Restriction map for
pF1KA1700
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI AhdI AocI Aor51HI ApaI
AscI Asp718I AspEI AvrII BalI
BamHI BbeI BbrPI BcgI BciVI
BclI BcnI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaWI BseAI
BsiEI BsiWI Bsp1407I BspDI BspEI
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoT14I EcoT22I FbaI
FseI HpaI KasI KpnI KspI
MaeII MamI MfeI MluI MluNI
MroI MscI MunI NarI NciI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StyI SwaI
TfiI XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 65 enzymes) #
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AccII 1826 CGCG CG^CG
AcyI 1002 GACGCC G[GA]^CG[CT]C
AflII 1606 CTTAAG C^TTAAG
AflIII 791 ACATGT A^C[GA][CT]GT
Alw44I 724 GTGCAC G^TGCAC
AlwI 740 GGATC GGATC(4/5)
ApaLI 724 GTGCAC G^TGCAC
AseI 136 ATTAAT AT^TAAT
AsnI 136 ATTAAT AT^TAAT
Asp700I 336 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1774 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 604 CCCGAG C^[CT]CG[GA]G
AviII 1790 TGCGCA TGC^GCA
BanI 1477 GGCACC G^G[CT][GA]CC
BbsI 1171 GAAGAC GAAGAC(2/6)
BbvCI 1706 CCTCAGC CCTCAGC(-5/-2)
BfrI 1606 CTTAAG C^TTAAG
BpuAI 1171 GAAGAC GAAGAC(2/6)
BsaHI 1002 GACGCC G[GA]^CG[CT]C
BseRI 1260 CTCCTC GAGGAG(10/8)
BsmBI 1000 GAGACG CGTCTC(1/5)
BsoBI 604 CCCGAG C^[CT]CG[GA]G
BspHI 776 TCATGA T^CATGA
BspLU11I 791 ACATGT A^CATGT
BsrBI 747 CCGCTC CCGCTC(-3/-3)
BsrFI 92 GCCGGC [GA]^CCGG[CT]
BstAPI 1874 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1826 CGCG CG^CG
BstXI 1298 CCACTACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 740 GGATCT [GA]^GATC[CT]
Cfr10I 92 GCCGGC [GA]^CCGG[CT]
DraII 1489 AGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 1016 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 661 CCGTGG C^C[GA][CT]GG
EaeI 94 CGGCCA [CT]^GGCC[GA]
EcoNI 522 CCTCAACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1489 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRI 482 GAATTC G^AATTC
FspI 1790 TGCGCA TGC^GCA
HaeII 1134 GGCGCT [GA]GCGC^[CT]
HapII 93 CCGG C^CGG
HgaI 1002 GACGC GACGC(5/10)
Hin1I 1002 GACGCC G[GA]^CG[CT]C
HincII 229 GTTGAC GT[CT]^[GA]AC
HindII 229 GTTGAC GT[CT]^[GA]AC
HpaII 93 CCGG C^CGG
HphI 354 TCACC GGTGA(8/7)
MflI 740 GGATCT [GA]^GATC[CT]
MslI 1510 CATCGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 93 CCGG C^CGG
MvnI 1826 CGCG CG^CG
NaeI 92 GCCGGC GCC^GGC
NgoMIV 92 GCCGGC G^CCGGC
NspI 791 ACATGT [GA]CATG^[CT]
PflFI 1774 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1489 AGGTCCC [GA]G^G[AT]CC[CT]
PshBI 136 ATTAAT AT^TAAT
RcaI 776 TCATGA T^CATGA
SacI 199 GAGCTC GAGCT^C
StuI 1423 AGGCCT AGG^CCT
TaqI 1512 TCGA T^CGA
Tth111I 1774 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1512 TCGA T^CGA
XhoII 740 GGATCT [GA]^GATC[CT]
XmnI 336 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 316 GTAC GT^AC
1129 GTAC
AlwNI 1662 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1875 CAGAAGCTG
AspHI 199 GAGCTC G[AT]GC[AT]^C
724 GTGCAC
AvaII 1313 GGACC G^G[AT]CC
1490 GGTCC
BanII 199 GAGCTC G[GA]GC[CT]^C
1848 GAGCCC
BglI 1231 GCCAGCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1604 GCCTTAAGGGC
BsaI 1772 GAGACC GGTCTC(1/5)
1989 GGTCTC
BsiHKAI 199 GAGCTC G[AT]GC[AT]^C
724 GTGCAC
BsmI 210 GCATTC GAATGC(1/-1)
1452 GCATTC
CfoI 1135 GCGC GCG^C
1791 GCGC
DpnII 265 GATC ^GATC
741 GATC
EcoRV 1207 GATATC GAT^ATC
1624 GATATC
HhaI 1135 GCGC GCG^C
1791 GCGC
HinP1I 1135 GCGC G^CGC
1791 GCGC
HindIII 154 AAGCTT A^AGCTT
806 AAGCTT
MboI 265 GATC ^GATC
741 GATC
NdeII 265 GATC ^GATC
741 GATC
PstI 1411 CTGCAG CTGCA^G
1749 CTGCAG
RsaI 316 GTAC GT^AC
1129 GTAC
SapI 336 GAAGAGC GCTCTTC(1/4)
1930 GAAGAGC
Sau3AI 265 GATC ^GATC
741 GATC
Tsp45I 1811 GTGAC ^GT[GC]AC
1923 GTCAC
XcmI 6 CCATGAGATGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1884 CCAAATGGAATTTGG
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# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AcsI AluI ApoI BbvI
BfaI BmyI BpmI BsaJI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI EarI EcoRII
FauI Fnu4HI FokI HaeIII HinfI
ItaI Ksp632I MaeI MaeIII MboII
MnlI MseI MspA1I MvaI MwoI
NlaIII NlaIV PflMI PleI PvuII
Sau96I ScrFI SfaNI SfcI SmlI
Tru9I TseI Tsp509I TspRI Van91I