGT]AGC
Cfr10I 130 ACCGGT [GA]^CCGG[CT]
CpoI 2320 CGGTCCG CG^G[AT]CCG
DraIII 1201 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2333 CGGCCG C^GGCCG
EclXI 2333 CGGCCG C^GGCCG
Eco52I 2333 CGGCCG C^GGCCG
EcoRI 929 GAATTC G^AATTC
HaeII 2038 GGCGCC [GA]GCGC^[CT]
HindIII 1156 AAGCTT A^AGCTT
KasI 2038 GGCGCC G^GCGCC
MaeII 1220 ACGT A^CGT
MflI 2096 GGATCT [GA]^GATC[CT]
NarI 2038 GGCGCC GG^CGCC
PflMI 2160 CCAACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 130 ACCGGT A^CCGGT
RcaI 1133 TCATGA T^CATGA
RsrII 2320 CGGTCCG CG^G[AT]CCG
SacI 1549 GAGCTC GAGCT^C
SbfI 1812 CCTGCAGG CCTGCA^GG
SexAI 311 ACCTGGT A^CC[AT]GGT
SfoI 2038 GGCGCC GGC^GCC
SgrAI 129 CACCGGTG C[GA]^CCGG[CT]G
SmaI 872 CCCGGG CCC^GGG
Sse8387I 1812 CCTGCAGG CCTGCA^GG
SspBI 1151 TGTACA T^GTACA
StuI 168 AGGCCT AGG^CCT
Van91I 2160 CCAACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 2096 GGATCT [GA]^GATC[CT]
XmaCI 872 CCCGGG C^CCGGG
XmaI 872 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 909 GACGCC G[GA]^CG[CT]C
2038 GGCGCC
BalI 1328 TGGCCA TGG^CCA
1380 TGGCCA
BfaI 1162 CTAG C^TAG
2254 CTAG
BsaHI 909 GACGCC G[GA]^CG[CT]C
2038 GGCGCC
BsgI 1647 CTGCAC GTGCAG(16/14)
895 GTGCAG
BsmI 90 GAATGC GAATGC(1/-1)
1245 GAATGC
BspMI 1811 ACCTGC ACCTGC(4/8)
424 GCAGGT
BsrDI 51 CATTGC GCAATG(2/0)
1892 GCAATG
BstEII 1018 GGTAACC G^GT[ACGT]ACC
1059 GGTGACC
BstPI 1018 GGTAACC G^GT[ACGT]ACC
1059 GGTGACC
CfoI 1032 GCGC GCG^C
2039 GCGC
EarI 10 GAAGAG CTCTTC(1/4)
1425 GAAGAG
EcoNI 1278 CCTTATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1326 CCTGGCCAAGG
EcoO65I 1018 GGTAACC G^GT[ACGT]ACC
1059 GGTGACC
HhaI 1032 GCGC GCG^C
2039 GCGC
Hin1I 909 GACGCC G[GA]^CG[CT]C
2038 GGCGCC
HinP1I 1032 GCGC G^CGC
2039 GCGC
Ksp632I 10 GAAGAG CTCTTC(1/4)
1425 GAAGAG
MaeI 1162 CTAG C^TAG
2254 CTAG
MfeI 491 CAATTG C^AATTG
797 CAATTG
MluNI 1328 TGGCCA TGG^CCA
1380 TGGCCA
MscI 1328 TGGCCA TGG^CCA
1380 TGGCCA
MunI 491 CAATTG C^AATTG
797 CAATTG
NcoI 1082 CCATGG C^CATGG
1745 CCATGG
NspI 891 GCATGT [GA]CATG^[CT]
1982 ACATGC
PpuMI 751 AGGACCC [GA]G^G[AT]CC[CT]
979 GGGACCC
PstI 1235 CTGCAG CTGCA^G
1813 CTGCAG
SapI 10 GAAGAGC GCTCTTC(1/4)
1425 GAAGAGC
XcmI 703 CCAGATGACGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1745 CCATGGCTGGACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BanI
BanII BbsI BbvI BcnI BmyI
BpmI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
Cac8I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
HinfI HpaII HphI ItaI MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I