Restriction map for
pF1KB3064
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvrII BalI BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfaI
BfrI BglI BglII BlnI BsaBI
BsaHI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmBI BspDI BspEI
BspLU11I BspMI BsrBI BsrDI BsrFI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraII DraIII DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
FauI FbaI FseI HaeII HapII
HgaI Hin1I HincII HindII HpaI
HpaII HphI KasI KpnI KspI
MaeI MamI MfeI MluNI MroI
MscI MspI MunI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SpeI SphI Sse8387I SspI
StuI SwaI Tth111I Van91I XbaI
XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccII 1087 CGCG CG^CG
AcsI 232 GAATTT [GA]^AATT[CT]
AflIII 1086 ACGCGT A^C[GA][CT]GT
ApoI 232 GAATTT [GA]^AATT[CT]
AspHI 811 GAGCTC G[AT]GC[AT]^C
AvaI 335 CTCGAG C^[CT]CG[GA]G
AvaII 247 GGACC G^G[AT]CC
AviII 1131 TGCGCA TGC^GCA
BamHI 940 GGATCC G^GATCC
BanI 1173 GGCACC G^G[CT][GA]CC
BbvI 1193 GCAGC GCAGC(8/12)
BlpI 808 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 808 GCTGAGC GC^T[ACGT]AGC
BsiHKAI 811 GAGCTC G[AT]GC[AT]^C
BsmFI 9 GTCCC GGGAC(10/14)
BsoBI 335 CTCGAG C^[CT]CG[GA]G
Bsp1407I 456 TGTACA T^GTACA
BspHI 1008 TCATGA T^CATGA
BsrGI 456 TGTACA T^GTACA
BssSI 1000 CTCGTG CACGAG(-5/-1)
BstUI 1087 CGCG CG^CG
BstXI 271 CCAAAAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 808 GCTGAGC GC^T[ACGT]AGC
CfoI 1132 GCGC GCG^C
DraI 91 TTTAAA TTT^AAA
DrdI 248 GACCTCTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 73 GAAGAG CTCTTC(1/4)
EcoT14I 1154 CCTTGG C^C[AT][AT]GG
FspI 1131 TGCGCA TGC^GCA
HhaI 1132 GCGC GCG^C
HinP1I 1132 GCGC G^CGC
HindIII 487 AAGCTT A^AGCTT
Ksp632I 73 GAAGAG CTCTTC(1/4)
MluI 1086 ACGCGT A^CGCGT
MvnI 1087 CGCG CG^CG
PaeR7I 335 CTCGAG C^TCGAG
PstI 506 CTGCAG CTGCA^G
PvuII 750 CAGCTG CAG^CTG
RcaI 1008 TCATGA T^CATGA
SacI 811 GAGCTC GAGCT^C
ScaI 976 AGTACT AGT^ACT
SnaBI 710 TACGTA TAC^GTA
SspBI 456 TGTACA T^GTACA
StyI 1154 CCTTGG C^C[AT][AT]GG
TseI 1193 GCAGC G^C[AT]GC
XhoI 335 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AciI 292 CCGC CCGC(-3/-1)
482 GCGG
AlwNI 449 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
890 CAGGATCTG
Asp700I 277 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
681 GAAGACATTC
BanII 811 GAGCTC G[GA]GC[CT]^C
851 GGGCTC
BbsI 681 GAAGAC GAAGAC(2/6)
1119 GAAGAC
BmyI 811 GAGCTC G[AGT]GC[ACT]^C
851 GGGCTC
BpmI 854 CTCCAG CTGGAG(16/14)
416 CTGGAG
BpuAI 681 GAAGAC GAAGAC(2/6)
1119 GAAGAC
BsaAI 710 TACGTA [CT]AC^GT[GA]
1022 TACGTG
BsaI 266 GAGACC GGTCTC(1/5)
369 GGTCTC
BsiYI 1038 CCATTCTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1164 CCACAAGACGG
BslI 1038 CCATTCTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1164 CCACAAGACGG
Bsp1286I 811 GAGCTC G[AGT]GC[ACT]^C
851 GGGCTC
BssKI 848 CCTGG ^CC[ACGT]GG
919 CCTGG
BstNI 848 CCTGG CC^[AT]GG
919 CCTGG
BstYI 892 GGATCT [GA]^GATC[CT]
940 GGATCC
Cac8I 485 GCAAGC GC[ACGT]^[ACGT]GC
748 GCCAGC
Cfr13I 247 GGACC G^G[ACGT]CC
922 GGGCC
DdeI 809 CTGAG C^T[ACGT]AG
896 CTGAG
EaeI 567 CGGCCA [CT]^GGCC[GA]
721 CGGCCA
EcoRII 848 CCTGG ^CC[AT]GG
919 CCTGG
Fnu4HI 482 GCGGC GC^[ACGT]GC
1193 GCAGC
ItaI 482 GCGGC GC^[ACGT]GC
1193 GCAGC
MflI 892 GGATCT [GA]^GATC[CT]
940 GGATCC
MslI 565 CACGGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
823 CATGAGCATG
MspA1I 480 CAGCGG C[AC]G^C[GT]G
750 CAGCTG
MvaI 848 CCTGG CC^[AT]GG
919 CCTGG
NspI 828 GCATGT [GA]CATG^[CT]
983 GCATGT
Sau96I 247 GGACC G^G[ACGT]CC
922 GGGCC
ScrFI 848 CCTGG CC^[ACGT]GG
919 CCTGG
SfcI 41 CTATAG C^T[GA][CT]AG
506 CTGCAG
SmlI 335 CTCGAG C^T[CT][GA]AG
630 CTCAAG
TfiI 205 GAATC G^A[AT]TC
282 GATTC
TspRI 360 CAGTG CA[GC]TG[ACGT][ACGT]^
1188 CACTG
XhoII 892 GGATCT [GA]^GATC[CT]
940 GGATCC
XmnI 277 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
681 GAAGACATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AfaI AluI AlwI BsaJI BsmAI
BsmI BsrI BstF5I DpnII EcoT22I
FokI HaeIII HinfI MaeII MaeIII
MboI MboII MnlI MseI MwoI
NdeII NlaIII NlaIV NsiI PleI
RsaI Sau3AI SfaNI TaqI Tru9I
Tsp45I Tsp509I TthHB8I