Restriction map for
pF1KA1162
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BfaI BfrI BglI
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstXI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRV EcoT22I
FseI FspI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MaeII MamI MfeI MluI
MluNI MroI MscI MseI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SgrAI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
Tru9I Tth111I Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AlwI 50 GGATC GGATC(4/5)
AocI 940 CCTGAGG CC^T[ACGT]AGG
ApaI 898 GGGCCC GGGCC^C
Asp700I 244 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 114 GGTCC G^G[AT]CC
BanII 898 GGGCCC G[GA]GC[CT]^C
BclI 891 TGATCA T^GATCA
BglII 804 AGATCT A^GATCT
BmyI 898 GGGCCC G[AGT]GC[ACT]^C
BsmFI 1041 GGGAC GGGAC(10/14)
Bsp1286I 898 GGGCCC G[AGT]GC[ACT]^C
BspMI 389 ACCTGC ACCTGC(4/8)
BstBI 382 TTCGAA TT^CGAA
BstYI 804 AGATCT [GA]^GATC[CT]
Bsu36I 940 CCTGAGG CC^T[ACGT]AGG
DsaI 184 CCATGG C^C[GA][CT]GG
Eco81I 940 CCTGAGG CC^T[ACGT]AGG
EcoRI 545 GAATTC G^AATTC
FbaI 891 TGATCA T^GATCA
HgaI 141 GACGC GACGC(5/10)
MflI 804 AGATCT [GA]^GATC[CT]
MslI 987 CACAGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 980 CAGCTG C[AC]G^C[GT]G
NcoI 184 CCATGG C^CATGG
NspI 1029 GCATGC [GA]CATG^[CT]
NspV 382 TTCGAA TT^CGAA
PstI 391 CTGCAG CTGCA^G
PvuII 980 CAGCTG CAG^CTG
SexAI 288 ACCAGGT A^CC[AT]GGT
SfcI 391 CTGCAG C^T[GA][CT]AG
SfuI 382 TTCGAA TT^CGAA
SmaI 923 CCCGGG CCC^GGG
SmlI 1008 CTTGAG C^T[CT][GA]AG
SphI 1029 GCATGC GCATG^C
XcmI 134 CCAACTGGACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 804 AGATCT [GA]^GATC[CT]
XmaCI 923 CCCGGG C^CCGGG
XmaI 923 CCCGGG C^CCGGG
XmnI 244 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccII 54 CGCG CG^CG
90 CGCG
AcsI 172 AAATTT [GA]^AATT[CT]
545 GAATTC
ApoI 172 AAATTT [GA]^AATT[CT]
545 GAATTC
AvaI 79 CCCGAG C^[CT]CG[GA]G
923 CCCGGG
BbsI 385 GAAGAC GAAGAC(2/6)
1000 GAAGAC
BpuAI 385 GAAGAC GAAGAC(2/6)
1000 GAAGAC
BsoBI 79 CCCGAG C^[CT]CG[GA]G
923 CCCGGG
BsrI 137 ACTGG ACTGG(1/-1)
439 ACTGG
BstUI 54 CGCG CG^CG
90 CGCG
EaeI 41 TGGCCG [CT]^GGCC[GA]
300 TGGCCG
EarI 793 GAAGAG CTCTTC(1/4)
835 GAAGAG
FauI 21 CCCGC CCCGC(4/6)
4 GCGGG
HaeII 11 GGCGCT [GA]GCGC^[CT]
33 GGCGCT
Ksp632I 793 GAAGAG CTCTTC(1/4)
835 GAAGAG
MvnI 54 CGCG CG^CG
90 CGCG
PleI 537 GACTC GAGTC(4/5)
1003 GACTC
TaqI 383 TCGA T^CGA
428 TCGA
TspRI 258 CAGTG CA[GC]TG[ACGT][ACGT]^
533 CAGTG
TthHB8I 383 TCGA T^CGA
428 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AluI BbvI BcnI BsaJI
BseRI BsiYI BslI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeIII MboI MboII MnlI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
StyI TfiI TseI Tsp45I Tsp509I