Restriction map for
pF1KA1000
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# NO CUT ENZYMES ( 79 enzymes) #
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AatII AccII AccIII AclI AflII
AgeI AscI AspI AviII AvrII
BbeI BbrPI BcgI BclI BfrI
BlnI BsaAI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspEI BsrGI BssHII
BstBI BstUI CpoI DraIII DrdI
EagI EclXI Eco52I FbaI FseI
FspI HincII HindII HpaI KasI
KspI MluI MroI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspV PacI PflFI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacII SalI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI SwaI
Tth111I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Acc65I 1791 GGTACC G^GTACC
AccI 2081 GTATAC GT^[AC][GT]AC
AcyI 4315 GACGCC G[GA]^CG[CT]C
AflIII 1511 ACATGT A^C[GA][CT]GT
AhdI 4315 GACGCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2603 GTGCAC G^TGCAC
AocI 1657 CCTAAGG CC^T[ACGT]AGG
Aor51HI 3797 AGCGCT AGC^GCT
ApaI 1370 GGGCCC GGGCC^C
ApaLI 2603 GTGCAC G^TGCAC
AseI 1468 ATTAAT AT^TAAT
AsnI 1468 ATTAAT AT^TAAT
Asp718I 1791 GGTACC G^GTACC
AspEI 4315 GACGCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 3499 GGATCC G^GATCC
BciVI 2668 GTATCC GTATCC(6/5)
BglII 1599 AGATCT A^GATCT
BsaHI 4315 GACGCC G[GA]^CG[CT]C
BsaWI 4109 ACCGGA [AT]^CCGG[AT]
BspDI 2896 ATCGAT AT^CGAT
BspHI 927 TCATGA T^CATGA
BspLU11I 1511 ACATGT A^CATGT
BsrFI 4934 ACCGGC [GA]^CCGG[CT]
BssSI 4414 CACGAG CACGAG(-5/-1)
Bst1107I 2081 GTATAC GTA^TAC
BstAPI 4290 GCACCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 2081 GTATAC GTA^TAC
Bsu36I 1657 CCTAAGG CC^T[ACGT]AGG
Cfr10I 4934 ACCGGC [GA]^CCGG[CT]
ClaI 2896 ATCGAT AT^CGAT
Eam1105I 4315 GACGCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3797 AGCGCT AGC^GCT
Eco81I 1657 CCTAAGG CC^T[ACGT]AGG
EcoRI 3306 GAATTC G^AATTC
FauI 5555 CCCGC CCCGC(4/6)
HgaI 4315 GACGC GACGC(5/10)
Hin1I 4315 GACGCC G[GA]^CG[CT]C
KpnI 1791 GGTACC GGTAC^C
MfeI 1503 CAATTG C^AATTG
MunI 1503 CAATTG C^AATTG
PflMI 3456 CCAAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 4647 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1468 ATTAAT AT^TAAT
RcaI 927 TCATGA T^CATGA
ScaI 1912 AGTACT AGT^ACT
SphI 806 GCATGC GCATG^C
StuI 3330 AGGCCT AGG^CCT
Van91I 3456 CCAAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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Asp700I 2155 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4757 GAAAGCTTTC
BcnI 4871 CCCGG CC^[GC]GG
5240 CCCGG
BglI 5299 GCCAAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5311 GCCATTGAGGC
BsaBI 2424 GATGCGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4977 GATTCAAATC
BsaI 4000 GAGACC GGTCTC(1/5)
409 GGTCTC
BsrBI 4341 CCGCTC CCGCTC(-3/-3)
5059 GAGCGG
BstEII 1293 GGTAACC G^GT[ACGT]ACC
4866 GGTTACC
BstPI 1293 GGTAACC G^GT[ACGT]ACC
4866 GGTTACC
DsaI 1040 CCATGG C^C[GA][CT]GG
4244 CCATGG
EcoO65I 1293 GGTAACC G^GT[ACGT]ACC
4866 GGTTACC
EcoRV 829 GATATC GAT^ATC
2998 GATATC
EcoT22I 2049 ATGCAT ATGCA^T
5571 ATGCAT
HaeII 374 GGCGCT [GA]GCGC^[CT]
3797 AGCGCT
HindIII 1589 AAGCTT A^AGCTT
4759 AAGCTT
MaeII 22 ACGT A^CGT
4692 ACGT
MamI 2424 GATGCGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4977 GATTCAAATC
NciI 4871 CCCGG CC^[GC]GG
5240 CCCGG
NcoI 1040 CCATGG C^CATGG
4244 CCATGG
NsiI 2049 ATGCAT ATGCA^T
5571 ATGCAT
PaeR7I 3419 CTCGAG C^TCGAG
3643 CTCGAG
SexAI 2076 ACCAGGT A^CC[AT]GGT
4577 ACCAGGT
XhoI 3419 CTCGAG C^TCGAG
3643 CTCGAG
XmnI 2155 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4757 GAAAGCTTTC
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# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DraII EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII