Restriction map for
pF1KA1760
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# NO CUT ENZYMES ( 68 enzymes) #
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AccIII AcsI AflII AgeI ApoI
AscI AseI AsnI AviII BciVI
BclI BfrI BsaBI BseAI BsiWI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI Bst1107I BstBI BstZ17I
ClaI CpoI DraI EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI MamI MfeI
MroI MseI MunI NcoI NdeI
NotI NruI NsiI NspV PacI
PinAI PmeI PshAI PshBI PvuI
RcaI RsrII SalI SapI ScaI
SfiI SfuI SnaBI SpeI SspBI
SwaI Tru9I XbaI
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# ONE CUT ENZYMES ( 27 enzymes) #
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Acc65I 6313 GGTACC G^GTACC
AclI 3690 AACGTT AA^CGTT
AhdI 4009 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1219 GTGCAC G^TGCAC
ApaLI 1219 GTGCAC G^TGCAC
Asp700I 819 GAAGCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 6313 GGTACC G^GTACC
AspEI 4009 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 6564 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 6195 CCTAGG C^CTAGG
BamHI 2058 GGATCC G^GATCC
BlnI 6195 CCTAGG C^CTAGG
BsmI 1158 GAATGC GAATGC(1/-1)
DraIII 2746 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 4009 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
KpnI 6313 GGTACC GGTAC^C
MluI 3481 ACGCGT A^CGCGT
NheI 5101 GCTAGC G^CTAGC
PflFI 6564 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 3690 AACGTT AA^CGTT
SexAI 6582 ACCAGGT A^CC[AT]GGT
SgrAI 1658 CGCCGGTG C[GA]^CCGG[CT]G
SphI 1112 GCATGC GCATG^C
SspI 940 AATATT AAT^ATT
Tsp509I 3639 AATT ^AATT
Tth111I 6564 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 819 GAAGCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
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AccI 583 GTCTAC GT^[AC][GT]AC
5275 GTCTAC
Aor51HI 74 AGCGCT AGC^GCT
255 AGCGCT
BbrPI 4648 CACGTG CAC^GTG
5009 CACGTG
BcgI 2168 CGACACCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3633 CGATGAAATTGC
BglII 1169 AGATCT A^GATCT
5678 AGATCT
BssHII 219 GCGCGC G^CGCGC
3487 GCGCGC
DrdI 4890 GACAGCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6108 GACCTATAAGTC
Eco47III 74 AGCGCT AGC^GCT
255 AGCGCT
NspI 1112 GCATGC [GA]CATG^[CT]
3734 ACATGC
PflMI 3588 CCACAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4815 CCAGCCCTTGG
PmaCI 4648 CACGTG CAC^GTG
5009 CACGTG
PmlI 4648 CACGTG CAC^GTG
5009 CACGTG
SbfI 1668 CCTGCAGG CCTGCA^GG
3672 CCTGCAGG
Sse8387I 1668 CCTGCAGG CCTGCA^GG
3672 CCTGCAGG
Van91I 3588 CCACAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4815 CCAGCCCTTGG
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# 3 OR MORE CUT ENZYMES ( 148 enzymes) #
#########################################
AatII AccII AciI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI AspHI AvaI AvaII BalI
BanI BanII BbeI BbsI BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BssSI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NdeII NgoMIV NlaIII NlaIV PaeR7I
PleI PpuMI PstI PvuII RsaI
SacI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI SmlI
StuI StyI TaqI TfiI TseI
Tsp45I TspRI TthHB8I XcmI XhoI
XhoII XmaCI XmaI