Restriction map for
pF1KB3110
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AgeI AhdI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AspHI AspI AviII
AvrII BamHI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BsaBI
BsaI BsaWI BseAI BsiHKAI BsiWI
BsmFI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrFI BsrGI BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Cfr10I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI HphI MamI
MfeI MluI MroI MseI MslI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SalI SbfI
ScaI SexAI SfaNI SfiI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tru9I Tsp509I Tth111I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AflIII 739 ACGTGT A^C[GA][CT]GT
AlwI 684 GATCC GGATC(4/5)
AocI 676 CCTGAGG CC^T[ACGT]AGG
ApaI 62 GGGCCC GGGCC^C
AvaII 714 GGACC G^G[AT]CC
BbeI 166 GGCGCC GGCGC^C
BbsI 882 GAAGAC GAAGAC(2/6)
BfaI 775 CTAG C^TAG
BglI 260 GCCGCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 664 CTGGAG CTGGAG(16/14)
BpuAI 882 GAAGAC GAAGAC(2/6)
BsgI 192 GTGCAG GTGCAG(16/14)
BsiEI 198 CGACCG CG[GA][CT]^CG
BsmAI 590 GAGAC GTCTC(1/5)
BsmBI 590 GAGACG CGTCTC(1/5)
BsrBI 421 CCGCTC CCGCTC(-3/-3)
BsrDI 39 GCAATG GCAATG(2/0)
BstAPI 78 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 683 AGATCC [GA]^GATC[CT]
Bsu36I 676 CCTGAGG CC^T[ACGT]AGG
DsaI 567 CCGCGG C^C[GA][CT]GG
EarI 669 GAAGAG CTCTTC(1/4)
Eco81I 676 CCTGAGG CC^T[ACGT]AGG
EcoNI 648 CCTGAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
KasI 166 GGCGCC G^GCGCC
Ksp632I 669 GAAGAG CTCTTC(1/4)
KspI 567 CCGCGG CCGC^GG
MaeI 775 CTAG C^TAG
MflI 683 AGATCC [GA]^GATC[CT]
NarI 166 GGCGCC GG^CGCC
PflMI 534 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 544 GACTC GAGTC(4/5)
PstI 85 CTGCAG CTGCA^G
PvuII 88 CAGCTG CAG^CTG
SacII 567 CCGCGG CCGC^GG
SapI 669 GAAGAGC GCTCTTC(1/4)
SfcI 85 CTGCAG C^T[GA][CT]AG
SfoI 166 GGCGCC GGC^GCC
TfiI 520 GAATC G^A[AT]TC
Van91I 534 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 683 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 107 GGTACC G^GTACC
619 GGTACC
AlwNI 79 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
88 CAGCTGCTG
Asp718I 107 GGTACC G^GTACC
619 GGTACC
BalI 606 TGGCCA TGG^CCA
734 TGGCCA
BbrPI 738 CACGTG CAC^GTG
766 CACGTG
BlpI 423 GCTCAGC GC^T[ACGT]AGC
466 GCTCAGC
Bpu1102I 423 GCTCAGC GC^T[ACGT]AGC
466 GCTCAGC
BsaAI 738 CACGTG [CT]AC^GT[GA]
766 CACGTG
BseRI 148 GAGGAG GAGGAG(10/8)
679 GAGGAG
CelII 423 GCTCAGC GC^T[ACGT]AGC
466 GCTCAGC
DpnII 294 GATC ^GATC
684 GATC
DraIII 286 CACGTCGTG CAC[ACGT][ACGT][ACGT]^GTG
367 CACGCCGTG
HinfI 520 GAATC G^A[ACGT]TC
544 GACTC
KpnI 107 GGTACC GGTAC^C
619 GGTACC
MaeIII 375 GTCAC ^GT[ACGT]AC
754 GTCAC
MboI 294 GATC ^GATC
684 GATC
MluNI 606 TGGCCA TGG^CCA
734 TGGCCA
MscI 606 TGGCCA TGG^CCA
734 TGGCCA
MspA1I 88 CAGCTG C[AC]G^C[GT]G
567 CCGCGG
NdeII 294 GATC ^GATC
684 GATC
NlaIII 222 CATG CATG^
840 CATG
NspI 221 ACATGC [GA]CATG^[CT]
839 ACATGC
PmaCI 738 CACGTG CAC^GTG
766 CACGTG
PmlI 738 CACGTG CAC^GTG
766 CACGTG
Sau3AI 294 GATC ^GATC
684 GATC
SmaI 162 CCCGGG CCC^GGG
229 CCCGGG
Tsp45I 375 GTCAC ^GT[GC]AC
754 GTCAC
XmaCI 162 CCCGGG C^CCGGG
229 CCCGGG
XmaI 162 CCCGGG C^CCGGG
229 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaI BanI BanII BbvI BcnI
BmyI BsaHI BsaJI BsiYI BslI
BsoBI Bsp1286I BspMI BsrI BssHII
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DraII EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HpaII ItaI MaeII
MboII MnlI MspI MvaI MvnI
MwoI NciI NlaIV RsaI Sau96I
ScrFI TaqI TseI TspRI TthHB8I