Restriction map for
pF1KA1995
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# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI AspEI
AviII AvrII BbrPI BbsI BciVI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaWI BseAI
BsiEI BsiWI BspDI BspEI BspHI
BsrBI BssHII BssSI Bst1107I BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
FseI FspI HindIII HpaI MfeI
MluI MroI MunI NdeI NheI
NotI NruI PacI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SalI SbfI SexAI
SfiI SgrAI SnaBI SpeI SphI
Sse8387I SwaI
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# ONE CUT ENZYMES ( 55 enzymes) #
###################################
Acc65I 464 GGTACC G^GTACC
AccI 2924 GTAGAC GT^[AC][GT]AC
AcyI 181 GGCGCC G[GA]^CG[CT]C
AflIII 650 ACATGT A^C[GA][CT]GT
Asp700I 1422 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 464 GGTACC G^GTACC
AspI 1641 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1769 TGGCCA TGG^CCA
BbeI 181 GGCGCC GGCGC^C
BbvCI 1431 CCTCAGC CCTCAGC(-5/-2)
BcgI 792 GCAAGGAATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1955 TGATCA T^GATCA
BsaBI 1999 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 181 GGCGCC G[GA]^CG[CT]C
BsmBI 625 GAGACG CGTCTC(1/5)
BsmI 2826 GCATTC GAATGC(1/-1)
Bsp1407I 1169 TGTACA T^GTACA
BspLU11I 650 ACATGT A^CATGT
BsrGI 1169 TGTACA T^GTACA
BstAPI 1009 GCACCCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2435 TTCGAA TT^CGAA
BstXI 501 CCATAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 255 GACCCGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 2824 ATGCAT ATGCA^T
FbaI 1955 TGATCA T^GATCA
Hin1I 181 GGCGCC G[GA]^CG[CT]C
KasI 181 GGCGCC G^GCGCC
KpnI 464 GGTACC GGTAC^C
KspI 177 CCGCGG CCGC^GG
MamI 1999 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1769 TGGCCA TGG^CCA
MscI 1769 TGGCCA TGG^CCA
NarI 181 GGCGCC GG^CGCC
NsiI 2824 ATGCAT ATGCA^T
NspV 2435 TTCGAA TT^CGAA
PaeR7I 81 CTCGAG C^TCGAG
PflFI 1641 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1077 CCAGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1932 GGGACCC [GA]G^G[AT]CC[CT]
SacII 177 CCGCGG CCGC^GG
SapI 2536 GAAGAGC GCTCTTC(1/4)
ScaI 827 AGTACT AGT^ACT
SfoI 181 GGCGCC GGC^GCC
SfuI 2435 TTCGAA TT^CGAA
SmaI 2250 CCCGGG CCC^GGG
SmlI 81 CTCGAG C^T[CT][GA]AG
SspBI 1169 TGTACA T^GTACA
StuI 1589 AGGCCT AGG^CCT
Tth111I 1641 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1077 CCAGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 906 TCTAGA T^CTAGA
XhoI 81 CTCGAG C^TCGAG
XmaCI 2250 CCCGGG C^CCGGG
XmaI 2250 CCCGGG C^CCGGG
XmnI 1422 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AspHI 661 GAGCTC G[AT]GC[AT]^C
2241 GAGCTC
AvaI 81 CTCGAG C^[CT]CG[GA]G
2250 CCCGGG
AvaII 1933 GGACC G^G[AT]CC
2133 GGACC
BamHI 873 GGATCC G^GATCC
2868 GGATCC
BanII 661 GAGCTC G[GA]GC[CT]^C
2241 GAGCTC
BpmI 656 CTCCAG CTGGAG(16/14)
2761 CTCCAG
BsiHKAI 661 GAGCTC G[AT]GC[AT]^C
2241 GAGCTC
BsoBI 81 CTCGAG C^[CT]CG[GA]G
2250 CCCGGG
BsrFI 118 GCCGGC [GA]^CCGG[CT]
1847 GCCGGC
Cfr10I 118 GCCGGC [GA]^CCGG[CT]
1847 GCCGGC
DraII 90 GGGGCCT [GA]G^G[ACGT]CC[CT]
1932 GGGACCC
EaeI 1769 TGGCCA [CT]^GGCC[GA]
2037 TGGCCG
EcoNI 737 CCTTAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
864 CCTTGACCAGG
EcoO109I 90 GGGGCCT [GA]G^G[ACGT]CC[CT]
1932 GGGACCC
EcoRV 697 GATATC GAT^ATC
2080 GATATC
FauI 74 GCGGG CCCGC(4/6)
2264 GCGGG
HaeII 97 AGCGCC [GA]GCGC^[CT]
181 GGCGCC
HincII 814 GTTGAC GT[CT]^[GA]AC
1638 GTTGAC
HindII 814 GTTGAC GT[CT]^[GA]AC
1638 GTTGAC
MslI 1173 CACTTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1371 CATGGGGGTG
NaeI 118 GCCGGC GCC^GGC
1847 GCCGGC
NgoMIV 118 GCCGGC G^CCGGC
1847 GCCGGC
PshAI 79 GACTCGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
983 GACCAAGGTC
SacI 661 GAGCTC GAGCT^C
2241 GAGCTC
SspI 386 AATATT AAT^ATT
541 AATATT
XcmI 161 CCATCCGCGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2381 CCACAGAGGCCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI BanI BbvI
BcnI BfaI BmyI BsaI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EarI EcoRI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII