Restriction map for
pF1KA1579
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I AlwI Aor51HI
ApaLI AscI Asp718I AspEI AspI
AvaI AviII AvrII BamHI BbrPI
BbsI BcgI BciVI BclI BfrI
BglI BglII BlnI BpuAI BsaAI
BsaI BsaWI BseAI BsiEI BsiWI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrFI BsrGI BssHII BstBI
BstEII BstPI BstYI Cfr10I ClaI
CpoI DpnII DraI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HgaI HpaI KpnI
KspI MboI MfeI MflI MluI
MroI MunI NaeI NcoI NdeI
NdeII NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI RsrII
SacII SalI Sau3AI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI StuI StyI SwaI TaqI
Tth111I TthHB8I XbaI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AcyI 118 GGCGCC G[GA]^CG[CT]C
AflIII 972 ACGTGT A^C[GA][CT]GT
AocI 151 CCTGAGG CC^T[ACGT]AGG
ApaI 93 GGGCCC GGGCC^C
AseI 1224 ATTAAT AT^TAAT
AsnI 1224 ATTAAT AT^TAAT
Asp700I 478 GAACTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 537 TGGCCA TGG^CCA
BanI 118 GGCGCC G^G[CT][GA]CC
BbeI 118 GGCGCC GGCGC^C
BbvCI 98 CCTCAGC CCTCAGC(-5/-2)
BlpI 192 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 192 GCTGAGC GC^T[ACGT]AGC
BsaBI 655 GATGTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 118 GGCGCC G[GA]^CG[CT]C
BseRI 154 GAGGAG GAGGAG(10/8)
BsmAI 17 GAGAC GTCTC(1/5)
BsmBI 17 GAGACG CGTCTC(1/5)
BspHI 255 TCATGA T^CATGA
BspMI 224 ACCTGC ACCTGC(4/8)
BssSI 112 CACGAG CACGAG(-5/-1)
Bst1107I 2029 GTATAC GTA^TAC
BstXI 1077 CCAGTTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2029 GTATAC GTA^TAC
Bsu36I 151 CCTGAGG CC^T[ACGT]AGG
CelII 192 GCTGAGC GC^T[ACGT]AGC
DrdI 394 GACTTAATAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1499 CCACGG C^C[GA][CT]GG
EaeI 537 TGGCCA [CT]^GGCC[GA]
Eco81I 151 CCTGAGG CC^T[ACGT]AGG
EcoNI 225 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I 118 GGCGCC G[GA]^CG[CT]C
HincII 1966 GTTGAC GT[CT]^[GA]AC
HindII 1966 GTTGAC GT[CT]^[GA]AC
HindIII 1575 AAGCTT A^AGCTT
KasI 118 GGCGCC G^GCGCC
MamI 655 GATGTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 537 TGGCCA TGG^CCA
MscI 537 TGGCCA TGG^CCA
MslI 1348 CATCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 118 GGCGCC GG^CGCC
NspI 1418 GCATGT [GA]CATG^[CT]
PshBI 1224 ATTAAT AT^TAAT
PstI 172 CTGCAG CTGCA^G
RcaI 255 TCATGA T^CATGA
SacI 676 GAGCTC GAGCT^C
SapI 736 GAAGAGC GCTCTTC(1/4)
SfoI 118 GGCGCC GGC^GCC
Tsp45I 719 GTGAC ^GT[GC]AC
XcmI 1499 CCACGGTGGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 478 GAACTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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AccI 614 GTAGAC GT^[AC][GT]AC
2029 GTATAC
AlwNI 237 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
1637 CAGGGGCTG
AspHI 676 GAGCTC G[AT]GC[AT]^C
920 GTGCTC
AvaII 126 GGACC G^G[AT]CC
861 GGTCC
BpmI 601 CTGGAG CTGGAG(16/14)
926 CTGGAG
BsgI 145 CTGCAC GTGCAG(16/14)
1301 CTGCAC
BsiHKAI 676 GAGCTC G[AT]GC[AT]^C
920 GTGCTC
BsmFI 1344 GGGAC GGGAC(10/14)
1587 GGGAC
BsmI 1799 GCATTC GAATGC(1/-1)
1974 GCATTC
BsrDI 1446 CATTGC GCAATG(2/0)
1854 CATTGC
BstAPI 174 GCAGCGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
634 GCACTCTTTGC
DraII 92 AGGGCCC [GA]G^G[ACGT]CC[CT]
93 GGGCCCC
EcoO109I 92 AGGGCCC [GA]G^G[ACGT]CC[CT]
93 GGGCCCC
HaeII 118 GGCGCC [GA]GCGC^[CT]
930 AGCGCC
HphI 216 GGTGA GGTGA(8/7)
1876 GGTGA
PflMI 1867 CCACCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1942 CCAGAAGGTGG
PvuII 1283 CAGCTG CAG^CTG
1406 CAGCTG
Van91I 1867 CCACCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1942 CCAGAAGGTGG
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# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApoI BanII BbvI BcnI BfaI
BmyI BsaJI BsiYI BslI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI EarI
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI Ksp632I MaeI MaeII MaeIII
MboII MnlI MseI MspA1I MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV RsaI Sau96I ScrFI SfaNI
SfcI SspI TfiI Tru9I TseI
Tsp509I TspRI