Restriction map for
pF1KA1364
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# NO CUT ENZYMES ( 92 enzymes) #
##################################
AccII AccIII AclI AflII AflIII
AhdI Aor51HI ApaI AscI AseI
AsnI AspEI AviII BamHI BbrPI
BbsI BfrI BpuAI BsaBI BseAI
BsgI BsiEI BsiWI Bsp1407I BspDI
BspEI BspLU11I BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I ClaI CpoI DraI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRV EcoT22I FseI FspI HgaI
HpaI KspI MamI MfeI MluI
MroI MunI MvnI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SexAI
SfiI SfuI SgrAI SnaBI SphI
SspBI SspI StuI SwaI XbaI
XcmI XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AatII 2788 GACGTC GACGT^C
Acc65I 2724 GGTACC G^GTACC
AccI 2311 GTCTAC GT^[AC][GT]AC
AgeI 50 ACCGGT A^CCGGT
Asp718I 2724 GGTACC G^GTACC
AspI 1396 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 516 CCTAGG C^CTAGG
BbvCI 2707 GCTGAGG CCTCAGC(-5/-2)
BcgI 2177 CGACCCAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 653 TGATCA T^GATCA
BglII 1623 AGATCT A^GATCT
BlnI 516 CCTAGG C^CTAGG
BlpI 1005 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1005 GCTCAGC GC^T[ACGT]AGC
BsaAI 2314 TACGTG [CT]AC^GT[GA]
BsmBI 1055 CGTCTC CGTCTC(1/5)
BsmI 2205 GAATGC GAATGC(1/-1)
BspHI 1784 TCATGA T^CATGA
BstXI 2570 CCAACGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1005 GCTCAGC GC^T[ACGT]AGC
DraII 2526 AGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 258 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 49 GACCGGTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2697 CCTGAGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 2526 AGGACCC [GA]G^G[ACGT]CC[CT]
FbaI 653 TGATCA T^GATCA
HincII 1426 GTCAAC GT[CT]^[GA]AC
HindII 1426 GTCAAC GT[CT]^[GA]AC
HindIII 166 AAGCTT A^AGCTT
KpnI 2724 GGTACC GGTAC^C
NcoI 1832 CCATGG C^CATGG
NspI 2287 ACATGC [GA]CATG^[CT]
PflFI 1396 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 50 ACCGGT A^CCGGT
PpuMI 2526 AGGACCC [GA]G^G[AT]CC[CT]
RcaI 1784 TCATGA T^CATGA
SbfI 2532 CCTGCAGG CCTGCA^GG
ScaI 2375 AGTACT AGT^ACT
SmaI 1235 CCCGGG CCC^GGG
SpeI 1173 ACTAGT A^CTAGT
Sse8387I 2532 CCTGCAGG CCTGCA^GG
TfiI 1702 GATTC G^A[AT]TC
Tth111I 1396 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1235 CCCGGG C^CCGGG
XmaI 1235 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Alw44I 249 GTGCAC G^TGCAC
610 GTGCAC
AocI 568 CCTGAGG CC^T[ACGT]AGG
2697 CCTGAGG
ApaLI 249 GTGCAC G^TGCAC
610 GTGCAC
BciVI 473 GTATCC GTATCC(6/5)
1410 GTATCC
BcnI 1235 CCCGG CC^[GC]GG
1236 CCGGG
BsmFI 1190 GGGAC GGGAC(10/14)
2694 GTCCC
BspMI 70 ACCTGC ACCTGC(4/8)
2798 GCAGGT
BsrBI 138 CCGCTC CCGCTC(-3/-3)
2665 GAGCGG
BsrDI 1752 CATTGC GCAATG(2/0)
1541 GCAATG
BsrFI 50 ACCGGT [GA]^CCGG[CT]
2490 ACCGGC
BstAPI 915 GCAGAGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1044 GCAGCAGCTGC
BstYI 1623 AGATCT [GA]^GATC[CT]
1942 AGATCC
Bsu36I 568 CCTGAGG CC^T[ACGT]AGG
2697 CCTGAGG
Cfr10I 50 ACCGGT [GA]^CCGG[CT]
2490 ACCGGC
Eco81I 568 CCTGAGG CC^T[ACGT]AGG
2697 CCTGAGG
MflI 1623 AGATCT [GA]^GATC[CT]
1942 AGATCC
MseI 170 TTAA T^TAA
1868 TTAA
MspA1I 1048 CAGCTG C[AC]G^C[GT]G
2182 CAGCTG
NciI 1235 CCCGG CC^[GC]GG
1236 CCGGG
PflMI 1631 CCATGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2181 CCAGCTGCTGG
PvuII 1048 CAGCTG CAG^CTG
2182 CAGCTG
SacI 100 GAGCTC GAGCT^C
964 GAGCTC
SmlI 85 CTCAAG C^T[CT][GA]AG
157 CTCAAG
Tru9I 170 TTAA T^TAA
1868 TTAA
Van91I 1631 CCATGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2181 CCAGCTGCTGG
XhoII 1623 AGATCT [GA]^GATC[CT]
1942 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbeI BbvI BfaI BglI BmyI
BpmI BsaHI BsaI BsaJI BsaWI
BseRI BsiHKAI BsiYI BslI BsmAI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DsaI EaeI EarI EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MslI MspI MvaI MwoI NarI
NdeII NlaIII NlaIV PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI StyI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I XmnI