Restriction map for
pF1KA1485
##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AclI AflII AhdI Aor51HI AscI
AseI AsnI AspEI AspI BbrPI
BciVI BfrI BlpI Bpu1102I BsaBI
BsiWI Bsp1407I BsrGI BssHII BssSI
Bst1107I BstBI BstZ17I CelII CpoI
DraI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI EcoRV FseI HincII
HindII HindIII HpaI KspI MamI
MfeI MluI MunI NheI NotI
NruI NspV PacI PaeR7I PflFI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 1093 GACGTC GACGT^C
Acc65I 2255 GGTACC G^GTACC
AflIII 2819 ACATGT A^C[GA][CT]GT
AgeI 2123 ACCGGT A^CCGGT
ApaI 664 GGGCCC GGGCC^C
Asp718I 2255 GGTACC G^GTACC
AvrII 1959 CCTAGG C^CTAGG
BalI 1311 TGGCCA TGG^CCA
BbvCI 2035 GCTGAGG CCTCAGC(-5/-2)
BcgI 1391 GCAAGAAGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1889 TGATCA T^GATCA
BglII 548 AGATCT A^GATCT
BlnI 1959 CCTAGG C^CTAGG
BsiEI 3363 CGACCG CG[GA][CT]^CG
BspDI 2525 ATCGAT AT^CGAT
BspLU11I 2819 ACATGT A^CATGT
BsrBI 2197 GAGCGG CCGCTC(-3/-3)
BsrDI 2992 CATTGC GCAATG(2/0)
BstAPI 849 GCACTCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 2525 ATCGAT AT^CGAT
DrdI 1141 GACAAGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 2275 ATGCAT ATGCA^T
FbaI 1889 TGATCA T^GATCA
KpnI 2255 GGTACC GGTAC^C
MluNI 1311 TGGCCA TGG^CCA
MscI 1311 TGGCCA TGG^CCA
NaeI 3714 GCCGGC GCC^GGC
NgoMIV 3714 GCCGGC G^CCGGC
NsiI 2275 ATGCAT ATGCA^T
PinAI 2123 ACCGGT A^CCGGT
PshAI 515 GACCACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 1085 AGTACT AGT^ACT
SfaNI 2132 GCATC GCATC(5/9)
SmaI 2294 CCCGGG CCC^GGG
TfiI 2784 GAATC G^A[AT]TC
XmaCI 2294 CCCGGG C^CCGGG
XmaI 2294 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 3628 GTCTAC GT^[AC][GT]AC
3664 GTCTAC
AccIII 312 TCCGGA T^CCGGA
2204 TCCGGA
AvaI 2294 CCCGGG C^[CT]CG[GA]G
2593 CTCGGG
AviII 953 TGCGCA TGC^GCA
1418 TGCGCA
BamHI 872 GGATCC G^GATCC
2201 GGATCC
BbeI 1945 GGCGCC GGCGC^C
3415 GGCGCC
BglI 925 GCCATCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1450 GCCCAGGAGGC
BsaAI 2362 CACGTA [CT]AC^GT[GA]
3667 TACGTG
BseAI 312 TCCGGA T^CCGGA
2204 TCCGGA
BsmI 1584 GAATGC GAATGC(1/-1)
1761 GCATTC
BsoBI 2294 CCCGGG C^[CT]CG[GA]G
2593 CTCGGG
BspEI 312 TCCGGA T^CCGGA
2204 TCCGGA
BsrFI 2123 ACCGGT [GA]^CCGG[CT]
3714 GCCGGC
BstEII 1996 GGTGACC G^GT[ACGT]ACC
3492 GGTCACC
BstPI 1996 GGTGACC G^GT[ACGT]ACC
3492 GGTCACC
BstXI 1914 CCAGAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3108 CCAGCAAGGTGG
Cfr10I 2123 ACCGGT [GA]^CCGG[CT]
3714 GCCGGC
EcoO65I 1996 GGTGACC G^GT[ACGT]ACC
3492 GGTCACC
FspI 953 TGCGCA TGC^GCA
1418 TGCGCA
KasI 1945 GGCGCC G^GCGCC
3415 GGCGCC
MroI 312 TCCGGA T^CCGGA
2204 TCCGGA
NarI 1945 GGCGCC GG^CGCC
3415 GGCGCC
NdeI 1264 CATATG CA^TATG
2779 CATATG
SacI 1168 GAGCTC GAGCT^C
1303 GAGCTC
SfoI 1945 GGCGCC GGC^GCC
3415 GGCGCC
XcmI 593 CCATCATAAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3680 CCATGCAGCCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI Alw44I AlwI AlwNI AocI
ApaLI ApoI Asp700I AspHI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI Bsp1286I BspHI BspMI BsrI
BssKI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SexAI SfcI SmlI StuI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmnI