Restriction map for
pF1KA1009
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# NO CUT ENZYMES ( 112 enzymes) #
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AccII AccIII AclI AgeI AhdI
Aor51HI ApaI AscI AspEI AspI
AvaI BalI BamHI BbeI BbrPI
BbvCI BclI BcnI BlpI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspEI BspHI BsrBI BsrGI BssHII
BssSI Bst1107I BstEII BstPI BstUI
BstXI BstZ17I CelII CpoI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO109I EcoO65I EcoT22I
FauI FbaI FseI HaeII HpaI
KasI KspI MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI SexAI SfiI SfoI SgrAI
SmaI SnaBI Sse8387I SspBI StuI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 2950 GACGTC GACGT^C
Acc65I 1227 GGTACC G^GTACC
AciI 3879 CCGC CCGC(-3/-1)
AcyI 2950 GACGTC G[GA]^CG[CT]C
AocI 1574 CCTTAGG CC^T[ACGT]AGG
Asp718I 1227 GGTACC G^GTACC
AvaII 2250 GGACC G^G[AT]CC
AviII 1656 TGCGCA TGC^GCA
AvrII 1423 CCTAGG C^CTAGG
BcgI 2061 GCACAAAAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1579 GGATAC GTATCC(6/5)
BglI 3166 GCCAAAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1423 CCTAGG C^CTAGG
BsaHI 2950 GACGTC G[GA]^CG[CT]C
BsgI 3919 CTGCAC GTGCAG(16/14)
BsmFI 2249 GGGAC GGGAC(10/14)
BspDI 1401 ATCGAT AT^CGAT
BspMI 2601 ACCTGC ACCTGC(4/8)
BsrDI 3426 CATTGC GCAATG(2/0)
BsrFI 3923 ACCGGC [GA]^CCGG[CT]
BstBI 2227 TTCGAA TT^CGAA
Bsu36I 1574 CCTTAGG CC^T[ACGT]AGG
CfoI 1657 GCGC GCG^C
Cfr10I 3923 ACCGGC [GA]^CCGG[CT]
Cfr13I 2250 GGACC G^G[ACGT]CC
ClaI 1401 ATCGAT AT^CGAT
DsaI 2738 CCATGG C^C[GA][CT]GG
EaeI 893 TGGCCG [CT]^GGCC[GA]
Eco81I 1574 CCTTAGG CC^T[ACGT]AGG
EcoNI 2850 CCTAGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 1656 TGCGCA TGC^GCA
HapII 3924 CCGG C^CGG
HgaI 1294 GCGTC GACGC(5/10)
HhaI 1657 GCGC GCG^C
Hin1I 2950 GACGTC G[GA]^CG[CT]C
HinP1I 1657 GCGC G^CGC
HincII 387 GTTGAC GT[CT]^[GA]AC
HindII 387 GTTGAC GT[CT]^[GA]AC
HpaII 3924 CCGG C^CGG
KpnI 1227 GGTACC GGTAC^C
MspI 3924 CCGG C^CGG
NcoI 2738 CCATGG C^CATGG
NspV 2227 TTCGAA TT^CGAA
PflMI 672 CCATTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Sau96I 2250 GGACC G^G[ACGT]CC
ScaI 67 AGTACT AGT^ACT
SfuI 2227 TTCGAA TT^CGAA
SpeI 93 ACTAGT A^CTAGT
SphI 476 GCATGC GCATG^C
Van91I 672 CCATTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AccI 1509 GTCTAC GT^[AC][GT]AC
2221 GTAGAC
AflII 40 CTTAAG C^TTAAG
63 CTTAAG
AflIII 972 ACATGT A^C[GA][CT]GT
1086 ACATGT
Alw44I 380 GTGCAC G^TGCAC
1250 GTGCAC
ApaLI 380 GTGCAC G^TGCAC
1250 GTGCAC
BanI 566 GGTGCC G^G[CT][GA]CC
1227 GGTACC
BbsI 688 GAAGAC GAAGAC(2/6)
2179 GAAGAC
BfrI 40 CTTAAG C^TTAAG
63 CTTAAG
BpuAI 688 GAAGAC GAAGAC(2/6)
2179 GAAGAC
BsmI 2937 GAATGC GAATGC(1/-1)
3966 GAATGC
BspLU11I 972 ACATGT A^CATGT
1086 ACATGT
BstAPI 2515 GCAGCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3421 GCACACATTGC
DraI 259 TTTAAA TTT^AAA
1618 TTTAAA
HaeIII 894 GGCC GG^CC
1242 GGCC
HindIII 263 AAGCTT A^AGCTT
653 AAGCTT
MslI 310 CATGAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2932 CATCAGAATG
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# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
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AcsI AfaI AluI AlwI AlwNI
ApoI AseI AsnI Asp700I AspHI
BanII BbvI BfaI BglII BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I DdeI
DpnII EarI EcoRI EcoRII EcoRV
EcoT14I Fnu4HI FokI HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PshBI PstI
PvuII RsaI SacI Sau3AI ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI