##################################
# NO CUT ENZYMES (   77 enzymes) #
##################################

Acc65I      AccIII      AclI        AgeI        AhdI        
Alw44I      AocI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AvrII       
BamHI       BciVI       BglII       BlnI        BsaBI       
BsaWI       BseAI       BsiEI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrGI       BssHII      
BssSI       Bst1107I    BstZ17I     Bsu36I      ClaI        
CpoI        DrdI        EagI        Eam1105I    EclXI       
Eco52I      Eco81I      EcoRI       EcoT22I     FseI        
HpaI        KpnI        KspI        MamI        MfeI        
MluI        MroI        MunI        NdeI        NotI        
NruI        NsiI        PacI        PflFI       PinAI       
PmeI        PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        ScaI        SgrAI       
SnaBI       SpeI        SspBI       SspI        SwaI        
Tth111I     XbaI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

AatII        5383  GACGTC               GACGT^C
AccI           50  GTCTAC               GT^[AC][GT]AC
AflII        1785  CTTAAG               C^TTAAG
AflIII         54  ACACGT               A^C[GA][CT]GT
Aor51HI      2895  AGCGCT               AGC^GCT
BbrPI          55  CACGTG               CAC^GTG
BclI         1400  TGATCA               T^GATCA
BfrI         1785  CTTAAG               C^TTAAG
BsiWI        4446  CGTACG               C^GTACG
BsmI          235  GAATGC               GAATGC(1/-1)
BstBI        1369  TTCGAA               TT^CGAA
BstEII       2136  GGTCACC              G^GT[ACGT]ACC
BstPI        2136  GGTCACC              G^GT[ACGT]ACC
BstYI        5175  GGATCT               [GA]^GATC[CT]
DraI         5118  TTTAAA               TTT^AAA
DraIII       1651  CACCAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III     2895  AGCGCT               AGC^GCT
EcoO65I      2136  GGTCACC              G^GT[ACGT]ACC
EcoRV         619  GATATC               GAT^ATC
FbaI         1400  TGATCA               T^GATCA
HincII       1485  GTTGAC               GT[CT]^[GA]AC
HindII       1485  GTTGAC               GT[CT]^[GA]AC
MflI         5175  GGATCT               [GA]^GATC[CT]
NspV         1369  TTCGAA               TT^CGAA
PmaCI          55  CACGTG               CAC^GTG
PmlI           55  CACGTG               CAC^GTG
SbfI         1107  CCTGCAGG             CCTGCA^GG
SexAI        1652  ACCAGGT              A^CC[AT]GGT
SfuI         1369  TTCGAA               TT^CGAA
Sse8387I     1107  CCTGCAGG             CCTGCA^GG
XhoII        5175  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AviII        2768  TGCGCA               TGC^GCA
             5039  TGCGCA              
BbeI         2482  GGCGCC               GGCGC^C
             6131  GGCGCC              
BbvCI        1431  CCTCAGC              CCTCAGC(-5/-2)
             4474  GCTGAGG             
BsaAI          55  CACGTG               [CT]AC^GT[GA]
             4448  TACGTG              
BsmBI         648  GAGACG               CGTCTC(1/5)
             5381  GAGACG              
FspI         2768  TGCGCA               TGC^GCA
             5039  TGCGCA              
HgaI         2870  GACGC                GACGC(5/10)
             5249  GCGTC               
HindIII      2689  AAGCTT               A^AGCTT
             3202  AAGCTT              
KasI         2482  GGCGCC               G^GCGCC
             6131  GGCGCC              
MslI         4858  CATTATGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             5448  CATCACCATG          
NarI         2482  GGCGCC               GG^CGCC
             6131  GGCGCC              
NspI         4313  GCATGC               [GA]CATG^[CT]
             5906  GCATGC              
PshAI        2343  GACTGAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             5395  GACTCGGGTC          
SfiI         3630  GGCCCAGGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             4113  GGCCGAGCAGGCC       
SfoI         2482  GGCGCC               GGC^GCC
             6131  GGCGCC              
SphI         4313  GCATGC               GCATG^C
             5906  GCATGC              


#########################################
# 3 OR MORE CUT ENZYMES (  134 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AlwNI       ApaI        ApoI        
Asp700I     AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbsI        BbvI        BcgI        
BcnI        BfaI        BglI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaHI       BsaI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrBI       BsrDI       BsrFI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstUI       
BstXI       Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MluNI       MnlI        MscI        MseI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NciI        NcoI        NdeII       NgoMIV      NheI        
NlaIII      NlaIV       PaeR7I      PflMI       PleI        
PpuMI       PstI        PvuII       RsaI        SacI        
SapI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmaI        SmlI        StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XcmI        
XhoI        XmaCI       XmaI        XmnI