Restriction map for
pF1KA1226
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# NO CUT ENZYMES ( 114 enzymes) #
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Acc65I AccI AccIII AclI AflII
AflIII AgeI AhdI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AvaI AviII AvrII BamHI
BbeI BbvCI BcgI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaBI
BsaWI BseAI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I CelII
Cfr10I ClaI CpoI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO109I EcoO65I EcoRI FauI
FseI FspI HaeII HgaI HindIII
HpaI KasI KpnI KspI MamI
MluI MroI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SwaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AatII 1612 GACGTC GACGT^C
AccII 940 CGCG CG^CG
AcsI 1014 GAATTT [GA]^AATT[CT]
AcyI 1612 GACGTC G[GA]^CG[CT]C
Alw44I 1520 GTGCAC G^TGCAC
AocI 478 CCTGAGG CC^T[ACGT]AGG
ApaLI 1520 GTGCAC G^TGCAC
ApoI 1014 GAATTT [GA]^AATT[CT]
AspI 901 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 1501 CACGTG CAC^GTG
BclI 1752 TGATCA T^GATCA
BcnI 8 CCCGG CC^[GC]GG
BsaAI 1501 CACGTG [CT]AC^GT[GA]
BsaHI 1612 GACGTC G[GA]^CG[CT]C
BsaI 1357 GGTCTC GGTCTC(1/5)
BsgI 196 GTGCAG GTGCAG(16/14)
BspMI 1441 GCAGGT ACCTGC(4/8)
BsrDI 106 GCAATG GCAATG(2/0)
BssSI 1414 CTCGTG CACGAG(-5/-1)
BstAPI 1402 GCAGTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 940 CGCG CG^CG
BstXI 1893 CCAATATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 478 CCTGAGG CC^T[ACGT]AGG
CfoI 1728 GCGC GCG^C
DraI 1951 TTTAAA TTT^AAA
DsaI 1928 CCATGG C^C[GA][CT]GG
Eco81I 478 CCTGAGG CC^T[ACGT]AGG
EcoNI 615 CCTCAATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1904 GATATC GAT^ATC
EcoT22I 1507 ATGCAT ATGCA^T
FbaI 1752 TGATCA T^GATCA
HhaI 1728 GCGC GCG^C
Hin1I 1612 GACGTC G[GA]^CG[CT]C
HinP1I 1728 GCGC G^CGC
HincII 1705 GTCAAC GT[CT]^[GA]AC
HindII 1705 GTCAAC GT[CT]^[GA]AC
MfeI 1154 CAATTG C^AATTG
MspA1I 1944 CAGCTG C[AC]G^C[GT]G
MunI 1154 CAATTG C^AATTG
MvnI 940 CGCG CG^CG
NciI 8 CCCGG CC^[GC]GG
NcoI 1928 CCATGG C^CATGG
NsiI 1507 ATGCAT ATGCA^T
PflFI 901 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1975 CCAGAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1095 GACTC GAGTC(4/5)
PmaCI 1501 CACGTG CAC^GTG
PmlI 1501 CACGTG CAC^GTG
PstI 265 CTGCAG CTGCA^G
PvuII 1944 CAGCTG CAG^CTG
SacI 187 GAGCTC GAGCT^C
SapI 664 GCTCTTC GCTCTTC(1/4)
SphI 2103 GCATGC GCATG^C
StuI 2097 AGGCCT AGG^CCT
TaqI 1616 TCGA T^CGA
Tth111I 901 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1616 TCGA T^CGA
Van91I 1975 CCAGAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 954 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwNI 268 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
1403 CAGTGGCTG
AvaII 308 GGACC G^G[AT]CC
1311 GGACC
BalI 869 TGGCCA TGG^CCA
1890 TGGCCA
BanI 11 GGTGCC G^G[CT][GA]CC
1578 GGTGCC
BanII 187 GAGCTC G[GA]GC[CT]^C
529 GGGCTC
BbsI 111 GTCTTC GAAGAC(2/6)
1715 GTCTTC
BpmI 1360 CTCCAG CTGGAG(16/14)
1838 CTCCAG
BpuAI 111 GTCTTC GAAGAC(2/6)
1715 GTCTTC
BsmI 227 GAATGC GAATGC(1/-1)
1030 GAATGC
BspHI 752 TCATGA T^CATGA
1488 TCATGA
BsrI 273 ACTGG ACTGG(1/-1)
1435 CCAGT
Cfr13I 308 GGACC G^G[ACGT]CC
1311 GGACC
EaeI 869 TGGCCA [CT]^GGCC[GA]
1890 TGGCCA
HapII 9 CCGG C^CGG
1392 CCGG
HpaII 9 CCGG C^CGG
1392 CCGG
MluNI 869 TGGCCA TGG^CCA
1890 TGGCCA
MscI 869 TGGCCA TGG^CCA
1890 TGGCCA
MspI 9 CCGG C^CGG
1392 CCGG
RcaI 752 TCATGA T^CATGA
1488 TCATGA
Sau96I 308 GGACC G^G[ACGT]CC
1311 GGACC
SfcI 265 CTGCAG C^T[GA][CT]AG
1280 CTACAG
SmlI 1111 CTCAAG C^T[CT][GA]AG
1138 CTCAAG
SspI 1818 AATATT AAT^ATT
1895 AATATT
TfiI 1618 GATTC G^A[AT]TC
1971 GAATC
Tsp45I 1165 GTCAC ^GT[GC]AC
1499 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
BbvI BciVI BfaI BmyI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BssKI BstF5I BstNI
BstYI Cac8I DdeI DpnII EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV NspI RsaI
Sau3AI ScrFI SfaNI StyI Tru9I
TseI Tsp509I TspRI XcmI XhoII