Restriction map for
pF1KB3200
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AgeI Alw44I AocI
Aor51HI ApaLI AscI Asp718I AviII
AvrII BamHI BbrPI BbvCI BcgI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaI BsaWI BseAI
BsiWI BspDI BspEI BsrBI BssHII
Bst1107I BstEII BstPI BstUI BstZ17I
Bsu36I CelII ClaI CpoI DrdI
DsaI Eco47III Eco81I EcoNI EcoO65I
FseI FspI HindIII HpaI KpnI
KspI MfeI MluI MroI MunI
MvnI NcoI NdeI NheI NotI
NruI PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SwaI Van91I XhoI
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# ONE CUT ENZYMES ( 71 enzymes) #
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AcyI 40 GGCGCC G[GA]^CG[CT]C
AflIII 1590 ACATGT A^C[GA][CT]GT
AhdI 320 GACAGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 671 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 57 GGGCCC GGGCC^C
AspEI 320 GACAGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 709 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 53 CCCGGG C^[CT]CG[GA]G
BbeI 40 GGCGCC GGCGC^C
BciVI 807 GGATAC GTATCC(6/5)
BclI 569 TGATCA T^GATCA
BsaBI 1632 GATTGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 40 GGCGCC G[GA]^CG[CT]C
BseRI 957 CTCCTC GAGGAG(10/8)
BsgI 967 GTGCAG GTGCAG(16/14)
BsiEI 65 CGGCCG CG[GA][CT]^CG
BsmFI 577 GTCCC GGGAC(10/14)
BsoBI 53 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1194 TGTACA T^GTACA
BspHI 102 TCATGA T^CATGA
BspLU11I 1590 ACATGT A^CATGT
BspMI 1809 ACCTGC ACCTGC(4/8)
BsrDI 717 CATTGC GCAATG(2/0)
BsrGI 1194 TGTACA T^GTACA
BstAPI 556 GCAAAAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1942 TTCGAA TT^CGAA
BstYI 1200 GGATCT [GA]^GATC[CT]
CfoI 41 GCGC GCG^C
DraI 424 TTTAAA TTT^AAA
DraIII 853 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 65 CGGCCG C^GGCCG
Eam1105I 320 GACAGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 65 CGGCCG C^GGCCG
Eco52I 65 CGGCCG C^GGCCG
EcoRV 630 GATATC GAT^ATC
EcoT22I 137 ATGCAT ATGCA^T
FbaI 569 TGATCA T^GATCA
HaeII 40 GGCGCC [GA]GCGC^[CT]
HgaI 363 GCGTC GACGC(5/10)
HhaI 41 GCGC GCG^C
Hin1I 40 GGCGCC G[GA]^CG[CT]C
HinP1I 41 GCGC G^CGC
HincII 1035 GTTGAC GT[CT]^[GA]AC
HindII 1035 GTTGAC GT[CT]^[GA]AC
KasI 40 GGCGCC G^GCGCC
MaeII 1620 ACGT A^CGT
MamI 1632 GATTGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1200 GGATCT [GA]^GATC[CT]
MslI 1296 CACGGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 40 GGCGCC GG^CGCC
NsiI 137 ATGCAT ATGCA^T
NspI 1590 ACATGT [GA]CATG^[CT]
NspV 1942 TTCGAA TT^CGAA
PflFI 709 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 671 CAGCTG CAG^CTG
RcaI 102 TCATGA T^CATGA
SacI 1858 GAGCTC GAGCT^C
SapI 1401 GAAGAGC GCTCTTC(1/4)
SfoI 40 GGCGCC GGC^GCC
SfuI 1942 TTCGAA TT^CGAA
SmaI 53 CCCGGG CCC^GGG
SspBI 1194 TGTACA T^GTACA
SspI 1894 AATATT AAT^ATT
StuI 839 AGGCCT AGG^CCT
TaqI 1943 TCGA T^CGA
Tth111I 709 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1943 TCGA T^CGA
XbaI 693 TCTAGA T^CTAGA
XhoII 1200 GGATCT [GA]^GATC[CT]
XmaCI 53 CCCGGG C^CCGGG
XmaI 53 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AlwI 388 GATCC GGATC(4/5)
1200 GGATC
AseI 658 ATTAAT AT^TAAT
1372 ATTAAT
AsnI 658 ATTAAT AT^TAAT
1372 ATTAAT
AspHI 1043 GTGCTC G[AT]GC[AT]^C
1858 GAGCTC
AvaII 1012 GGACC G^G[AT]CC
1800 GGACC
BalI 624 TGGCCA TGG^CCA
1250 TGGCCA
BanI 40 GGCGCC G^G[CT][GA]CC
247 GGTGCC
BcnI 53 CCCGG CC^[GC]GG
54 CCGGG
BpmI 232 CTGGAG CTGGAG(16/14)
1481 CTGGAG
BsiHKAI 1043 GTGCTC G[AT]GC[AT]^C
1858 GAGCTC
BsmBI 31 CGTCTC CGTCTC(1/5)
364 CGTCTC
BsmI 237 GAATGC GAATGC(1/-1)
305 GAATGC
BsrI 1999 ACTGG ACTGG(1/-1)
1641 CCAGT
BssSI 34 CTCGTG CACGAG(-5/-1)
1535 CTCGTG
BstXI 627 CCAGATATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1641 CCAGTCTTCTGG
DraII 56 GGGGCCC [GA]G^G[ACGT]CC[CT]
57 GGGCCCT
EcoO109I 56 GGGGCCC [GA]G^G[ACGT]CC[CT]
57 GGGCCCT
EcoRI 1520 GAATTC G^AATTC
1792 GAATTC
EcoT14I 1178 CCAAGG C^C[AT][AT]GG
1497 CCTTGG
FauI 71 CCCGC CCCGC(4/6)
129 GCGGG
MluNI 624 TGGCCA TGG^CCA
1250 TGGCCA
MscI 624 TGGCCA TGG^CCA
1250 TGGCCA
MspA1I 671 CAGCTG C[AC]G^C[GT]G
1424 CCGCTG
NciI 53 CCCGG CC^[GC]GG
54 CCGGG
PleI 595 GACTC GAGTC(4/5)
489 GAGTC
PshBI 658 ATTAAT AT^TAAT
1372 ATTAAT
PstI 20 CTGCAG CTGCA^G
530 CTGCAG
SfcI 20 CTGCAG C^T[GA][CT]AG
530 CTGCAG
StyI 1178 CCAAGG C^C[AT][AT]GG
1497 CCTTGG
XcmI 188 CCAACTCCTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1641 CCAGTCTTCTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
Asp700I BanII BbsI BbvI BfaI
BmyI BpuAI BsaJI BsiYI BslI
BsmAI Bsp1286I BsrFI BssKI BstF5I
BstNI Cac8I Cfr10I Cfr13I DdeI
DpnII EaeI EarI EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MseI MspI
MvaI MwoI NaeI NdeII NgoMIV
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XmnI