Restriction map for
pF1KB2260
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# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp718I AspEI BamHI BbrPI
BbsI BcgI BciVI BclI BfrI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaI BseAI BsiWI BsmBI BspDI
BspEI BspLU11I BsrBI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI DraI Eam1105I Eco47III
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HpaI KpnI KspI MamI
MfeI MluI MroI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacII SapI SfiI SfuI SgrAI
SnaBI SpeI SspI StuI SwaI
XbaI XhoI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 1774 GTCGAC GT^[AC][GT]AC
AcsI 2217 AAATTT [GA]^AATT[CT]
AflIII 2363 ACGTGT A^C[GA][CT]GT
Alw44I 1759 GTGCAC G^TGCAC
AocI 439 CCTGAGG CC^T[ACGT]AGG
ApaLI 1759 GTGCAC G^TGCAC
ApoI 2217 AAATTT [GA]^AATT[CT]
Asp700I 1887 GAATGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 511 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2085 TGCGCA TGC^GCA
AvrII 963 CCTAGG C^CTAGG
BbeI 1549 GGCGCC GGCGC^C
BlnI 963 CCTAGG C^CTAGG
BsaWI 116 ACCGGA [AT]^CCGG[AT]
BsmAI 1000 GTCTC GTCTC(1/5)
BsmI 1878 GAATGC GAATGC(1/-1)
Bsp1407I 2175 TGTACA T^GTACA
BspHI 518 TCATGA T^CATGA
BsrGI 2175 TGTACA T^GTACA
BssSI 1466 CTCGTG CACGAG(-5/-1)
BstAPI 2605 GCAGAGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 439 CCTGAGG CC^T[ACGT]AGG
DraIII 283 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2437 GACGAGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 439 CCTGAGG CC^T[ACGT]AGG
FspI 2085 TGCGCA TGC^GCA
HaeII 1549 GGCGCC [GA]GCGC^[CT]
HincII 1774 GTCGAC GT[CT]^[GA]AC
HindII 1774 GTCGAC GT[CT]^[GA]AC
HindIII 1732 AAGCTT A^AGCTT
KasI 1549 GGCGCC G^GCGCC
MaeII 2363 ACGT A^CGT
MseI 1814 TTAA T^TAA
NaeI 2483 GCCGGC GCC^GGC
NarI 1549 GGCGCC GG^CGCC
NgoMIV 2483 GCCGGC G^CCGGC
PflFI 511 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 569 GACTCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 518 TCATGA T^CATGA
SacI 541 GAGCTC GAGCT^C
SalI 1774 GTCGAC G^TCGAC
SbfI 1617 CCTGCAGG CCTGCA^GG
ScaI 254 AGTACT AGT^ACT
SexAI 284 ACCTGGT A^CC[AT]GGT
SfoI 1549 GGCGCC GGC^GCC
Sse8387I 1617 CCTGCAGG CCTGCA^GG
SspBI 2175 TGTACA T^GTACA
Tru9I 1814 TTAA T^TAA
Tth111I 511 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 1887 GAATGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AccII 2195 CGCG CG^CG
2457 CGCG
AcyI 1549 GGCGCC G[GA]^CG[CT]C
1624 GGCGTC
AlwI 79 GATCC GGATC(4/5)
267 GGATC
AlwNI 150 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
733 CAGGAGCTG
AspHI 541 GAGCTC G[AT]GC[AT]^C
1759 GTGCAC
BbvCI 1338 CCTCAGC CCTCAGC(-5/-2)
828 GCTGAGG
BglII 1788 AGATCT A^GATCT
1853 AGATCT
BsaHI 1549 GGCGCC G[GA]^CG[CT]C
1624 GGCGTC
BsiHKAI 541 GAGCTC G[AT]GC[AT]^C
1759 GTGCAC
BsrDI 1299 CATTGC GCAATG(2/0)
428 GCAATG
BstUI 2195 CGCG CG^CG
2457 CGCG
BstXI 1144 CCAGCCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2415 CCAGCCCTTTGG
EagI 1017 CGGCCG C^GGCCG
2503 CGGCCG
EclXI 1017 CGGCCG C^GGCCG
2503 CGGCCG
Eco52I 1017 CGGCCG C^GGCCG
2503 CGGCCG
Hin1I 1549 GGCGCC G[GA]^CG[CT]C
1624 GGCGTC
MslI 1762 CACCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2331 CATCCTCATG
MvnI 2195 CGCG CG^CG
2457 CGCG
NcoI 473 CCATGG C^CATGG
929 CCATGG
SmlI 1784 CTCAAG C^T[CT][GA]AG
1924 CTCAAG
TfiI 163 GATTC G^A[AT]TC
1473 GAATC
Tsp509I 1111 AATT ^AATT
2218 AATT
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# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AfaI AluI ApaI AvaI
AvaII BalI BanI BanII BbvI
BcnI BfaI BglI BmyI BpmI
BsaJI BseRI BsgI BsiEI BsiYI
BslI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SphI StyI TaqI
TseI Tsp45I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI