Restriction map for
pF1KA1170
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII AccI AccIII AclI AflIII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaLI AscI AseI AsnI AspEI
AspI AviII AvrII BalI BamHI
BbrPI BbvCI BcgI BclI BglII
BlnI BsaAI BsaWI BseAI BsiEI
BsiWI BspEI BspHI BspLU11I BssHII
BssSI Bst1107I BstAPI BstBI BstZ17I
CpoI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoRV
FbaI FseI FspI HincII HindII
HpaI KspI MaeII MfeI MluI
MluNI MroI MscI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SwaI Tth111I Van91I XbaI
XhoI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 1488 GGTACC G^GTACC
AcsI 226 GAATTC [GA]^AATT[CT]
AflII 1561 CTTAAG C^TTAAG
ApoI 226 GAATTC [GA]^AATT[CT]
Asp718I 1488 GGTACC G^GTACC
BciVI 920 GTATCC GTATCC(6/5)
BfrI 1561 CTTAAG C^TTAAG
BlpI 1225 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1225 GCTGAGC GC^T[ACGT]AGC
BsaBI 1644 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 685 GAGACC GGTCTC(1/5)
BsmBI 1495 GAGACG CGTCTC(1/5)
BsmI 1523 GAATGC GAATGC(1/-1)
Bsp1407I 1529 TGTACA T^GTACA
BspDI 1681 ATCGAT AT^CGAT
BspMI 1169 ACCTGC ACCTGC(4/8)
BsrBI 1228 GAGCGG CCGCTC(-3/-3)
BsrDI 47 GCAATG GCAATG(2/0)
BsrFI 341 ACCGGC [GA]^CCGG[CT]
BsrGI 1529 TGTACA T^GTACA
BstEII 756 GGTTACC G^GT[ACGT]ACC
BstPI 756 GGTTACC G^GT[ACGT]ACC
BstYI 1083 GGATCT [GA]^GATC[CT]
CelII 1225 GCTGAGC GC^T[ACGT]AGC
Cfr10I 341 ACCGGC [GA]^CCGG[CT]
ClaI 1681 ATCGAT AT^CGAT
DraI 2062 TTTAAA TTT^AAA
EcoO65I 756 GGTTACC G^GT[ACGT]ACC
EcoRI 226 GAATTC G^AATTC
EcoT22I 715 ATGCAT ATGCA^T
HindIII 705 AAGCTT A^AGCTT
KpnI 1488 GGTACC GGTAC^C
MamI 1644 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1083 GGATCT [GA]^GATC[CT]
NcoI 752 CCATGG C^CATGG
NsiI 715 ATGCAT ATGCA^T
SacI 592 GAGCTC GAGCT^C
SapI 933 GAAGAGC GCTCTTC(1/4)
SspBI 1529 TGTACA T^GTACA
SspI 634 AATATT AAT^ATT
Tsp45I 2219 GTCAC ^GT[GC]AC
XcmI 405 CCAAGGTACTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1083 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 15 CGCG CG^CG
94 CGCG
AlwI 1824 GATCC GGATC(4/5)
1083 GGATC
AocI 1636 CCTGAGG CC^T[ACGT]AGG
2001 CCTGAGG
AspHI 592 GAGCTC G[AT]GC[AT]^C
1288 GAGCAC
AvaI 383 CCCGGG C^[CT]CG[GA]G
680 CCCGGG
BbeI 81 GGCGCC GGCGC^C
2162 GGCGCC
BglI 310 GCCCAAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1019 GCCGCCGTGGC
BsgI 274 GTGCAG GTGCAG(16/14)
1429 GTGCAG
BsiHKAI 592 GAGCTC G[AT]GC[AT]^C
1288 GAGCAC
BsoBI 383 CCCGGG C^[CT]CG[GA]G
680 CCCGGG
BstUI 15 CGCG CG^CG
94 CGCG
Bsu36I 1636 CCTGAGG CC^T[ACGT]AGG
2001 CCTGAGG
DsaI 752 CCATGG C^C[GA][CT]GG
1023 CCGTGG
Eco81I 1636 CCTGAGG CC^T[ACGT]AGG
2001 CCTGAGG
HaeII 81 GGCGCC [GA]GCGC^[CT]
2162 GGCGCC
HgaI 1497 GACGC GACGC(5/10)
2217 GCGTC
KasI 81 GGCGCC G^GCGCC
2162 GGCGCC
MslI 1378 CATGAGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1680 CATCGATGTG
MvnI 15 CGCG CG^CG
94 CGCG
NarI 81 GGCGCC GG^CGCC
2162 GGCGCC
NspI 180 GCATGC [GA]CATG^[CT]
938 GCATGC
PvuII 566 CAGCTG CAG^CTG
988 CAGCTG
SfoI 81 GGCGCC GGC^GCC
2162 GGCGCC
SmaI 383 CCCGGG CCC^GGG
680 CCCGGG
SphI 180 GCATGC GCATG^C
938 GCATGC
StuI 317 AGGCCT AGG^CCT
539 AGGCCT
XmaCI 383 CCCGGG C^CCGGG
680 CCCGGG
XmaI 383 CCCGGG C^CCGGG
680 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcyI AfaI AluI ApaI
Asp700I AvaII BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaHI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstXI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
XmnI