Restriction map for
pF1KA1405
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# NO CUT ENZYMES ( 89 enzymes) #
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Acc65I AccIII AclI AcsI AflII
AgeI AhdI ApaI ApoI AscI
AseI AsnI Asp718I AspEI AvrII
BciVI BclI BfrI BlnI BsaBI
BsaWI BseAI BsiWI BsmBI BsmI
Bsp1407I BspEI BspHI BspLU11I BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I CpoI DraI DrdI
EagI Eam1105I EclXI Eco52I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HincII HindII HpaI KpnI KspI
MamI MfeI MluI MroI MunI
NcoI NheI NotI NsiI NspI
NspV PacI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI ScaI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI SwaI Tsp509I XbaI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AccI 2398 GTCTAC GT^[AC][GT]AC
AflIII 359 ACGTGT A^C[GA][CT]GT
Alw44I 535 GTGCAC G^TGCAC
ApaLI 535 GTGCAC G^TGCAC
Asp700I 288 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1322 TGCGCA TGC^GCA
BamHI 315 GGATCC G^GATCC
BfaI 1828 CTAG C^TAG
BlpI 2113 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2113 GCTCAGC GC^T[ACGT]AGC
BsaI 1689 GGTCTC GGTCTC(1/5)
BsiEI 590 CGGTCG CG[GA][CT]^CG
BspDI 2521 ATCGAT AT^CGAT
CelII 2113 GCTCAGC GC^T[ACGT]AGC
ClaI 2521 ATCGAT AT^CGAT
FspI 1322 TGCGCA TGC^GCA
HindIII 2461 AAGCTT A^AGCTT
MaeI 1828 CTAG C^TAG
MseI 2393 TTAA T^TAA
NdeI 1331 CATATG CA^TATG
NruI 1050 TCGCGA TCG^CGA
PaeR7I 3013 CTCGAG C^TCGAG
PflMI 72 CCAGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SexAI 710 ACCTGGT A^CC[AT]GGT
SfiI 2094 GGCCCCGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 123 CCCGGG CCC^GGG
Tru9I 2393 TTAA T^TAA
Van91I 72 CCAGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 3013 CTCGAG C^TCGAG
XmaCI 123 CCCGGG C^CCGGG
XmaI 123 CCCGGG C^CCGGG
XmnI 288 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AatII 1336 GACGTC GACGT^C
1915 GACGTC
AocI 1666 CCTGAGG CC^T[ACGT]AGG
2017 CCTGAGG
Aor51HI 1447 AGCGCT AGC^GCT
2312 AGCGCT
AspI 181 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1528 GACGATGTC
BbeI 127 GGCGCC GGCGC^C
166 GGCGCC
BbrPI 175 CACGTG CAC^GTG
358 CACGTG
BbsI 1306 GAAGAC GAAGAC(2/6)
1498 GTCTTC
BpuAI 1306 GAAGAC GAAGAC(2/6)
1498 GTCTTC
BsrDI 2841 CATTGC GCAATG(2/0)
812 GCAATG
BsrFI 2130 GCCGGC [GA]^CCGG[CT]
2543 GCCGGC
Bsu36I 1666 CCTGAGG CC^T[ACGT]AGG
2017 CCTGAGG
Cfr10I 2130 GCCGGC [GA]^CCGG[CT]
2543 GCCGGC
DraIII 529 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
538 CACAGCGTG
Eco47III 1447 AGCGCT AGC^GCT
2312 AGCGCT
Eco81I 1666 CCTGAGG CC^T[ACGT]AGG
2017 CCTGAGG
KasI 127 GGCGCC G^GCGCC
166 GGCGCC
NaeI 2130 GCCGGC GCC^GGC
2543 GCCGGC
NarI 127 GGCGCC GG^CGCC
166 GGCGCC
NgoMIV 2130 GCCGGC G^CCGGC
2543 GCCGGC
PflFI 181 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1528 GACGATGTC
PmaCI 175 CACGTG CAC^GTG
358 CACGTG
PmlI 175 CACGTG CAC^GTG
358 CACGTG
SapI 1240 GAAGAGC GCTCTTC(1/4)
2901 GAAGAGC
SfoI 127 GGCGCC GGC^GCC
166 GGCGCC
Tth111I 181 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1528 GACGATGTC
XcmI 464 CCAAGCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
911 CCAAGACGCTCATGG
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# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
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AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BalI BanI BanII BbvCI BbvI
BcgI BcnI BglI BglII BmyI
BpmI BsaAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrI BssHII BssKI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I SbfI ScrFI SfaNI SfcI
SmlI Sse8387I StuI StyI TaqI
TfiI TseI Tsp45I TspRI TthHB8I
XhoII