Restriction map for
pF1KA1539
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII AccII AclI AcsI AflIII
AgeI AhdI Alw44I Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AspI AviII BbsI BbvCI
BcgI BclI BglI BglII BpuAI
BsaBI BsaI BsiEI BsiWI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstUI BstXI BstZ17I
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoT22I FbaI
FseI FspI HgaI HincII HindII
HindIII HpaI KspI MamI MfeI
MluI MslI MunI MvnI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SfiI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 172 GGTACC G^GTACC
AccI 139 GTCTAC GT^[AC][GT]AC
AcyI 791 GGCGCC G[GA]^CG[CT]C
AflII 1488 CTTAAG C^TTAAG
AocI 1458 CCTCAGG CC^T[ACGT]AGG
Asp718I 172 GGTACC G^GTACC
AvrII 637 CCTAGG C^CTAGG
BamHI 799 GGATCC G^GATCC
BbeI 791 GGCGCC GGCGC^C
BbrPI 7 CACGTG CAC^GTG
BciVI 148 GTATCC GTATCC(6/5)
BfrI 1488 CTTAAG C^TTAAG
BlnI 637 CCTAGG C^CTAGG
BsaAI 7 CACGTG [CT]AC^GT[GA]
BsaHI 791 GGCGCC G[GA]^CG[CT]C
BsmBI 23 CGTCTC CGTCTC(1/5)
BspMI 863 ACCTGC ACCTGC(4/8)
BsrBI 1476 CCGCTC CCGCTC(-3/-3)
BstBI 962 TTCGAA TT^CGAA
Bsu36I 1458 CCTCAGG CC^T[ACGT]AGG
Eco81I 1458 CCTCAGG CC^T[ACGT]AGG
EcoRV 1504 GATATC GAT^ATC
HaeII 791 GGCGCC [GA]GCGC^[CT]
Hin1I 791 GGCGCC G[GA]^CG[CT]C
KasI 791 GGCGCC G^GCGCC
KpnI 172 GGTACC GGTAC^C
MspA1I 544 CAGCTG C[AC]G^C[GT]G
NarI 791 GGCGCC GG^CGCC
NcoI 914 CCATGG C^CATGG
NspI 1349 ACATGC [GA]CATG^[CT]
NspV 962 TTCGAA TT^CGAA
PmaCI 7 CACGTG CAC^GTG
PmlI 7 CACGTG CAC^GTG
PstI 1564 CTGCAG CTGCA^G
PvuII 544 CAGCTG CAG^CTG
SacI 1470 GAGCTC GAGCT^C
SexAI 143 ACCAGGT A^CC[AT]GGT
SfoI 791 GGCGCC GGC^GCC
SfuI 962 TTCGAA TT^CGAA
SmlI 1488 CTTAAG C^T[CT][GA]AG
StuI 984 AGGCCT AGG^CCT
TaqI 963 TCGA T^CGA
TfiI 1060 GAATC G^A[AT]TC
Tsp509I 1119 AATT ^AATT
TthHB8I 963 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccIII 971 TCCGGA T^CCGGA
1466 TCCGGA
AfaI 173 GTAC GT^AC
1037 GTAC
AlwNI 471 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
602 CAGTGGCTG
AspHI 676 GAGCAC G[AT]GC[AT]^C
1470 GAGCTC
BalI 338 TGGCCA TGG^CCA
1092 TGGCCA
BlpI 951 GCTGAGC GC^T[ACGT]AGC
990 GCTGAGC
Bpu1102I 951 GCTGAGC GC^T[ACGT]AGC
990 GCTGAGC
BsaWI 971 TCCGGA [AT]^CCGG[AT]
1466 TCCGGA
BseAI 971 TCCGGA T^CCGGA
1466 TCCGGA
BseRI 1438 CTCCTC GAGGAG(10/8)
1408 GAGGAG
BsiHKAI 676 GAGCAC G[AT]GC[AT]^C
1470 GAGCTC
BsmAI 473 GAGAC GTCTC(1/5)
24 GTCTC
BspEI 971 TCCGGA T^CCGGA
1466 TCCGGA
BsrDI 1064 CATTGC GCAATG(2/0)
617 GCAATG
CelII 951 GCTGAGC GC^T[ACGT]AGC
990 GCTGAGC
DsaI 360 CCGTGG C^C[GA][CT]GG
914 CCATGG
EaeI 338 TGGCCA [CT]^GGCC[GA]
1092 TGGCCA
EarI 237 GAAGAG CTCTTC(1/4)
769 GAAGAG
EcoNI 969 CCTCCGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1238 CCTTGGGGAGG
FauI 1475 CCCGC CCCGC(4/6)
1523 CCCGC
Ksp632I 237 GAAGAG CTCTTC(1/4)
769 GAAGAG
MluNI 338 TGGCCA TGG^CCA
1092 TGGCCA
MroI 971 TCCGGA T^CCGGA
1466 TCCGGA
MscI 338 TGGCCA TGG^CCA
1092 TGGCCA
MseI 1295 TTAA T^TAA
1489 TTAA
PpuMI 222 GGGTCCC [GA]G^G[AT]CC[CT]
505 GGGTCCC
PshAI 500 GACAGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
904 GACTGCGGTC
RsaI 173 GTAC GT^AC
1037 GTAC
SfaNI 835 GATGC GCATC(5/9)
1220 GCATC
SfcI 105 CTACAG C^T[GA][CT]AG
1564 CTGCAG
SmaI 133 CCCGGG CCC^GGG
227 CCCGGG
Tru9I 1295 TTAA T^TAA
1489 TTAA
XcmI 861 CCACCTGCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1301 CCAACCAGACTGTGG
XmaCI 133 CCCGGG C^CCGGG
227 CCCGGG
XmaI 133 CCCGGG C^CCGGG
227 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AluI AlwI ApaI AvaI
AvaII BanI BanII BbvI BcnI
BfaI BmyI BpmI BsaJI BsgI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
Sau3AI Sau96I ScrFI StyI TseI
Tsp45I TspRI Van91I XhoII