Restriction map for
pF1KA1381
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# NO CUT ENZYMES ( 87 enzymes) #
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AatII AccI AclI AflII AgeI
Alw44I AocI Aor51HI ApaLI AscI
AvaI AviII AvrII BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BlnI BlpI Bpu1102I BsaBI BsiWI
BsmI BsoBI BspDI BspHI BsrDI
BssHII BssSI Bst1107I BstBI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DrdI Eco47III Eco81I EcoRI EcoRV
FbaI FseI FspI HindIII HpaI
KasI MamI MfeI MluI MunI
NarI NdeI NheI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI RcaI RsrII SalI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SspI StuI SwaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 164 GGTACC G^GTACC
AccIII 381 TCCGGA T^CCGGA
ApaI 305 GGGCCC GGGCC^C
AseI 2266 ATTAAT AT^TAAT
AsnI 2266 ATTAAT AT^TAAT
Asp700I 1975 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 164 GGTACC G^GTACC
AspI 1713 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 1691 GGACC G^G[AT]CC
BamHI 1061 GGATCC G^GATCC
BanI 164 GGTACC G^G[CT][GA]CC
BglII 389 AGATCT A^GATCT
BsaAI 1114 TACGTG [CT]AC^GT[GA]
BseAI 381 TCCGGA T^CCGGA
BsmBI 67 GAGACG CGTCTC(1/5)
BspEI 381 TCCGGA T^CCGGA
BsrFI 277 GCCGGC [GA]^CCGG[CT]
BstAPI 1698 GCAGGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2833 GGTGACC G^GT[ACGT]ACC
BstPI 2833 GGTGACC G^GT[ACGT]ACC
BstXI 738 CCATGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 277 GCCGGC [GA]^CCGG[CT]
DraII 629 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 1738 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 629 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 2833 GGTGACC G^GT[ACGT]ACC
EcoT22I 1728 ATGCAT ATGCA^T
HincII 561 GTCAAC GT[CT]^[GA]AC
HindII 561 GTCAAC GT[CT]^[GA]AC
KpnI 164 GGTACC GGTAC^C
MroI 381 TCCGGA T^CCGGA
NaeI 277 GCCGGC GCC^GGC
NcoI 738 CCATGG C^CATGG
NgoMIV 277 GCCGGC G^CCGGC
NsiI 1728 ATGCAT ATGCA^T
PflFI 1713 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 2266 ATTAAT AT^TAAT
SbfI 930 CCTGCAGG CCTGCA^GG
SexAI 2045 ACCAGGT A^CC[AT]GGT
SfiI 276 GGCCGGCTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 1726 GCATGC GCATG^C
Sse8387I 930 CCTGCAGG CCTGCA^GG
Tth111I 1713 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2106 TCTAGA T^CTAGA
XmnI 1975 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 377 AAATTC [GA]^AATT[CT]
2159 AAATTC
AcyI 232 GACGCC G[GA]^CG[CT]C
1150 GACGCC
AhdI 2864 GACAGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2884 GACAACACGTC
ApoI 377 AAATTC [GA]^AATT[CT]
2159 AAATTC
AspEI 2864 GACAGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2884 GACAACACGTC
BbsI 1078 GAAGAC GAAGAC(2/6)
2881 GAAGAC
BpuAI 1078 GAAGAC GAAGAC(2/6)
2881 GAAGAC
BsaHI 232 GACGCC G[GA]^CG[CT]C
1150 GACGCC
BsaI 886 GAGACC GGTCTC(1/5)
1132 GGTCTC
BsaWI 381 TCCGGA [AT]^CCGG[AT]
557 TCCGGT
BsiEI 185 CGGCCG CG[GA][CT]^CG
284 CGGCCG
Bsp1407I 1539 TGTACA T^GTACA
2232 TGTACA
BspLU11I 1067 ACATGT A^CATGT
1409 ACATGT
BsrBI 1228 CCGCTC CCGCTC(-3/-3)
77 GAGCGG
BsrGI 1539 TGTACA T^GTACA
2232 TGTACA
EagI 185 CGGCCG C^GGCCG
284 CGGCCG
Eam1105I 2864 GACAGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2884 GACAACACGTC
EclXI 185 CGGCCG C^GGCCG
284 CGGCCG
Eco52I 185 CGGCCG C^GGCCG
284 CGGCCG
EcoNI 174 CCTGATCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
267 CCTCCGCCAGG
HaeII 101 AGCGCC [GA]GCGC^[CT]
2415 GGCGCT
Hin1I 232 GACGCC G[GA]^CG[CT]C
1150 GACGCC
MslI 736 CACCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1110 CATGTACGTG
ScaI 254 AGTACT AGT^ACT
2016 AGTACT
SspBI 1539 TGTACA T^GTACA
2232 TGTACA
XcmI 729 CCAGCCACACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1322 CCAAGGAGCTCTTGG
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# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI AspHI BalI BanII
BbvI BcnI BfaI BglI BmyI
BpmI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EaeI EarI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PstI PvuII
RsaI SacI SacII SapI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII