Restriction map for
pF1KB1529
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# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII AccIII AclI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
AspI AviII BbeI BcgI BglI
BsaBI BseAI BsiEI BsiWI BsmBI
BspEI BspHI BspLU11I BsrBI BssHII
BssSI Bst1107I BstAPI BstBI BstZ17I
CpoI DraIII EagI EclXI Eco47III
Eco52I EcoRV FseI FspI HpaI
KasI MamI MluI MroI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NspV PacI PflFI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SbfI ScaI
SexAI SfiI SfoI SfuI SmaI
SnaBI SpeI Sse8387I SwaI Tth111I
XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 66 enzymes) #
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Acc65I 763 GGTACC G^GTACC
AccI 2223 GTCTAC GT^[AC][GT]AC
AcyI 419 GACGCC G[GA]^CG[CT]C
AflII 2539 CTTAAG C^TTAAG
AflIII 3007 ACGTGT A^C[GA][CT]GT
AgeI 1191 ACCGGT A^CCGGT
AhdI 1253 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1787 CCTCAGG CC^T[ACGT]AGG
Asp700I 3478 GAAGTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 763 GGTACC G^GTACC
AspEI 1253 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 2664 GAGCAC G[AT]GC[AT]^C
AvaI 3765 CTCGAG C^[CT]CG[GA]G
BanII 1230 GGGCTC G[GA]GC[CT]^C
BbrPI 3006 CACGTG CAC^GTG
BclI 3161 TGATCA T^GATCA
BfrI 2539 CTTAAG C^TTAAG
BlpI 853 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 853 GCTCAGC GC^T[ACGT]AGC
BsaAI 3006 CACGTG [CT]AC^GT[GA]
BsaHI 419 GACGCC G[GA]^CG[CT]C
BsaWI 1191 ACCGGT [AT]^CCGG[AT]
BsgI 3579 GTGCAG GTGCAG(16/14)
BsiHKAI 2664 GAGCAC G[AT]GC[AT]^C
BsoBI 3765 CTCGAG C^[CT]CG[GA]G
Bsp1407I 3530 TGTACA T^GTACA
BspDI 3613 ATCGAT AT^CGAT
BsrFI 1191 ACCGGT [GA]^CCGG[CT]
BsrGI 3530 TGTACA T^GTACA
BstEII 3537 GGTAACC G^GT[ACGT]ACC
BstPI 3537 GGTAACC G^GT[ACGT]ACC
Bsu36I 1787 CCTCAGG CC^T[ACGT]AGG
CelII 853 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1191 ACCGGT [GA]^CCGG[CT]
ClaI 3613 ATCGAT AT^CGAT
DrdI 2214 GACTGCTGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 41 CCGCGG C^C[GA][CT]GG
Eam1105I 1253 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1787 CCTCAGG CC^T[ACGT]AGG
EcoO65I 3537 GGTAACC G^GT[ACGT]ACC
EcoT22I 3380 ATGCAT ATGCA^T
FbaI 3161 TGATCA T^GATCA
HaeII 3 GGCGCT [GA]GCGC^[CT]
Hin1I 419 GACGCC G[GA]^CG[CT]C
HincII 1941 GTCAAC GT[CT]^[GA]AC
HindII 1941 GTCAAC GT[CT]^[GA]AC
HindIII 1668 AAGCTT A^AGCTT
KpnI 763 GGTACC GGTAC^C
KspI 41 CCGCGG CCGC^GG
MfeI 809 CAATTG C^AATTG
MunI 809 CAATTG C^AATTG
NdeI 3383 CATATG CA^TATG
NsiI 3380 ATGCAT ATGCA^T
PaeR7I 3765 CTCGAG C^TCGAG
PinAI 1191 ACCGGT A^CCGGT
PmaCI 3006 CACGTG CAC^GTG
PmlI 3006 CACGTG CAC^GTG
SacII 41 CCGCGG CCGC^GG
SgrAI 1190 CACCGGTG C[GA]^CCGG[CT]G
SphI 308 GCATGC GCATG^C
SspBI 3530 TGTACA T^GTACA
StuI 120 AGGCCT AGG^CCT
Tsp45I 3648 GTCAC ^GT[GC]AC
XcmI 1305 CCAGGCTTCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3765 CTCGAG C^TCGAG
XmnI 3478 GAAGTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccII 42 CGCG CG^CG
54 CGCG
AvrII 285 CCTAGG C^CTAGG
687 CCTAGG
BbvCI 1242 CCTCAGC CCTCAGC(-5/-2)
2759 CCTCAGC
BciVI 711 GTATCC GTATCC(6/5)
963 GTATCC
BcnI 65 CCGGG CC^[GC]GG
3774 CCGGG
BlnI 285 CCTAGG C^CTAGG
687 CCTAGG
BsrDI 1520 CATTGC GCAATG(2/0)
1111 GCAATG
BstUI 42 CGCG CG^CG
54 CGCG
CfoI 4 GCGC GCG^C
53 GCGC
EcoRI 1678 GAATTC G^AATTC
2014 GAATTC
HhaI 4 GCGC GCG^C
53 GCGC
HinP1I 4 GCGC G^CGC
53 GCGC
MaeII 2180 ACGT A^CGT
3007 ACGT
MvnI 42 CGCG CG^CG
54 CGCG
NciI 65 CCGGG CC^[GC]GG
3774 CCGGG
SspI 2033 AATATT AAT^ATT
3506 AATATT
TfiI 2910 GATTC G^A[AT]TC
3452 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BalI BamHI
BanI BbsI BbvI BfaI BglII
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
Cfr13I DdeI DpnII DraI DraII
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
Tru9I TseI Tsp509I TspRI TthHB8I
Van91I XhoII